Topic
Trichoderma reesei
About: Trichoderma reesei is a research topic. Over the lifetime, 3832 publications have been published within this topic receiving 152877 citations. The topic is also known as: Trichoderma reesi.
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TL;DR: The T atroviride mutants produced high levels of extracellular cellulases as well as beta-glucosidase, rendering the need for beta- GLUCOSidase supplementation in hydrolysis of cellulose or pretreated willow unnecessary.
96 citations
TL;DR: It is found that both ACY1 and PKA catalytic subunit 1 (PKAC1) are involved in regulation of vegetative growth but are not essential for sexual development and analysis of transcript levels of cellulase regulator genes indicates that the regulatory output of the cAMP pathway may be established via adjustment of XYR1 abundance.
Abstract: The cyclic AMP (cAMP) pathway represents a central signaling cascade with crucial functions in all organisms. Previous studies of Trichoderma reesei (anamorph of Hypocrea jecorina) suggested a function of cAMP signaling in regulation of cellulase gene expression. We were therefore interested in how the crucial components of this pathway, adenylate cyclase (ACY1) and cAMP-dependent protein kinase A (PKA), would affect cellulase gene expression. We found that both ACY1 and PKA catalytic subunit 1 (PKAC1) are involved in regulation of vegetative growth but are not essential for sexual development. Interestingly, our results showed considerably increased transcript abundance of cellulase genes in darkness compared to light (light responsiveness) upon growth on lactose. This effect is strongly enhanced in mutant strains lacking PKAC1 or ACY1. Comparison to the wild type showed that ACY1 has a consistently positive effect on cellulase gene expression in light and darkness, while PKAC1 influences transcript levels of cellulase genes positively in light but negatively in darkness. A function of PKAC1 in light-modulated cellulase gene regulation is also reflected by altered complex formation within the cel6a/cbh2 promoter in light and darkness and in the absence of pkac1. Analysis of transcript levels of cellulase regulator genes indicates that the regulatory output of the cAMP pathway may be established via adjustment of XYR1 abundance. Consequently, both adenylate cyclase and protein kinase A are involved in light-modulated cellulase gene expression in T. reesei and have a dampening effect on the light responsiveness of this process.
96 citations
TL;DR: A cellulase hyperproducing mutant, whose enzyme is more resistant to catabolite repression by glucose and cellobiose, was isolated and enhancement in cellulase yields and hydrolytic potential was achieved.
96 citations
TL;DR: The results suggest that the Cel7A linker is an intrinsically disordered protein with and without glycosylation, and indicates that linkers from Family 7 enzymes from other genera and other cellulases within T. reesei may not be as disordered, warranting further study.
95 citations
TL;DR: Purified cellulase EG II is most effective at removing color from denim, producing a good stone washing effect with the lowest hydrolysis level, but the experimental cellulase mixture Cellulase B removes color only with the highest enzyme dosage.
Abstract: Cellulases are widely used to age denim fabrics. In this work the effects of three purified monocomponent cellulases, EG I, EG II, and CBH I, and two different cellulase mixtures produced by genetically modified strains of Trichoderma reesei are compared for stone washing denim fabrics. Stone washing effects are evaluated by analyzing the soluble reducing sugars, absorbance, and lightness values of the treatment solutions. The prop erties of denim swatches are evaluated by reflectance units and by a panel. Purified cellulase EG II is most effective at removing color from denim, producing a good stone washing effect with the lowest hydrolysis level. Treatment with CBH I does not produce any stone washing effect, even at high enzyme dosages. The commercial cellulase Ecostone® L produces a good stone washing effect, but the experimental cellulase mixture Cellulase B removes color only with the highest enzyme dosage, i.e., when the amount of EGS in the mixture is high enough.
95 citations