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Showing papers on "Triterpene published in 2007"


Journal ArticleDOI
TL;DR: The oleanane skeleton was the most common skeleton and is present in most orders of the plant kingdom, and the relationship between the type of skeleton and the plant origin was investigated.

592 citations


Journal ArticleDOI
TL;DR: Nine lucidenic acids and four ganoderic acids were found to inhibit TPA‐induced inflammation in mice, and 20‐hydroxylucidenic acid N exhibited inhibitory effects on skin‐tumor promotion in an in vivo two‐stage mouse‐skin carcinogenesis test based on 7,12‐dimethylbenz[a]anthracene as initiator, and with TPA as promoter.
Abstract: A series of lanostane-type triterpene acids, including eleven lucidenic acids (3, 4, 9, 10, 13-19) and six ganoderic acids (20-22, 24, 26, 27), as well as six sterols (28-33), all isolated from the fruiting bodies of the fungus Ganoderma lucidum, were examined for their inhibitory effects on the induction of Epstein-Barr virus early antigen (EBV-EA) by 12-O-tetradecanoylphorbol-13-acetate (TPA) in Raji cells, a known primary screening test for anti-tumor promoters. All of the compounds tested, except for ganolactone (27) and three sterols (29-31), showed potent inhibitory effects on EBV-EA induction, with IC(50) values of 235-370 mol ratio/32 pmol TPA. In addition, nine lucidenic acids (1, 2, 5-8, 11, 12, 18) and four ganoderic acids (20, 23-25) were found to inhibit TPA-induced inflammation (1 microg/ear) in mice, with ID(50) values of 0.07-0.39 mg per ear. Further, 20-hydroxylucidenic acid N (18) exhibited inhibitory effects on skin-tumor promotion in an in vivo two-stage mouse-skin carcinogenesis test based on 7,12-dimethylbenz[a]anthracene (DMBA) as initiator, and with TPA as promoter.

180 citations


Journal ArticleDOI
TL;DR: In this paper, three acylated oleanane-type triterpene saponins, theasaponins A6 (1), A7 (2), and B5 (3), were isolated from the saponin fraction of the seeds of the Japanese tea plant Camellia sinensis together with the known constituent foliatheasaponin III.
Abstract: Three new acylated oleanane-type triterpene saponins, theasaponins A6 (1), A7 (2), and B5 (3), were isolated from the saponin fraction of the seeds of the Japanese tea plant Camellia sinensis together with the known constituent foliatheasaponin III (4). The structures of the glycosides 1–3 were elucidated on the basis of spectroscopic, chemical, and physico-chemical evidence.

132 citations


Journal ArticleDOI
TL;DR: Thirteen cucurbitane-type triterpene glycosides, including eight new compounds named charantosides, were isolated from a methanol extract of the fruits of Japanese Momordica charantia and showed inhibitory effects on EBV-EA induction with IC(50) values of 200-409 mol ratio/32 pmol TPA.
Abstract: Thirteen cucurbitane-type triterpene glycosides, including eight new compounds named charantosides I (6), II (7), III (10), IV (11), V (12), VI (13), VII (16), and VIII (17), and five known compounds, 8, 9, 14, 15, and 18, were isolated from a methanol extract of the fruits of Japanese Momordica charantia. The structures of the new compounds were determined on the basis of spectroscopic methods. On evaluation of these triterpene glycosides and five other cucurbitane-type triterpenes, 1-5, also isolated from the extract of M. charantia fruits, for their inhibitory effects on the induction of Epstein-Barr virus early antigen (EBV-EA) by 12-O-tetradecanoylphorbol-13-acetate (TPA) in Raji cells, these compounds showed inhibitory effects on EBV-EA induction with IC(50) values of 200-409 mol ratio/32 pmol TPA. In addition, upon evaluation of compounds 1-5 for inhibitory effects against activation of (+/-)-(E)-methyl-2[(E)-hydroxyimino]-5-nitro-6-methoxy-3-hexemide (NOR 1), a nitrogen oxide (NO) donor, compounds 1-3 showed moderate inhibitory effects. Compounds 1 and 2 exhibited marked inhibitory effects in both 7,12-dimethylbenz[a]anthracene (DMBA)- and peroxynitrite (ONOO-; PN)-induced mouse skin carcinogenesis tests.

126 citations


Journal ArticleDOI
TL;DR: Six new triterpenes and four known compounds have been isolated from dried stems and leaves of Melissa officinalis and the structures of compounds 1- 6 were established by analysis of spectroscopic data.
Abstract: Six new triterpenes (1–6) and four known compounds have been isolated from dried stems and leaves of Melissa officinalis. The known compounds were identified as quadranoside III, salvianic acid A, rosmarinic acid, and luteolin. The structures of compounds 1–6 were established by analysis of spectroscopic data. Free radical scavenging and antimicrobial activities of the extracts and of rosmarinic acid, the major component, were evaluated.

116 citations


Journal ArticleDOI
TL;DR: It is confirmed that betulinic acid saponins are generally better in vitro anticancer agents than those derived from betulin with the exception of betulin 3beta-O-alpha-d-mannopyranoside which exerted a potent cytotoxic activity against lung carcinoma and colorectal adenocarcinoma human cell lines with IC(50) ranging from 7.3 to 10.1mumol/L.

102 citations


Journal ArticleDOI
TL;DR: A comparative study on the antitrypanosomal activity of the isolated triterpenoids and sterols and some related compounds has indicated that the presence of an oxygenated function at C-28 or an oxygenations of the side chain atC-17 seems to be important for the antitriespanosome activity of triter penoid and sterol.
Abstract: Fractionation of an antitrypanosomal lipophilic leaf extract from Strychnos spinosa led to the isolation of eight triterpenoids and sterols in this plant part for the first time. Two of these were found to possess in vitro antitrypanosomal activity, namely, saringosterol (14) and 24-hydroperoxy-24-vinylcholesterol (15), with IC(50) values of 7.8 +/- 1.2 and 3.2 +/- 1.2 microM, respectively. The latter compound was isolated from a plant source for the first time. A comparative study on the antitrypanosomal activity of the isolated triterpenoids and sterols and some related compounds has indicated that the presence of an oxygenated function at C-28 or an oxygenated side chain at C-17 seems to be important for the antitrypanosomal activity of triterpenoids and sterols, respectively.

90 citations


Journal ArticleDOI
TL;DR: Six new lanostane-type triterpene acids isolated from the epidermis of the sclerotia of Poria cocos exhibited inhibitory effects on skin tumor promotion in an in vivo two-stage carcinogenesis test using 7,12-dimethylbenz[a]anthracene (DMBA) as an initiator and TPA as a promoter.
Abstract: The structures of six new lanostane-type triterpene acids isolated from the epidermis of the sclerotia of Poria cocos were established to be 15α-hydroxydehydrotumulosic acid (5), 16α,25-dihydroxyde...

87 citations


Journal ArticleDOI
TL;DR: In addition to fatty acids, a mixture of sterols, lupeol, arctigenin methylether, sesamin, vanillic acid, 2,6-dimethoxy-1,4-benzoquinone, betulinic acid and two pentacyclic triterpene acetates were isolated from Fagara tessmannii Engl.

81 citations


Journal ArticleDOI
TL;DR: Major product of OEA is α-amyrin (ursane skeleton), showing good accordance to higher content of ursane-type triterpene acids in the cultured cells, and strongly suggesting OEA to be a major contributor OSC for their production.
Abstract: Olive (Olea europaea) contains large quantity of triterpene acids including oleanolic acid (6) as a major one. Varieties of biological activities exhibited by triterpene acids attracted our attentions, especially from pharmaceutical viewpoints. Cell culture of olive plant was induced and its triterpene constituents were studied. From the cell suspension cultures, six ursane type triterpene acids; ursolic acid (9), pomolic acid (10), rotundic acid (11), tormentic acid (12), 2α-hydroxyursolic acid (13) and 19α-hydroxyasiatic acid (14), and two oleanane type acids; oleanolic acid and maslinic acid (7), have been isolated. Quantity of ursane type triterpene acids produced by cell cultures was larger than that of oleanane type. Further, a multifunctional oxidosqualene cyclase (OSC) named OEA was cloned by homology based PCRs from the same cultured cells. Major product of OEA is α-amyrin (ursane skeleton), showing good accordance to higher content of ursane-type triterpene acids in the cultured cells, and strongly suggesting OEA to be a major contributor OSC for their production.

79 citations


Journal ArticleDOI
TL;DR: Two new cucurbitane-type triterpene glycosides, colocynthosides A and B, were isolated together with 17 known constituents and elucidated on the basis of chemical and physicochemical evidence.
Abstract: The methanolic extract from the fruit of Citrullus colocynthis showed an inhibitory effect on ear passive cutaneous anaphylaxis reactions as a type I allergic model in mice From the methanolic extract, two new cucurbitane-type triterpene glycosides, colocynthosides A and B, were isolated together with 17 known constituents The structures of colocynthosides A and B were elucidated on the basis of chemical and physicochemical evidence In addition, the principal cucurbitane-type triterpene glycoside, cucurbitacin E 2-O-β-D-glucopyranoside, and its aglycon, cucurbitacin E, exhibited the antiallergic activity at a dose of 100 and 125 mg/kg, po, respectively

Journal ArticleDOI
TL;DR: The chromatographic behaviour of steroidal saponins found in Anemarrhena asphodeloides, Asparagus officinalis, Convallaria majalis, Digitalis purpurea and Ruscus aculeatus was studied and it is recommended that initial analysis of Saponins by HPLC-MS using a C-18 stationary phase is performed using acidified aqueous acetonitrile mobile phase gradients.

Journal ArticleDOI
TL;DR: It is concluded that acylation with angeloyl group at C21 and C22 positions of triterpenoid saponin is essential for its activity.

Journal ArticleDOI
TL;DR: It is suggested that both hydroxy and carboxy groups present in the triterpenes are important for their antibacterial activity against oral pathogens.
Abstract: Triterpene acids (ursolic, oleanoic, gypsogenic, and sumaresinolic acids) isolated from Miconia species, along with a mixture of ursolic and oleanolic acids and a mixture of maslinic and 2-a-hydroxyursolic acids, as well as ursolic acid derivatives were evaluated against the following microorganisms: Streptococcus mutans, Streptococcus mitis, Streptococcus sanguinis, Streptococcus salivarius, Streptococcus sobrinus, and Enterococcus faecalis, which are potentially responsible for the formation of dental caries in humans. The microdilution method was used for the determination of the minimum inhibitory concentration (MIC) during the evaluation of the antibacterial activity. All the isolated compounds, mixtures, and semi-synthetic derivatives displayed activity against all the tested bacteria, showing that they are promising antiplaque and anticaries agents. Ursolic and oleanolic acids displayed the most intense antibacterial effect, with MIC values ranging from 30 microg/mL to 80 microg/mL. The MIC values of ursolic acid derivatives, as well as those obtained for the mixture of ursolic and oleanolic acids showed that these compounds do not have higher antibacterial activity when compared with the activity observed with either ursolic acid or oleanolic acid alone. With regard to the structure-activity relationship of triterpene acids and derivatives, it is suggested that both hydroxy and carboxy groups present in the triterpenes are important for their antibacterial activity against oral pathogens.

Journal ArticleDOI
TL;DR: Three new triterpene glycosides, bacopasides VI-VIII (1-3), together with three known analogues, b Bacopaside I, I, and II showed antidepressant activity when tested on forced swimming and tail suspension in mice, respectively.
Abstract: Three new triterpene glycosides, bacopasides VI-VIII (1-3), together with three known analogues, bacopaside I (4), bacopaside II (5), and bacopasaponsin C (6), were isolated from the whole plant of Bacopa monnieri. Compounds 4, 5, and 6 showed antidepressant activity when tested on forced swimming and tail suspension in mice, respectively.

Journal ArticleDOI
TL;DR: The principal constituents, floratheasaponins A-F, were found to show the inhibitory activity on the release of beta-hexosaminidase from RBL-2H3 cells.
Abstract: The methanolic extract from the flower buds of Chinese tea plant (Camellia sinensis (L.) O. KUNTZE) was found to inhibit release of beta-hexosaminidase from RBL-2H3 cells. From the methanolic extract, six new acylated oleanane-type triterpene oligoglycosides, floratheasaponins D-I, were isolated together with 21 known compounds including floratheasaponins A-C. The chemical structures of floratheasaponins D-I were elucidated on the basis of chemical and physicochemical evidence. The principal constituents, floratheasaponins A-F, were found to show the inhibitory activity on the release of beta-hexosaminidase from RBL-2H3 cells.

Journal ArticleDOI
TL;DR: Five new dammarane-type triterpene glycosides, floralquinquenosides A, B, C, D, and E, were isolated from the flower buds of American ginseng and elucidated on the basis of chemical and physicochemical evidence.
Abstract: Five new dammarane-type triterpene glycosides, floralquinquenosides A, B, C, D, and E, were isolated from the flower buds of American ginseng, Panax quinquefolium L., together with 18 known dammarane-type triterpene glycosides and 3 flavonoid glycosides. The structures of new floralquinquenosides were elucidated on the basis of chemical and physicochemical evidence.

Journal ArticleDOI
TL;DR: Six new dammarane-type triterpene diglycosides with a hydroperoxide group, floralginsenosides A, B, C, D, E, and F, were isolated from ginseng flower and elucidated on the basis of chemical and physicochemical evidence.
Abstract: Six new dammarane-type triterpene diglycosides with a hydroperoxide group, floralginsenosides A, B, C, D, E, and F, were isolated from ginseng flower, the flower buds of Panax ginseng C. A. MEYER, together with seven known dammarane-type triterpene oligoglycosides. The structures of new floralginsenosides were elucidated on the basis of chemical and physicochemical evidence.

Journal ArticleDOI
TL;DR: Formation of seco-amyrins by this OSC revealed that OSCs not only catalyze carbon-carbon bond formations and Wagner-Meerwein rearrangements but also cleave preformed ring systems in cationic intermediates, and direct production of other naturalSeco-triterpenes by O SCs is proposed.
Abstract: At1g78500, one of the oxidosqualene cyclase (OSC) homologues from Arabidopsis thaliana, was expressed in a lanosterol synthase-deficient yeast strain and the products were analyzed. In addition to the known triterpenes, this OSC was found to produce two new triterpenes, the structures of which were determined by NMR and MS analyses. The new triterpenes are C-ring-seco-beta-amyrin (1) and C-ring-seco-alpha-amyrin (2) and named beta-seco-amyrin and alpha-seco-amyrin, respectively. beta-seco-Amyrin is produced from the oleanyl cation through bond cleavage between C8 and C14, and alpha-seco-amyrin is produced from the ursanyl cation in the same manner. Together with Grob fragmentation catalyzed by another OSC (marneral synthase) from A. thaliana, the formation of seco-amyrins by this OSC revealed that OSCs not only catalyze carbon-carbon bond formations and Wagner-Meerwein rearrangements but also cleave preformed ring systems in cationic intermediates. Based on this information, direct production of other natural seco-triterpenes by OSCs is proposed.

Journal ArticleDOI
TL;DR: It is demonstrated that HMGR2 as well as HMGR1 is responsible for the biosynthesis of triterpenes in spite of the lack of visible phenotypes in hmg2.
Abstract: Plants produce a wide variety of cyclic triterpenes, such as sterols and triterpenoids, which are the major products of the mevalonate (MVA) pathway. It is important to understand the physiological functions of HMG-CoA reductase (HMGR) because HMGR is the rate-limiting enzyme in the MVA pathway. We have previously isolated Arabidopsis mutants in HMG1 and HMG2. Although the biochemical function of HMGR2 has been thought to be almost equal to that of HMGR1, based on similarities in their sequences, the phenotypes of mutants in these genes are quite different. Whereas hmg2 shows no abnormal phenotype under normal growth conditions, hmg1 shows pleiotropic phenotypes, including dwarfing, early senescence, and male sterility. We previously postulated that the 50% decrease in the sterol content of hmg1, as compared to that in the wild type, was a cause of these phenotypes,1) but comprehensive triterpene profiles of these mutants had not yet been determined. Here, we present the triterpene profiles of hmg1 and hmg2. In contrast to hmg1, hmg2 showed a sterol content 15% lower than that of the wild type. A precise triterpenoid quantification using synthesized deuterated compounds of β-amyrin (1), α-amyrin (2), and lupeol (3) showed that the levels of triterpenoids in hmg1 and hmg2 were 65% and 25% lower than in the wild type (WT), respectively. These results demonstrate that HMGR2 as well as HMGR1 is responsible for the biosynthesis of triterpenes in spite of the lack of visible phenotypes in hmg2.

Journal ArticleDOI
TL;DR: The saponin showed in vitro antifungal activity against all the twenty fungal test isolates including ATCC strain and was found to be most effective against Trychophyton mentagrophytes and Sporothrix schenckii, MIC range of 1.56 microg/ml.

Journal ArticleDOI
TL;DR: Three new cytotoxic oleanane-type triterpenoid saponins, gummiferaosides A-C, showed cytotoxicity against the A2780 human ovarian cancer cell line with IC50 values of 0.8, 1.5, and 0.6 microg/mL, respectively.
Abstract: Bioassay-guided fractionation of an EtOH extract obtained from the roots of the Madagascan plant Albizia gummifera led to the isolation of three new cytotoxic oleanane-type triterpenoid saponins, gummiferaosides A−C (1−3). The structures of these new compounds were elucidated using 1D and 2D NMR experiments and mass spectrometry. Compounds 1−3 showed cytotoxicity against the A2780 human ovarian cancer cell line with IC50 values of 0.8, 1.5, and 0.6 μg/mL, respectively.

Journal ArticleDOI
TL;DR: Based on sequence comparisons and product profiles, the molecular evolution of triterpene synthases and the involvement of these genes in the formation of terpenoids in mangrove leaves are discussed.
Abstract: Oleanane-type triterpene is one of the most widespread triterpenes found in plants, together with the lupane type, and these two types often occur together in the same plant. Bruguiera gymnorrhiza (L.) Lamk. and Rhizophora stylosa Griff. (Rhizophoraceae) are known to produce both types of triterpenes. Four oxidosqualene cyclase cDNAs were cloned from the leaves of B. gymnorrhiza and R. stylosa by a homology-based PCR method. The ORFs of full-length clones termed BgbAS (2280 bp, coding for 759 amino acids), BgLUS (2286 bp, coding for 761 amino acids), RsM1 (2280 bp, coding for 759 amino acids) and RsM2 (2316 bp coding for 771 amino acids) were ligated into yeast expression plasmid pYES2 under the control of the GAL1 promoter. Expression of BgbAS and BgLUS in GIL77 resulted in the production of beta-amyrin and lupeol, suggesting that these genes encode beta-amyrin and lupeol synthase (LUS), respectively. Furthermore, RsM1 produced germanicol, beta-amyrin, and lupeol in the ratio of 63 : 33 : 4, whereas RsM2 produced taraxerol, beta-amyrin, and lupeol in the proportions 70 : 17 : 13. This result indicates that these are multifunctional triterpene synthases. Phylogenetic analysis and sequence comparisons revealed that BgbAS and RsM1 demonstrated high similarities (78-93%) to beta-amyrin synthases, and were located in the same branch as beta-amyrin synthase. BgLUS formed a new branch for lupeol synthase that was closely related to the beta-amyrin synthase cluster, whereas RsM2 was found in the first branch of the multifunctional triterpene synthase evolved from lupeol to beta-amyrin synthase. Based on these sequence comparisons and product profiles, we discuss the molecular evolution of triterpene synthases and the involvement of these genes in the formation of terpenoids in mangrove leaves.

Journal ArticleDOI
TL;DR: Two new triterpene glycosides, namely holothurin A3 and A4 were isolated from the methanol extract of the sea cucumber, Holothuria scabra, with their structures elucidated from the spectroscopic evidence.
Abstract: Two new triterpene glycosides, namely holothurin A3 (1) and A4 (2) were isolated from the methanol extract of the sea cucumber,Holothuria scabra, with their structures elucidated from the spectroscopic evidence (1D NMR, 2D NMR, ESI-MS and HRESI-MS). Compounds1 and2 were found to be strongly cytotoxic to both cancer cell lines, KB and Hep-G2, with 50% inhibitory concentrations (IC50) of 0.87 and 0.32 μg/mL (for compound 1) and of 1.12 and 0.57 μg/mL (for compound2), respectively.

Journal ArticleDOI
TL;DR: The seasons before flowering and at the beginning of that stage appeared to be the most efficient periods for leaf collection in reference to triterpene aglycone contents in plant yield.
Abstract: The qualitative and quantitative evaluation of triterpene aglycones of saponin fractions isolated from vegetative and generative organs of three Silphium species, Silphium perfoliatum, Silphium trifoliatum, and Silphium integrifolium, as compared to materials used in the herbal industry such as Panax quinquefolium root and Calendula officinalis flower, was performed. The analyses revealed that triterpene aglycones of saponins isolated from tested Silphium and Calendula species were oleanolic acid and ursolic acid. It was found that Panax roots contained only the aglycone of oleanolic acid within the triterpene saponin group. The leaves of Silphium harvested in May were characterized by the highest content of oleanolic acid-They contained 17.03 mg/g dry weight of the triterpenic acid, on average. The seasons before flowering and at the beginning of that stage appeared to be the most efficient periods for leaf collection in reference to triterpene aglycone contents in plant yield. Moreover, it was found that inflorescences of S. trifoliatum and S. integrifolium contained oleanolic acid in amounts of 22.05 and 17.95 mg/g dry weight respectively, whereas Calendula flowers contained 20.53 mg/g dry weight. The oleanolic acid content in Panax roots was 3.15 mg/g dry weight. Ursolic acid most abundantly occurred in S. integrifolium and S. trifoliatum at concentrations of about 14.98 mg/g dry weight in leaves harvested before flowering (June) and to 15.50 mg/g dry weight in leaves collected during flowering.

Journal ArticleDOI
TL;DR: Among the triterpenoid saponins isolated from a MeOH extract of the leaves of Ilex oblonga, three compounds showed obvious activities in inhibiting multiplication of the tobacco mosaic virus (TMV).
Abstract: Ten triterpene saponins have been isolated from a MeOH extract of the leaves of Ilex oblonga. In their structures, six new triterpenoid saponins were named as oblonganosides H-M (2, 4, 5, and 8-10). All structures were elucidated on the basis of spectroscopic analysis. Among the triterpenoid saponins, three compounds (7, 8, and 10) showed obvious activities in inhibiting multiplication of the tobacco mosaic virus (TMV).

Journal ArticleDOI
Xiaolong Chang1, Wei Li1, Zhonghua Jia1, Tadaaki Satou1, Shinji Fushiya1, Kazuo Koike1 
TL;DR: The cytotoxic activities of saponins 1-21 are reported against three human cancer cell lines, namely, HL-60 myeloid leukemia, KATO-III stomach adenocarcinoma, and A549 lung adenOCarcinomas cells.
Abstract: Eleven new triterpenoid saponins, ardisianosides A (1), B (2), C (4), D (5), E (6), F (7), G (15), H (16), I (17), J (18), and K (19), together with 10 known saponins, were isolated from the whole plants of Ardisia japonica. The structures of the new saponins were established on the basis of extensive 1D and 2D NMR and MS studies coupled with chemical degradations. The cytotoxic activities of saponins 1−21 are reported against three human cancer cell lines, namely, HL-60 myeloid leukemia, KATO-III stomach adenocarcinoma, and A549 lung adenocarcinoma cells.

Journal ArticleDOI
TL;DR: Ampelozizyphus amazonicus Ducke is a potential source of bioactive compounds since its extracts and fractions isolated from it exhibited in vitro parasite lysis against trypomastigote forms of T. cruzi.
Abstract: Ampelozizyphus amazonicus Ducke is a tree commonly found in the Amazon region and an extract of its stem bark is popularly used as an antimalarial and anti-inflammatory agent and as an antidote to snake venom. Ursolic acid; five lupane type triterpenes: betulin, betulinic acid, lupenone, 3beta-hydroxylup-20(29)-ene-27,28-dioic acid, and 2alpha,3beta-dihydroxylup-20(29)-ene-27,28-dioic acid, and three phytosteroids: stigmasterol, sitosterol and campesterol, have been isolated from stem extracts of A. amazonicus Ducke. Their structures were characterized by spectral data including COSY and HMQC. In an in vitro biological screening of the isolated compounds, 3beta-hydroxylup-20(29)-ene-27,28-dioic acid was cytotoxic against the SKBR-3 human adenocarcinoma cell line (1 to 10 mg/mL), while 2alpha,3beta-dihydroxylup-20(29)-ene-27,28-dioic acid exhibited cytotoxicity against both SKBR-3 human adenocarcinoma and C-8161 human melanoma tumor cell lines (>0.1 mg/mL). In the present study, different extracts and some fractions of this plant were also investigated for trypanocidal activity due to the presence of pentacyclic triterpenes. The triterpene classes are potent against Trypanosoma cruzi. The bioassays were carried out using blood collected from Swiss albino mice by cardiac puncture during the parasitemic peak (7th day) after infection with the Y strain of T. cruzi. The results obtained showed that A. amazonicus is a potential source of bioactive compounds since its extracts and fractions isolated from it exhibited in vitro parasite lysis against trypomastigote forms of T. cruzi at concentrations >100 microg/mL. Fractions containing mainly betulin, lupenone, 3beta-hydroxylup-20(29)-ene-27,28-dioic acid, and 2alpha,3beta-dihydroxylup-20(29)-ene-27,28-dioic acid showed more activity than crude extracts.

Journal ArticleDOI
TL;DR: Two new compounds with tigliane and cycloartane skeletons, isolated from the roots of Euphorbia guyoniana, are established on the basis of physical and spectroscopic analysis and by comparison with the literature data.

Journal ArticleDOI
TL;DR: Three (1,2,4) and one known (3) triterpenoid saponins were isolated from the bark of Albizia procera and exhibited cytotoxicity against HEPG2 cell line with IC50 9.13 microg/ml and 10 microg /ml, respectively.