Trichoderma longibrachiatum

About: Trichoderma longibrachiatum is a research topic. Over the lifetime, 452 publications have been published within this topic receiving 10591 citations.

Isolation and identification of South African indigenous fungal strains and assessment of their ability to degrade diclofenac in synthetic wastewater

Abstract: Pharmaceutical compounds barely removed from wastewater have become a huge concern due to their adverse effects on all environments, human health and wildlife. Among the treatment techniques, white-rot fungi (WRF) has shown a high diclofenac sodium (DCF) removal efficiency. The aims of this study were to isolate and identify South African fungi, to culture and optimize their growth, and to assess their ability to remove DCF from synthetic wastewater. Simple microscopic and FEG-SEM analysis were used followed by PCR analysis performed using ITS1/ITS4, EF1/EF2 and s-tubulin primers. Five fungal species closely related (approximately 98 to 100% similarity) to Trichoderma longibrachiatum, Trametes polyzona, Aspergillus niger, Mucor circinelloides and Rhizopus microsporus, were successfully isolated and identified. The highest growth (8.7±0.07 cm) was observed with T. longibrachiatum in culture medium composed of glucose-anhydrous-agar-peptone at 30±1.5 °C. The optimum C/N ratio of growth was found at 5.5:1. After 5 days, the highest DCF removal efficiency from a concentration of 10 mg/L was recorded for R. microsporus (72.29±3.5%), followed by A. niger (61.2±4.5%), M. circinelloides (61.2±3.5%), T. polyzona (22.42±4.5%), and by T. longibrachiatum (0%). Apart from the WRF, this study showed that other fungal strains can also be used for the degradation of recalcitrant pharmaceuticals.

Statistical optimization of Lglutaminase production by Trichoderma species under solid state fermentation using African locust beans as substrate

Abstract: Six strains of Trichoderma species isolated from soil and air samples were screened for extracellular L-glutaminase production. The highest enzyme producing isolate, Trichoderma longibrachiatum was subjected to screening and nutrients optimization experiments under solid state fermentation using a constant weight of African locust beans as substrate. A specific activity of 54.96 U/mg was achieved at 1% (w/v) glutamine, 0.5% (w/v) glucose, 1% (w/v) NaCl, pH 3.0 and 1.4 ml inoculums size respectively after 3 days of incubation at 27°C as the optimum condition for L–glutaminase production. Asides glutamine, other nitrogen sources; malt extract and ammonium sulphate had a negative influence on enzyme production likewise the supplemented sources of metal ions; zinc and iron (II) ions. Key words: Trichoderma, L-glutaminase, fermentation, African locust beans.

Preparation method and application of water dispersible granules containing Trichoderma longibrachiatum T6 as biocontrol bacterium

Abstract: The invention discloses a preparation method for successfully developing water dispersible granules containing Trichoderma longibrachiatum T6 by utilizing an antagonistic bacterium, namely, Trichoderma longibrachiatum T6 separated from soil as a research object, optimizing a culture medium formula and screening auxiliary types, and storage stability of the water dispersible granules is measured. The water dispersible granules in the formula comprise components as follows: a master batch prepared from grass peat, vermiculite and cow dung as well as an auxiliary prepared from carboxymethyl cellulose, calcium carbonate, polyvinyl pyrrolidone, soluble starch, attapulgite and lauryl sodium sulfate. Tests prove that the water dispersible granules containing Trichoderma longibrachiatum T6 as thebiocontrol bacterium have remarkable growth-promoting effect on cucumbers, tomatoes, hot peppers, oilseed rapes and Chinese cabbages and have obvious biological control effect on occurrence and harm of cucumber rhizoctonia rot, rhizoctonia rot of peppers and phoma root rot of colza.