scispace - formally typeset
Search or ask a question
Topic

Trichoderma reesei

About: Trichoderma reesei is a research topic. Over the lifetime, 3832 publications have been published within this topic receiving 152877 citations. The topic is also known as: Trichoderma reesi.


Papers
More filters
Journal ArticleDOI
Y.H. Yang1, Bochu Wang1, Qiong Wang1, L.J. Xiang1, C.R Duan1 
TL;DR: In this paper, a microbial consortium of Trichoderma reesei AS3.3711, Aspergillus niger 3.316 and Saccharomyces cerevisiaes AS2.399 was constructed to decomposed rice chaff on the basis of the characters of each microorganism and the mechanism of cellulases.

63 citations

Journal ArticleDOI
TL;DR: Pure cellobiohydrolases I and II from Trichoderma reesei adsorb strongly (K=104M−1) onto micro-crystalline Avicel and synergistic effects are observed dependent on the ratio of the enzymes.
Abstract: Pure cellobiohydrolases I and II (CBH I & II) fromTrichoderma reesei adsorb strongly (K=104M−1) onto micro-crystalline Avicel. Equilibration is slow (>40 min) and saturation levels determined from the adsorption isotherms are almost identical:107–110 nmoles enzyme/mg Avicel. In admixture synergistic effects are observed dependent on the ratio of the enzymes. These effects are maximal for non-saturating conditions (1–10 μM) and when the enzymes are added in two consecutive steps synergism of binding is only apparent for CBH I.

63 citations

Journal ArticleDOI
TL;DR: In vivo footprinting analysis of xylan-induced and noninduced mycelia and electrophoretic mobility shift assay data support a model of xyn2 regulation based on the interplay of Hap2/3/5, Ace2 and the AGAA-box binding repressor.
Abstract: The xylanase system of the filamentous fungus Hypocrea jecorina (Trichoderma reesei) consists of two specific xylanases, Xyn1 and Xyn2, which are simultaneously expressed during growth on xylan but respond differentially to low-molecular-weight inducers. Using in vivo footprinting analysis of xylan-induced and noninduced mycelia, we detected two adjacent nucleotide sequences (5′-AGAA-3′ on the noncoding strand and 5′-GGGTAAATTGG-3′, referred to as the xylanase-activating element [XAE], on the coding strand, respectively) to bind proteins. Among these, binding to the AGAA-box is only observed under noninduced conditions, whereas binding to XAE is constitutive. Electrophoretic mobility shift assay with heterologously expressed components of the H. jecorina Hap2/3/5 protein complex and the cellulase regulator Ace2 suggests that these two transactivators form the protein complex binding to XAE. H. jecorina transformants, containing correspondingly mutated versions of the xyn2 promoter fused to the Aspergillus niger goxA gene as a reporter, revealed that the elimination of protein binding to the AGAA-box resulted in a threefold increase in both basal and induced transcription, whereas elimination of Ace2 binding to its target in XAE completely eliminated transcription under both conditions. Destruction of the CCAAT-box by insertion of a point mutation prevents binding of the Hap2/3/5 complex in vitro and results in a slight increase in both basal and induced transcription. These data support a model of xyn2 regulation based on the interplay of Hap2/3/5, Ace2 and the AGAA-box binding repressor.

63 citations

01 Jan 2000
TL;DR: In this paper, the cellulase production of a filamentous fungi, Trichoderma reesei Rut C 30, was examined on carbon sources obtained after steam pretreatment of spruce.
Abstract: Various techniques are available for the conversion of lignocellulosics to fuel ethanol. During the last decade processes based on enzymatic hydroly­ sis of cellulose have been investigated more extensively, showing good yield on both hardwood and softwood. The cellulase production of a filamentous fungi, Trichoderma reesei Rut C 30, was examined on carbon sources obtained after steam pretreatment of spruce. These materials were washed fibrous steam-pretreated spruce (SPS), and hemicellulose hydrolysate. The hemicel­ lulose hydrolysate contained, besides water-soluble carbohydrates, lignin and sugar degradation products, which were formed during the pretreat­ ment and proved to be inhibitory to microorganisms. Experiments were performed in a 4-L laboratory fermentor. The hydrolytic capacity of the pro­ duced enzyme solutions was compared with two commercially available enzyme preparations, Celluclast and Iogen Cellulase, on SPS, washed SPS, and Solka Floc cellulose powder. There was no significant difference among the different enzymes produced by T. reesei Rut C 30. However, the conver­ sion of cellulose using these enzymes was higher than that obtained with Iogen or Celluclast cellulases using steam-pretreated spruce as substrate. Index Entries: Cellulase production; steam-pretreated spruce; enzymatic capacity of cellulases; filter paper activity measurement; Trichoderma.

63 citations

Journal ArticleDOI
TL;DR: It is found that an appropriate extracellular Ca2+ concentration markedly stimulated the hyphal growth, cellulase production, and total protein secretion of the cellulase hyper‐producing strain, Trichoderma reesei Rut‐C30.
Abstract: Calcium signaling plays pivotal roles in the hyphal growth, conidiation, and osmosis sensitivity of fungi through the Ca(2+) /calmodulin-calcineurin-dependent pathway. This study found that an appropriate extracellular Ca(2+) concentration markedly stimulated the hyphal growth, cellulase production, and total protein secretion of the cellulase hyper-producing strain, Trichoderma reesei Rut-C30. Transcription analysis revealed upregulation of not only encoding genes of cellulases and the transcriptional activator XYR1 but also several genes encoding endoplasmic reticulum-chaperones after Ca(2+) addition. The function of CRZ1, T. reesei calcineurin-responsive zinc finger transcription factor 1, was further characterized by gene disruption. Electrophoretic mobility shift assays (EMSAs) in combination with chromatin immunoprecipitation (ChIP) verified that CRZ1 could bind directly to the upstream regions of xyr1 and cbh1 (cellobiohydrolase I-encoding gene) in response to Ca(2+) . A DNase I footprinting assay identified its putative binding consensus site (5'-[T/G]GGCG-3' or 5'-GGGC[G/T]-3'). EMSAs confirmed that CRZ1 competed for occupancy of the xyr1 promoter with another transcription factor, ACE1. These results revealed putative signaling pathways downstream of calcineurin in response to extracellular Ca(2+) involved in upregulation of cellulose degradation-related genes, reflecting progress in the study of Ca(2+) signaling in filamentous fungi. This study also provides insight that will facilitate further improvement of (hemi-)cellulase production by T. reesei.

63 citations


Network Information
Related Topics (5)
Fermentation
68.8K papers, 1.2M citations
87% related
Yeast
31.7K papers, 868.9K citations
85% related
Saccharomyces cerevisiae
32.1K papers, 1.6M citations
84% related
Escherichia coli
59K papers, 2M citations
83% related
Lignin
18.3K papers, 659.8K citations
82% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202373
2022177
2021134
2020141
2019138
2018142