Topic
Trichoderma reesei
About: Trichoderma reesei is a research topic. Over the lifetime, 3832 publications have been published within this topic receiving 152877 citations. The topic is also known as: Trichoderma reesi.
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TL;DR: The complete protein pattern of cellulase and hemicellulase genes was studied through the Genome-wide analysis in Trichoderma reesei and four groups were created and analyzed by phylogenetic analysis, in which 8 beta-glucosidases appeared to be non-secreted proteins.
Abstract: The complete protein pattern of cellulase and hemicellulase genes was studied through the Genome-wide analysis in Trichoderma reesei. The genome database revealed the presence of 39 ORFs encoding related proteins, including 32 enzymes with a catalysis domain related to cellulases and hemicellulases and 7 related proteins with a cellulose-binding module (CBM). Ten of these encoded yet undescribed enzymes, including six novel beta-glucosidases or xylosidases, two putative xylanases and two undescribed mannases. To better illustrate the relation of these 39 related proteins, four groups were created and analyzed by phylogenetic analysis: group A corresponding to xylanases, group B belonging to mannases and acting to degrade mannan; group C containing all known and putative cellulose-degrading proteins that have highly conserved CBMs; and group D containing beta-glucosidase and beta-xylosidase. Group D was the largest group, in which 8 beta-glucosidases appeared to be non-secreted proteins.
59 citations
TL;DR: The development of minimal enzyme cocktails for hydrolysis of Norway spruce and sugarcane bagasse, which were pretreated using the so-called BALI™ process, which is based on sulfite pulping technology are presented.
59 citations
TL;DR: The onset of resistance is similar for both the xylanase and acetyl esterase enzymes, and both enzymes were completely inhibited by a degree of acetylation of 1.5 moles acetyl groups per mole anhydroxylose units at all enzyme loadings.
Abstract: The effect of the degree of acetylation on the enzymatic digestibility of acetylated xylans has been investigated. Oatspelts xylans were reacetylated to degrees of 0.26 to 1.67 moles acetyl groups per mole of anhydroxylose units. These acetylated samples were then used to study the effect of acetylation on the xylanase (EC 3.2.1.8) and acetyl esterase (EC 3.1.1.6) activities of a commercial Trichoderma reesei cellulase preparation. The enzymatic digestibility was dramatically affected by the degree of acetylation. The onset of resistance is similar for both the xylanase and acetyl esterase enzymes, and both enzymes were completely inhibited by a degree of acetylation of 1.5 moles acetyl groups per mole anhydroxylose units at all enzyme loadings.
59 citations
TL;DR: Significant improvements in the reducing sugar and glucose yields were observed in the hydrolysate of EBI pretreated biomass compared to the control and a significant negative correlation was observed between degree of crystallinity and the glucose yield from enzymatic hydrolysis.
59 citations
TL;DR: In this paper, the authors characterized the activities of TC, EG-rich, and CBH-rich cellulases from T reesei and have shown that their activities towards cotton fabrics are influenced by ionic strength and adsorbed ionic species as well as by temperature and pH Adsorption and kinetic experiments confirm that increasing mechanical agitation favors EG attack by greatly increasing the availability of sites for EG adsorption.
Abstract: We have characterized the activities of TC, EG-rich, and CBH-rich cellulases from T reesei and have shown that their activities towards cotton fabrics are influenced by ionic strength and adsorbed ionic species as well as by temperature and pH Adsorption and kinetic experiments confirm that increasing mechanical agitation favors EG attack by greatly increasing the availability of sites for EG adsorption It is not clear whether this is a consequence of fiber fibrillation damage or of improved access to fiber surfaces deep within the fabric structure The enhanced rate of cellulolytic hydrolysis of mer cerized cotton and the inhibitory effects on reactive and direct dyed fabrics are ex plained mainly in terms of increased or reduced availability of adsorption sites for CBHS and EGS The implications for textile finishing are far-reaching It is clear that a fabric's processing history (especially mercerizing and dyeing), construction, and level of ap plied mechanical agitation can be as important as the
59 citations