Trichoderma longibrachiatum

About: Trichoderma longibrachiatum is a research topic. Over the lifetime, 452 publications have been published within this topic receiving 10591 citations.

Microbial inoculant and an extract of Trichoderma longibrachiatum with xylanase activity effect on chemical composition,fermentative profile and aerobic stabilityof guinea grass ( Pancium maximum Jacq.) silage

Abstract: Tropical grasses, such as guinea grass (Panicum maximum Jacq), are relatively high yielding, exhibit perennial growth, and may be conserved for periods of forage shortage. However, tropical grasses have low contents of DM and soluble carbohydrates, high buffering capacity, and can contain large air volumes trapped within the silo. These features result in prolonged plant respiration and aerobic microbial ABSTRACT. The objective of this study was to determine the effects of increasing dose of a microbial inoculant alone or in combination with a Trichoderma longibrachiatum extract with xylanase activity on total losses, chemical composition, fermentative profile, microbiological quality and aerobic stability of guinea grass (Panicum maximum Jacq. cv. Mombasa) silage. Sixty minisilos (0.022 m3) were used in a 3 × 2 factorial experiment, composed by three levels (0, 4 or 8 g · t−1 of fresh forage) of microbial inoculant (INO) and two levels (0 or 1 IU · g−1 of fresh forage) of enzyme product (ENZ). INO consisted of Lactobacillus plantarum at 4 × 1010 cfu · g−1 and Pediococcus acidilactici at 4 × 1010 cfu · g−1. Silos were opened after 60 days. The combination of INO8 with ENZ caused the lowest gas losses. ENZ increased silage crude protein content, as well as the dry matter and neutral detergent fibre (NDF) in vitro digestibility. INO doses exerted a positive quadratic effect on NDF in vitro digestibility. ENZ addition increased acetic acid concentration, while INO treatments linearly decreased acetic and butyric acid concentrations and linearly increased lactic and propionic acid concentrations in silage. INO exhibited a negative quadratic effect on pH and NH3-N concentration of guinea grass silage and positive linear increase in the counts of anaerobic bacteria. Combinations of ENZ and INO8 decreased silage aerobic stability. Although there was observed no combined effect of ENZ and INO on silage chemical composition and fermentative profile, they exerted positive influence on NDF in vitro digestibility of the guinea grass silage when added alone (ENZ and INO at a dose of 4 g · t−1). Received: 22 April 2017 Revised: 1 August 2017 Accepted: 4 December 2017

Biocontrol of Mushroom Spoilage Fungi andAflatoxin Evaluation During Storage

Abstract: The use of chemical substances in the control of pathogens is relatively expensive, it constitutes environmental hazards coupled with high level of toxicity to humans and it is therefore considered unsafe. Studies were carried out to investigate the biocontrol of fungi causing spoilage in mushroom and aflatoxin production during storage. The results obtained revealed that four species of fungi namely Aspergillus fumigatus, Aspergillus niger, Botryodiplodia theobromae and Rhizopus stolonifer were isolated from the spoilt mushroom samples. The antagonistic activities of four biocontrol agents; Trichoderma asperellum CMI T158, Trichoderma longibrachiatum CMI T167, Pseudomonas fluorescence CMI F113 and Bacillus subtilis CMI 22BN against the isolated spoilage fungi were tested using the agar well diffusion method. The optimization of the antagonistic activities of the biocontrol agent metabolites was carried out based on pH and temperature variations. The heat stability of the metabolites produced by the biocontrol agents and the aflatoxin production during storage of the mushroom samples were evaluated. The results obtained revealed that Bacillus subtilis did not inhibit Aspergillus fumigatus, but inhibited Aspergillus niger, Botryodiplodia theobromae and Rhizopus stononifer with inhibition zones of 25mm 0.18, 21mm0.11 and 20mm0.13 respectively. Pseudomonas fluorescence and Trichoderma longibrachiatum inhibited all the tested pathogens, while Trichoderma asperellum did not inhibit A. fumigatus and A. niger but inhibited B. theobromae and R. stolonifer showing inhibition zones of 22mm 0.15 and 20mm 014 respectively. The effect of varying pH on the inhibitory activity of the metabolites produced by the biocontrol agents showed that the optimum inhibitory ability of the bacterial biocontrol agents were enhanced at pH 6.0, while the fungal biocontrol agents showed maximum inhibitory activity at pH 5.0. However variations in temperatures revealed that both the bacterial and fungal biocontrol agents were more effective at 30 0 C. The monitoring of the thermal stability of the metabolites produced by the biocontrol agents indicated that they were inhibitory at 20

Effects of Trichoderma longibrachiatum (NGJ167) and compost on early growth of Bougainvillea spectabilis

Abstract: Bougainvillea spectabilis is an ornamental plant grown in both temperate and tropical zones. They are primarily propagated by stem cuttings; however, difficulty in growth at early stages occurs routinely, causing barrier for its mass propagation. This study was conducted to study the effects of integrating compost with Trichoderma longibrachiatum (NGJ167), in improving the early growth of Bougainvillea spectabilis . Trichoderma longibrachiatum (NGJ167) used for this purpose was obtained from the Bioscience Center, International Institute of Tropical Agriculture (IITA), Ibadan. One hundred and twenty (120) pots were filled with 5 kg top soil each from an alfisol. Treatments included: Top soil as Control (C), Top soil inoculated with T. longibrachiatum (T), Top soil + compost at 5, 10 and 15 t ha -1 as C1, C2, C3 respectively, Top soil inoculated with T. longibrachiatum + compost at 5, 10 and 15 t ha -1 as C1T, C2T, C3T respectively. The eight treatments were arranged in a completely randomized design (CRD) and replicated three times. Results indicated that integrated use of compost with Trichoderma significantly (P < 0.05) had higher growth values than the control. Higher plant height, stem diameter, number of branches and number of leaves, were recorded with the use of compost at 10 t ha -1 compared to control. Compost with Trichoderma gave higher shoot dry weight compared to treatments of compost alone. In conclusion, use of compost alone or inoculated with Trichoderma had significant effect on early growth and development of Bougainvillea spectabilis.