scispace - formally typeset
Search or ask a question
Topic

Trichoderma longibrachiatum

About: Trichoderma longibrachiatum is a research topic. Over the lifetime, 452 publications have been published within this topic receiving 10591 citations.


Papers
More filters
Journal ArticleDOI
TL;DR: The potential use of xylanases to synthesize AuNPs is demonstrated, which to the best knowledge, is the first report of such, and the biosynthesized nanoparticles displayed activities making it predisposed for potential use as excellent biomedical agents.
Abstract: The involvement of enzymes in green nanotechnology is rapidly developing and has continued to blossom in recent times. The present study examines the use of fungal xylanases from Aspergillus niger L3 (NEA) and Trichoderma longibrachiatum L2 (TEA) to biosynthesize gold nanoparticles (AuNPs) for biomedical applications. AuNPs were synthesized with indication of colour change from light yellow to purple, and surface plasmon resonance at 545 and 560 nm were obtained for NEA-AuNPs and TEA-AuNPs respectively. The Fourier-transform infrared spectroscopy indicated that protein molecules were responsible for the capping and stabilization of the nanoparticles, which were mainly spherical (NEA-AuNPs) and flower-shaped (TEA-AuNPs). The sizes of the nanoparticles ranged from 4.88 to 123.99 nm, and displayed maximum antibacterial activity of 44.3% at 100 µg/ml, and antifungal activity of 87% at 150 µg/ml. While the AuNPs scavenged DPPH by 53.79%, hydrogen peroxide was scavenged by 96%. The biosynthesized AuNPs showed excellent anticoagulant and thrombolytic activities on human blood. This study demonstrated the potential use of xylanases to synthesize AuNPs, which to the best our knowledge, is the first report of such. The biosynthesized nanoparticles displayed activities making it predisposed for potential use as excellent biomedical agents.

76 citations

Journal ArticleDOI
TL;DR: The nematotoxic activity, efficient against plant-parasitic species, found in the culture filtrates of two Hyphomycetes: Paecilomyces lilacinus (nematophagous fungus) and Trichoderma longibrachiatum was characterized and selective permeability of different cuticle zones of nematodes for non-charged substances (non-polar) was confirmed.
Abstract: The nematotoxic activity, efficient against plant-parasitic species, found in the culture filtrates of two Hyphomycetes: Paecilomyces lilacinus (nematophagous fungus) and Trichoderma longibrachiatum was characterized. Purification was performed by distillation and liquid chromatography and identification by gas-chromatography coupled with a DCI system (dynamic headspace). The active substance has been identified as acetic acid which, produced abundantly during fungal growth in liquid medium (up to 0.044 mols of CH 3 COOH per culture filtrate litre), inhibited movement of infesting juvenile nematodes. These results were confirmed by comparative studies using the commercial molecule as for the 1 H NMR as the biological tests. The toxic activity appeared only when the carboxylic function was in its undissociated form, hence the real interest of pH when studying this nematicidal activity. A study of the literature confirmed the selective permeability of different cuticle zones of nematodes for non-charged substances (non-polar).

76 citations

Journal ArticleDOI
TL;DR: It is demonstrated that besides mycoparasitism, VOCs play a major role in antagonism of pathogenic fungi by four endophytic fungi belonging to the genus Trichoderma.

75 citations

Journal ArticleDOI
TL;DR: An electrophoretic karyotype of Trichoderma longibrachiatum (reesei) was obtained using contourclamped homogeneous electric field (CHEF) gel electrophoresis and three hyper-cellulolytic mutant strains, which showed striking differences in their karyotypes compared to the initial parent.
Abstract: An electrophoretic karyotype of Trichoderma longibrachiatum (reesei) was obtained using contourclamped homogeneous electric field (CHEF) gel electrophoresis. Seven chromosomal DNA bands were separated in the wild-type T. longibrachiatum strain QM6a. The sizes of the chromosomal DNA bands ranged from 2.8 to 6.9 Mb, giving an estimated total genome size of about 33 Mb. The electrophoretic karyotype of the strain QM6a was compared to three hyper-cellulolytic mutant strains, QM9414, RutC30 and VTT-D-79125. The chromosome pattern of the mutant QM9414 was quite similar to that of the wild-type QM6a except that the smallest chromosome differed somewhat in size. The VTT-D-79125 and RutC30 strains, which have undergone several mutagenesis steps, showed striking differences in their karyotype compared to the initial parent. The chromosomal DNA bands were identified using the previously characterized T. longibrachiatum genes (egl1, egl2, cbh1, cbh2, pgk1, rDNA) and random clones isolated from a genomic library. In all strains the cellulase genes cbh1, cbh2 and egl2 were located in the same linkage group (chromosome II in the wild-type), while the main endoglucanase, egl1, hybridized to another chromosomal DNA band (chromosome VI in the wild-type).

73 citations


Network Information
Related Topics (5)
Bacillus subtilis
19.6K papers, 539.4K citations
76% related
Chitin
6.5K papers, 253.9K citations
76% related
Bacteria
23.6K papers, 715.9K citations
74% related
Cellulase
16.1K papers, 479.5K citations
74% related
Yeast
31.7K papers, 868.9K citations
74% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20221
202121
202026
201926
201819
201723