Topic
Trichoderma reesei
About: Trichoderma reesei is a research topic. Over the lifetime, 3832 publications have been published within this topic receiving 152877 citations. The topic is also known as: Trichoderma reesi.
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TL;DR: The hypothesis that both surfaces of the CBD are involved in the interaction of the binding domain with crystalline cellulose is supported, suggesting that the interaction with the pyranose ring of the substrate with an aromatic side chain is important.
Abstract: The function of the cellulose-binding domain (CBD) of the cellobiohydrolase I of Trichoderma reesei was studied by site-directed mutagenesis of two amino acid residues identified by analyzing the 3D structure of this domain. The mutant enzymes were produced in yeast and tested for binding and activity on crystalline cellulose. Mutagenesis of the tyrosine residue (Y492) located at the tip of the wedge-shaped domain to alanine or aspartate reduced the binding and activity on crystalline cellulose to the level of the core protein lacking the CBD. However, there was no effect on the activity toward small oligosaccharide (4-methylumbelliferyl beta-D-lactoside). The mutation tyrosine to histidine (Y492H) lowered but did not destroy the cellulose binding, suggesting that the interaction of the pyranose ring of the substrate with an aromatic side chain is important. However, the catalytic activity of this mutant on crystalline cellulose was identical to the other two mutants. The mutation P477R on the edge of the other face of the domain reduces both binding and activity of CBHI. These results support the hypothesis that both surfaces of the CBD are involved in the interaction of the binding domain with crystalline cellulose.
203 citations
TL;DR: The degradation of organic substrates were quick (within 9-12 days) in case of rapid composting as indicated by the reduction (below 20) in C/N ratio, whereas, normal composting took more than 20 days to attain C/n ratio of below 20.
203 citations
TL;DR: It is evident that the presence of a functional CBD is increasingly important for CBH I toward higher enzyme to cellulose ratios and the putative hinge removed by the first deletion facilitates CBD-driven binding and dense packing of the wild type enzyme on the cellulose surface.
202 citations
TL;DR: It is shown by hybrid messenger selection and partial DNA sequencing that one of the clones contains a gene coding for the major cellulolytic enzyme of T. reesei, cellobiohydrolase I.
Abstract: The filamentous fungus Trichoderma reesei produces a number of inducible cellulolytic enzymes. By differential hybridization of cDNA probes made from induced and repressed messenger RNA populations to a λ phage gene bank, we have isolated a number of genes strongly expressed during production of cellulolytic enzymes. We have shown by hybrid messenger selection and partial DNA sequencing that one of the clones contains a gene coding for the major cellulolytic enzyme of T. reesei, cellobiohydrolase I.
200 citations
TL;DR: Trichoderma reesei and Aspergillus awamori enzymes were concentrated, pooled and assessed for the hydrolysis of steam-pretreated sugarcane bagasse as discussed by the authors.
200 citations