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Trichoderma longibrachiatum

About: Trichoderma longibrachiatum is a research topic. Over the lifetime, 452 publications have been published within this topic receiving 10591 citations.


Papers
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Journal ArticleDOI
TL;DR: Dynamic light scattering and kinetic studies indicate that likely factors in the loss of enzyme activity for this xylanase are the dampening of dynamic motion and kinetic trapping of cations in the binding pocket as opposed to the denaturing of the protein.
Abstract: We have discovered that a family 11 xylanase from Trichoderma longibrachiatum maintains significant activity in low concentrations of the ionic liquids (IL) 1-ethyl-3-methyl-imidazolium acetate ([E...

86 citations

01 Jan 2008
TL;DR: The results obtained from the fermentations showed that Trichoderma longibrachiatum produced the highest amount of glucose among the cultures tested, and this was produced from pineapple pulp at pH 4.5 and temperature of 45oC on Day7 of fermentation.
Abstract: Cellulase production from cellulosic pineapple waste using Trichoderma longibrachiatum, Aspergillus niger and Saccharomyces cerevisiae was assessed. The wastes were dried, pre-treated with alkali and steam, re-dried and then blended. The powdered wastes were then used as substrates in separate shake-flasks which contained mineral salts medium (MSM) and inoculi of Trichoderma longibrachiatum, Aspergillus niger and Saccharomyces cerevisiae. Fermentations were carried out in flasks containing the MSM, the waste substrate and the inoculum at pH 5.0, 1% substrate concentration, 10% inoculum size and cultured on a rotary shaker at 29±1oC initially for 5 days to verify cellulase production by the organisms from the waste substrates, then for 7 days or 9 days while varying different fermentation parameters. Cellulase activity and amount of glucose produced by the three test organisms from the waste substrates were determined and compared. The amount of glucose produced was optimized by varying the fermentation parameters: Time, pH, Substrate concentration, Inoculum size and Temperature. The results obtained from the fermentations showed that Trichoderma longibrachiatum produced the highest amount of glucose among the cultures tested (0.92mg/0.5ml). This was produced from pineapple pulp at pH 4.5 and temperature of 45oC on Day7 of fermentation. The highest amount of glucose produced by Aspergillus niger was also from pineapple pulp (0.63mg/0.5ml) at pH 3.5 and temperature of 40oC on Day5 of fermentation. The highest amount of glucose produced by Saccharomyces cerevisiae was from pineapple pulp (0.54mg/0.5ml) at pH 4.5 and temperature of 45oC on Day5 of fermentation. (Nature and Science. 2008;6(2):64-79). ISSN: 1545-0740.

83 citations

Journal ArticleDOI
TL;DR: While Trichoderma species have been recognized to be pathogenic in profoundly immunosuppressed hosts with increasing frequency, this is the first report of probable acquisition through the gastrointestinal tract.
Abstract: Trichoderma longibrachiatum was recovered from stool surveillance cultures and a perirectal ulcer biopsy specimen from a 29-year-old male who had received an allogeneic bone marrow transplant for acute lymphoblastic leukemia The amphotericin B (20 μg/ml) and itraconazole (10 μg/ml) MICs for the organism were elevated Therapy with these agents was unsuccessful, and the patient died on day 58 posttransplantation At autopsy, histologic sections from the lungs, liver, brain, and intestinal wall showed infiltration by branching septate hyphae Cultures were positive for Trichoderma longibrachiatum While Trichoderma species have been recognized to be pathogenic in profoundly immunosuppressed hosts with increasing frequency, this is the first report of probable acquisition through the gastrointestinal tract Salient features regarding the identification of molds in the Trichoderma longibrachiatum species aggregate are presented

82 citations

Patent
16 Aug 1995
TL;DR: In this article, an enzyme feed additive is provided comprising a xylanase, a protease, and optionally a β-glucanase, where the ratio of the units of x-layer activity per unit amount of the feed additive to β-layer activation is 1:0-0.25.
Abstract: An enzyme feed additive is provided comprising a xylanase, a protease, and optionally a β-glucanase. The ratio of the units of xylanase activity per unit amount of the feed additive to the units of β-glucanase activity per same unit amount of the feed additive is 1:0-0.25. Preferably, the xylanase is the low pI xylanase and/or the high pI xylanase obtained from Trichoderma longibrachiatum. Preferably, the protease is a mutant subtilisin comprising a substitution at the amino acid residue position equivalent to tyr+217 of Bacillus amyloliquefaciens subtilisin with leucine.

80 citations

Journal ArticleDOI
TL;DR: Findings support the development of novel strategies of integrated control of aphid pests by investigating the influence of root colonization by a nonmycorrhizal plant-growth-promoting fungus on direct and indirect defenses of tomato plant against aphids.
Abstract: Below ground and above ground plant-insect-microorganism interactions are complex and regulate most of the developmental responses of important crop plants such as tomato. We investigated the influence of root colonization by a nonmycorrhizal plant-growth-promoting fungus on direct and indirect defenses of tomato plant against aphids. The multitrophic system included the plant Solanum lycopersicum ('San Marzano nano'), the root-associated biocontrol fungus Trichoderma longibrachiatum strain MK1, the aphid Macrosiphum euphorbiae (a tomato pest), the aphid parasitoid Aphidius ervi, and the aphid predator Macrolophus pygmaeus. Laboratory bioassays were performed to assess the effect of T. longibrachiatum MK1, interacting with the tomato plant, on quantity and quality of volatile organic compounds (VOC) released by tomato plant, aphid development and reproduction, parasitoid behavior, and predator behavior and development. When compared with the uncolonized controls, plants whose roots were colonized by T. longibrachiatum MK1 showed quantitative differences in the release of specific VOC, better aphid population growth indices, a higher attractiveness toward the aphid parasitoid and the aphid predator, and a quicker development of aphid predator. These findings support the development of novel strategies of integrated control of aphid pests. The species-specific or strain-specific characteristics of these below ground-above ground interactions remain to be assessed.

78 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20221
202121
202026
201926
201819
201723