Trichoderma longibrachiatum

About: Trichoderma longibrachiatum is a research topic. Over the lifetime, 452 publications have been published within this topic receiving 10591 citations.

Case Report: Trichoderma longibrachiatum infections in a pediatric patient with peritoneal dialysis

Abstract: A case report of an intrahospitalary Trichoderma longibrachiatum peritonitis in a female 13-year-old pediatric patient who had been previously diagnosed a terminal chronic renal insufficiency while being under peritoneal dialysis is presented. Nowasays literature recognizes certain members of the genus Trichoderma to be oportunistic pathogens of increasing occurrence in immunocompromised patients. The above girl recived a satisfactory Amphotericin B treatment yet she died due to an hypertensive emergency appearing secondarily to her basic pathology.

Using barium ions for heavy-atom derivatization and phasing of xylanase II from Trichoderma longibrachiatum.

Abstract: This paper describes the use of barium chloride to produce a heavy-atom derivative of xylanase II crystals from Trichoderma longibrachiatum, which was obtained either by cocrystallization or soaking. SAD phasing led to interpretable electron-density maps that allowed unambiguous chain tracing. In the best case, with a data set collected at 9.5 keV, 88% of the residues were built, with 83% of the side chains assigned. The barium ions are found to mainly interact with main-chain carbonyl groups and water molecules. It is suggested that barium ions could also be used as a potential anomalous scatterer in the quick cryosoaking procedure for phasing.

Trichoderma Transformation Methods

Abstract: Trichoderma includes a huge variety of fungal species with an increasing interest from several points of view. Many of their species produce primary or secondary metabolites with importance in pharmaceutical industry or in the biocontrol of significant phytopathogenic fungi (Cardoza et al., Curr Genet 34:50–59, 2005; Sivasithamparam and Ghisalberti, Trichoderma and Gliocladium, London, pp 139–191). Other Trichoderma species produce enzymes with a remarkable industrial importance and even other strains from Trichoderma brevicompactum (Tijerino et al., Fungal Genet Biol 48:285–296, 2011a, Toxins (Basel) 3:1220–1232, 2011b) or Trichoderma longibrachiatum (Alanio et al., Clin Infect Dis 46:e116–118, 2008) have potential pathogenic activity against plants and animals, including human beings, respectively. The practical applications of this genus have resulted in an increasing interest in the development of efficient transformation procedures, in order to select transgenic strains with valuable phenotypes. These procedures should be essential tools to characterize the physiological roles of the increasing number of genes available as a result of the fungal genomic projects (http://genome.jgi-psf.org/programs/fungi) (Grigoriev et al., Nucleic Acids Res 40:D26–32, 2012).