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Trichoderma longibrachiatum
About: Trichoderma longibrachiatum is a research topic. Over the lifetime, 452 publications have been published within this topic receiving 10591 citations.
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TL;DR: It is suggested that AGH is a novel enzyme and is useful for specifically diagnosing diabetes mellitus.
Abstract: A novel NAD-dependent dehydrogenase highly specific for 1,5-anhydro-d-glucitol (1,5-AG) was found in the cell extract of an imperfect fungus, Trichoderma longibrachiatum strain 11-3. This fungus used 1,5-AG as a sole carbon source for growth and transformed 1,5-AG into glucose. 1,5-AG dehydrogenase (AGH) was purified to homogeneity, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The molecular mass of the purified enzyme was estimated to be 36 and 141 kDa by SDS-PAGE and by gel filtration, respectively, suggesting that the enzyme was homotetrameric. The enzyme was highly specific for 1,5-AG and did not exhibit activity with any sugar or sugar alcohol tested in this study other than 1,5-AG. A linear relationship between the initial rate of the enzyme reaction and the concentration of 1,5-AG at the physiological level was observed. The presence of glucose in abundance did not interfere with the relationship. The optimum temperature for the enzyme reaction was 50°C, and the enzyme was stable at temperatures up to 70°C. These results suggested that AGH is a novel enzyme and is useful for specifically diagnosing diabetes mellitus.
6 citations
6 citations
6 citations
TL;DR: It is demonstrated that there is considerable opportunity for using native BCAs against G. lucidum in managing root rot disease and studies on compatibility between insecticides and BCAs suggests that T. longibrachiatum was more effective in inhibiting mycelium growth compared to other BCAs.
Abstract: This study investigates the potential of native biocontrol agents (BCAs) as controls against Ganoderma lucidum causing root rot mortality in Indian mesquite. The disease is prevalent in sandy soils where trees grow under rainfed conditions. In addition, a beetle namely Acanthophorus serraticornis damages the roots, resulting in increasing vulnerability of the host thereby allowing easy of the pathogen. In dual culture tests, Ganoderma infected cowpea root bit experiment and compatibility with insecticides revealed that the three BCAs (Trichoderma longibrachiatum, T. harzianum, and Aspergillus nidulans) significantly inhibited G. lucidum mycelial growth. The highest mycelial growth inhibition (47.6%) was recorded after 96 h followed by 39.8% and 29.3% at 72 and 48 h, respectively, by T. longibrachium. Cell free filtrates of T. longibrachiatum, T. harzianum, and A. nidulans were superior in inhibiting mycelium growth. A low concentration (3 ml) of T. longibrachiatum was more effective in inhibiting mycelium growth compared to other BCAs. Both Prosopis juliflora compost and onion residue compost amendments as food substrates favored the growth of these BCAs, which ultimately reduced the viability of Ganoderma-colonized root bits of cowpea. Studies on compatibility between insecticides and BCAs suggests that T. longibrachiatum, harzianum and A. nidulans can be combined with phorate or chloropyriphos (both organophosphates) at variable concentrations if amended together for partially infected trees, or as a prophylactic measure in healthy trees. These studies demonstrate that there is considerable opportunity for using native BCAs against G. lucidum in managing root rot disease.
6 citations
TL;DR: In this article, a short peptaibol produced by Trichoderma longibrachiatum was tested against Pyricularia oryzae, the causal agent of rice blast disease.
Abstract: Eco-friendly analogs of Trichogin GA IV, a short peptaibol produced by Trichoderma longibrachiatum, were assayed against Pyricularia oryzae, the causal agent of rice blast disease. In vitro and in vivo screenings allowed us to identify six peptides able to reduce by about 70% rice blast symptoms. One of the most active peptides was selected for further studies. Microscopy analyses highlighted that the treated fungal spores could not germinate and the fluorescein-labeled peptide localized on the spore cell wall and in the agglutinated cytoplasm. Transcriptomic analysis was carried out on P. oryzae mycelium 3 h after the peptide treatment. We identified 1,410 differentially expressed genes, two-thirds of which upregulated. Among these, we found genes involved in oxidative stress response, detoxification, autophagic cell death, cell wall biogenesis, degradation and remodeling, melanin and fatty acid biosynthesis, and ion efflux transporters. Molecular data suggest that the trichogin analogs cause cell wall and membrane damages and induce autophagic cell death. Ultrastructure observations on treated conidia and hyphae confirmed the molecular data. In conclusion, these selected peptides seem to be promising alternative molecules for developing effective bio-pesticides able to control rice blast disease.
6 citations