Topic
Trichoderma reesei
About: Trichoderma reesei is a research topic. Over the lifetime, 3832 publications have been published within this topic receiving 152877 citations. The topic is also known as: Trichoderma reesi.
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TL;DR: A nitrogen analyzer was used to directly estimate the amount of enzyme adsorbed on biomass solids, rather than relying on the difference between initial and final dissolved protein as typically practiced, and revealed that sugars and especially cellobiose strongly inhibit cellulase binding, and cellulases binding was completely shut down at cellobioses concentrations greater than 150 g/L.
118 citations
TL;DR: A major beta-glucosidase I and a minor beta-greusidase II were purified from culture filtrates of the fungus Trichoderma reesei grown on wheat straw using CM-Sepharose CL-6B cation exchange and DEAE Bio-Gel A anion-exchange chromatography steps as mentioned in this paper.
117 citations
TL;DR: Corn fiber, currently produced at wet milling facilities, is readily available as a potential feedstock for production of fermentable sugars and monosaccharide sugar yields obtained using the blended preparations were 74 and 54% of the available arabinose and xylose, respectively.
117 citations
TL;DR: The data show that the biological roles of A. nidulans LaeA and T. reesei LAE1 are much less conserved than hitherto thought and appears predominantly to regulate genes increasing relative fitness in its environment.
Abstract: The putative methyltransferase LaeA is a global regulator that affects the expression of multiple secondary metabolite gene clusters in several fungi, and it can modify heterochromatin structure in Aspergillus nidulans. We have recently shown that the LaeA ortholog of Trichoderma reesei (LAE1), a fungus that is an industrial producer of cellulase and hemicellulase enzymes, regulates the expression of cellulases and polysaccharide hydrolases. To learn more about the function of LAE1 in T. reesei, we assessed the effect of deletion and overexpression of lae1 on genome-wide gene expression. We found that in addition to positively regulating 7 of 17 polyketide or nonribosomal peptide synthases, genes encoding ankyrin-proteins, iron uptake, heterokaryon incompatibility proteins, PTH11-receptors, and oxidases/monoxygenases are major gene categories also regulated by LAE1. chromatin immunoprecipitation sequencing with antibodies against histone modifications known to be associated with transcriptionally active (H3K4me2 and -me3) or silent (H3K9me3) chromatin detected 4089 genes bearing one or more of these methylation marks, of which 75 exhibited a correlation between either H3K4me2 or H3K4me3 and regulation by LAE1. Transformation of a laeA-null mutant of A. nidulans with the T. reesei lae1 gene did not rescue sterigmatocystin formation and further impaired sexual development. LAE1 did not interact with A. nidulans VeA in yeast two-hybrid assays, whereas it interacted with the T. reesei VeA ortholog, VEL1. LAE1 was shown to be required for the expression of vel1, whereas the orthologs of velB and VosA are unaffected by lae1 deletion. Our data show that the biological roles of A. nidulans LaeA and T. reesei LAE1 are much less conserved than hitherto thought. In T. reesei, LAE1 appears predominantly to regulate genes increasing relative fitness in its environment.
117 citations
TL;DR: The biomass-deconstructing capability of Streptomyces sp.
Abstract: Streptomyces are best known for producing antimicrobial secondary metabolites, but they are also recognized for their contributions to biomass utilization. Despite their importance to carbon cycling in terrestrial ecosystems, our understanding of the cellulolytic ability of Streptomyces is currently limited to a few soil-isolates. Here, we demonstrate the biomass-deconstructing capability of Streptomyces sp. SirexAA-E (ActE), an aerobic bacterium associated with the invasive pine-boring woodwasp Sirex noctilio. When grown on plant biomass, ActE secretes a suite of enzymes including endo- and exo-cellulases, CBM33 polysaccharide-monooxygenases and hemicellulases. Genome-wide transcriptomic and proteomic analyses and biochemical assays have revealed the key enzymes used to deconstruct crystalline cellulose, other pure polysaccharides and biomass. The mixture of enzymes obtained from growth on biomass has biomass-degrading activity comparable to a cellulolytic enzyme cocktail from the fungus Trichoderma reesei and thus provides a compelling example of high cellulolytic capacity in an aerobic bacterium.
117 citations