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Trichoderma reesei

About: Trichoderma reesei is a research topic. Over the lifetime, 3832 publications have been published within this topic receiving 152877 citations. The topic is also known as: Trichoderma reesi.


Papers
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Journal ArticleDOI
TL;DR: Low-cost mixture was produced from glucose through the transglycosylation reaction catalyzed by β-glucosidase for cellulase overproduction by Trichodema reesei RUT C30, and cellulase titer of 90.3FPU/mL, which was more than 10 folds of that achieved with lactose as inducer, was achieved.

81 citations

Journal ArticleDOI
TL;DR: The feasibility of improving cellulase production by modifying regulator expression is demonstrated, an attractive new single-step approach for increasing total cellulase productivity in T. reesei is suggested, and very high levels of CMCase activity during growth on glucose are found.
Abstract: To investigate whether enzyme production can be enhanced in the Trichoderma reesei industrial hyperproducer strain RUT C30 by manipulation of cellulase regulation, the positive regulator Xyr1 was constitutively expressed under the control of the strong T. reesei pdc promoter, resulting in significantly enhanced cellulase activity in the transformant during growth on cellulose. In addition, constitutive expression of xyr1 combined with downregulation of the negative regulator encoding gene ace1 further increased cellulase and xylanase activities. Compared with RUT C30, the resulting transformant exhibited 103, 114, and 134 % greater total secreted protein levels, filter paper activity, and CMCase activity, respectively. Surprisingly, strong increases in xyr1 basal expression levels resulted in very high levels of CMCase activity during growth on glucose. These findings demonstrate the feasibility of improving cellulase production by modifying regulator expression, and suggest an attractive new single-step approach for increasing total cellulase productivity in T. reesei.

81 citations

Journal ArticleDOI
TL;DR: Perfect mitotic stability was found in half of the non-abortive transformants, correlating with vector integration at homologous and ectopic loci, and in the unstable transformants the transforming DNA appears to be present in the form of extrachromosomal elements.
Abstract: The Trichoderma reesei orotidine-5′-phosphate decarboxylase gene was isolated by heterologous hybridization with the corresponding Neurospora gene as a probe A 27 kb SalI fragment, which exclusively hybridized to the Neurospora gene, was subcloned in pGEM-5Zf(+) This subclone was termed pFG1 and was used to transform a Trichoderma reesei pyrG- negative mutant to PYR+ The transformation frequency in this homologous system was up to 12000 transformants per μg DNA About one-fifth of the transformants tested were abortive Perfect mitotic stability was found in half of the non-abortive transformants, correlating with vector integration at homologous and ectopic loci In the unstable transformants the transforming DNA appears to be present in the form of extrachromosomal elements

81 citations

Journal ArticleDOI
TL;DR: Northern (RNA) analysis showed that cel3 gene expression was induced by cellulose and repressed by glucose, fructose, 2-deoxyglucose, and lactose, which meant that Glycerol, mannitol, sorbitol, and maltose were neutral carbon sources.
Abstract: A 52-kDa protein, CEL3, has been separated from the culture filtrate of Agaricus bisporus during growth on cellulose. A PCR-derived probe was made, with a degenerate oligodeoxynucleotide derived from the amino acid sequence of a CEL3 CNBr cleavage product and was used to select cel3 cDNA clones from an A. bisporus cDNA library. Two allelic cDNAs were isolated. They showed 98.8% identity of their nucleotide sequences. The deduced amino acid sequence and domain architecture of CEL3 showed a high degree of similarity to those of cellobiohydrolase II of Trichoderma reesei. Functional expression of cel3 cDNA in Saccharomyces cerevisiae was achieved by placing it under the control of a constitutive promoter and fusing it to the yeast invertase signal sequence. Recombinant CEL3 secreted by yeast showed enzymatic activity towards crystalline cellulose. At long reaction times, CEL3 was also able to degrade carboxymethyl cellulose. Northern (RNA) analysis showed that cel3 gene expression was induced by cellulose and repressed by glucose, fructose, 2-deoxyglucose, and lactose. Glycerol, mannitol, sorbitol, and maltose were neutral carbon sources. Nuclear run-on analysis showed that the rate of synthesis of cel3 mRNA in cellulose-grown cultures was 13 times higher than that in glucose-grown cultures. A low basal rate of cel3 mRNA synthesis was observed in the nuclei isolated from glucose-grown mycelia.

81 citations

Journal ArticleDOI
TL;DR: Four new polyketide derivatives were isolated from the marine-derived fungus Trichoderma reesei, and the chemical structures and absolute configurations of compounds 1-4 were elucidated by extensive spectroscopic methods, especially 2D NMR and CD spectral analysis, and supported by their proposed biosynthesis pathway.

81 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202373
2022177
2021134
2020141
2019138
2018142