Topic
Trichoderma reesei
About: Trichoderma reesei is a research topic. Over the lifetime, 3832 publications have been published within this topic receiving 152877 citations. The topic is also known as: Trichoderma reesi.
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TL;DR: The results suggested that co-fungi cultivation was a valuable technique for corn stover bioconversion, which could produce high efficiency of lignocellulolytic enzyme system as a cheaper alternative to commercial enzymes for industrial utilization.
88 citations
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TL;DR: An overview of new findings in the gene expression regulation network of T. reesei is presented and how synthetic biology strategies can be used to create engineered promoters to efficiently synthesize enzymes for biomass degradation to produce bioethanol is presented.
88 citations
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TL;DR: It was shown that production of this xylanase was clearly increased when it was produced as a fusion protein with a carrier polypeptide having an intact domain structure compared to the production of fusions to the signal sequence alone or to carriers having incomplete domain structures.
Abstract: A bacterial xylanase gene, Nonomuraea flexuosa xyn11A, was expressed in the filamentous fungus Trichoderma reesei from the strong cellobiohydrolase 1 promoter as fusions to a variety of carrier polypeptides By using single-copy isogenic transformants, it was shown that production of this xylanase was clearly increased (up to 820 mg/liter) when it was produced as a fusion protein with a carrier polypeptide having an intact domain structure compared to the production (150 to 300 mg/liter) of fusions to the signal sequence alone or to carriers having incomplete domain structures The carriers tested were the T reesei mannanase I (Man5A, or MANI) core-hinge and a fragment thereof and the cellulose binding domain of T reesei cellobiohydrolase II (Cel6A, or CBHII) with and without the hinge region(s) and a fragment thereof The flexible hinge region was shown to have a positive effect on both the production of Xyn11A and the efficiency of cleavage of the fusion polypeptide The recombinant Xyn11A produced had properties similar to those of the native xylanase It constituted 6 to 10% of the total proteins secreted by the transformants About three times more of the Man5A core-hinge carrier polypeptide than of the recombinant Xyn11A was observed Even in the best Xyn11A producers, the levels of the fusion mRNAs were only approximately 10% of the level of cel7A (cbh1) mRNA in the untransformed host strain
88 citations
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TL;DR: These results demonstrate that the corn stover-adapted microbial consortium EMSD5 harbors a variety of lignocellulolytic anaerobic bacteria and degradative enzymes, especially those implicated in hemicellulose decomposition.
Abstract: Microbial consortia represent promising candidates for aiding in the development of plant biomass conversion strategies for biofuel production. However, the interaction between different community members and the dynamics of enzyme complements during the lignocellulose deconstruction process remain poorly understood. We present here a comprehensive study on the community structure and enzyme systems of a lignocellulolytic microbial consortium EMSD5 during growth on corn stover, using metagenome sequencing in combination with quantitative metaproteomics. The taxonomic affiliation of the metagenomic data showed that EMSD5 was primarily composed of members from the phyla Proteobacteria, Firmicutes and Bacteroidetes. The carbohydrate-active enzyme (CAZyme) annotation revealed that representatives of Firmicutes encoded a broad array of enzymes responsible for hemicellulose and cellulose deconstruction. Extracellular metaproteome analysis further pinpointed the specific role and synergistic interaction of Firmicutes populations in plant polysaccharide breakdown. In particular, a wide range of xylan degradation-related enzymes, including xylanases, β-xylosidases, α-l-arabinofuranosidases, α-glucuronidases and acetyl xylan esterases, were secreted by diverse members from Firmicutes during growth on corn stover. Using label-free quantitative proteomics, we identified the differential secretion pattern of a core subset of enzymes, including xylanases and cellulases with multiple carbohydrate-binding modules (CBMs). In addition, analysis of the coordinate expression patterns indicated that transport proteins and hypothetical proteins may play a role in bacteria processing lignocellulose. Moreover, enzyme preparation from EMSD5 demonstrated synergistic activities in the hydrolysis of pretreated corn stover by commercial cellulases from Trichoderma reesei. These results demonstrate that the corn stover-adapted microbial consortium EMSD5 harbors a variety of lignocellulolytic anaerobic bacteria and degradative enzymes, especially those implicated in hemicellulose decomposition. The data in this study highlight the pivotal role and cooperative relationship of Firmicutes members in the biodegradation of plant lignocellulose by EMSD5. The differential expression patterns of enzymes reveal the strategy of sequential lignocellulose deconstruction by EMSD5. Our findings provide insights into the mechanism by which consortium members orchestrate their array of enzymes to degrade complex lignocellulosic biomass.
88 citations
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TL;DR: The results suggest that HFBI has a role in hyphal development and HFBII in sporulation, and Expressing the Schizophyllum commune hydrophobin SC3 in the Deltahfb1 strain restored the formation of aerial hyphae.
Abstract: Hydrophobins are fungal self-assembling proteins. Here, the hydrophobin genes hfb1 and hfb2 were deleted in Trichoderma reesei and their biological roles studied. Our results suggest that HFBI has a role in hyphal development and HFBII in sporulation. Sporulating colonies of the Deltahfb2 strain were wettable and sporulation was only 50% of the parent strain. Colonies of Deltahfb1 showed wettable and fluffy phenotype. In shaken liquid cultures, the hyphae of Deltahfb1 were thinner and biomass formation was slower compared to the parent strain while in static liquid cultures no aerial hyphae were formed. Expressing the Schizophyllum commune hydrophobin SC3 in the Deltahfb1 strain restored the formation of aerial hyphae.
87 citations