Topic
Trichoderma reesei
About: Trichoderma reesei is a research topic. Over the lifetime, 3832 publications have been published within this topic receiving 152877 citations. The topic is also known as: Trichoderma reesi.
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TL;DR: The objectives of the present study were to continue the investigation of the regulation of cellulase production in Trichoderma reesei by fermentation conditions, to compare the responses of the parent strain to those of the mutants, and to identify the inducer in the hydrolysis syrups.
Abstract: The objectives of the present study were to continue our investigation of the regulation of cellulase production in Trichoderma reesei by fermentation conditions, to compare the responses of the parent strain to those of the mutants, and to identify the inducer in the hydrolysis syrups
48 citations
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TL;DR: Results show that replacing natural transcription factors with minimal transcriptional activators is a powerful strategy to enhance cellulase production in T. reesei.
Abstract: Cellulase can convert lignocellulosic feedstocks into fermentable sugars, which can be used for the industrial production of biofuels and chemicals. The high cost of cellulase production remains a challenge for lignocellulose breakdown. Trichoderma reesei RUT C30 serves as a well-known industrial workhorse for cellulase production. Therefore, the enhancement of cellulase production by T. reesei RUT C30 is of great importance. Two sets of novel minimal transcriptional activators (DBDace2-VP16 and DBDcre1-VP16) were designed and expressed in T. reesei RUT C30. Expression of DBDace2-VP16 and DBDcre1-VP16 improved cellulase production under induction (avicel or lactose) and repression (glucose) conditions, respectively. The strain TMTA66 under avicel and TMTA139 under glucose with the highest cellulase activities outperformed other transformants and the parental strain under the corresponding conditions. For TMTA66 strains, the highest FPase activity was approximately 1.3-fold greater than that of the parental strain RUT C30 at 120 h of cultivation in a shake flask using avicel as the sole carbon source. The FPase activity (U/mg biomass) in TMTA139 strains was approximately 26.5-fold higher than that of the parental strain RUT C30 at 72 h of cultivation in a shake flask using glucose as the sole carbon source. Furthermore, the crude enzymes produced in the 7-L fermenter from TMTA66 and TMTA139 supplemented with commercial β-glucosidase hydrolyzed pretreated corn stover effectively. These results show that replacing natural transcription factors with minimal transcriptional activators is a powerful strategy to enhance cellulase production in T. reesei. Our current study also offers an alternative genetic engineering strategy for the enhanced production of industrial products by other fungi.
48 citations
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TL;DR: The CEC degraded xylan, mannose-based substrates, β-1,4-linked glucans, including microcrystalline cellulose (Avicel), and non-pretreated timothy grass and rice straw, however, degradation of chitin, pectin, dextran, and wheat starch was not observed.
48 citations
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TL;DR: This work reports the isolation of a constitutively cellulase producing mutant in T. reesei that produces high levels of all of the component enzymes in the cellulase complex in the presence of glucose or sucrose.
Abstract: The single major factor limiting the economy of enzymatic hydrolysis of cellulose is the cost of the cellulase enzymes. Most of the work to date in reducing this cost has centered around selection of hyperproducing strains using the standard cellulose medium. Cellulose is an insoluble substrate with obvious design disadvantages in bioreactors. It is often indicated that another reasonable approach to the problem would be the isolation of constitutive mutants which require no inducer to form cellulase. In fact, the goal of several laboratories around the world working on the hydrolysis of cellulosic substances has been to isolate a constitutive mutant. Reported here is the isolation of a constitutively cellulase producing mutant in T. reesei that produces high levels of all of the component enzymes in the cellulase complex in the presence of glucose or sucrose. With this constitutive mutant, it is hoped that a major hurdle of working with an insoluble substrate will be overcome. The mutant will allow easier operation and control of cellulose bioreactors and can be used for continuous enzyme production, as opposed to the present batch production system. Also, a number of other unusable susbtrates like whey and molasses can be used for commercial preparationsmore » of cellulases. (Refs. 2).« less
48 citations
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TL;DR: Results indicate that cellulose‐digesting enzymes enhance the release of sperm and epithelial cells from a cotton swab over buffer alone, providing for efficient DNA analysis.
Abstract: This report describes development of a method for enhanced cell elution from cotton swabs. The method exploits an enzyme mixture for digestion of the cotton to remove intact cells, and can be utilized in conjunction with or to circumvent conventional differential extraction (DE). Samples digested with Aspergillus niger cellulase yielded sperm cell recoveries (18 ± 3.5%) similar to conventional DE buffer (23 ± 7.8%) while providing intact epithelial cells. Storage time affected the concentration of enzyme required for optimal sperm cell recovery, with longer times requiring increased cellulase concentrations. Cellulase from A. niger yielded a twofold enhancement in sperm cell elution over buffer alone, and preliminary testing of higher activity cellulases from Trichoderma reesei and Trichoderma viride showed even greater enhancement. These results indicate that cellulose-digesting enzymes enhance the release of sperm and epithelial cells from a cotton swab over buffer alone, providing for efficient DNA analysis.
48 citations