Showing papers on "Typing published in 1974"

Pyocin Typing of Pseudomonas aeruginosa

Abstract: Over the last 10 years, pyocin typing has been a useful tool for the epidemiological fingerprinting of Pseudomonas aeruginosa The widely used "scrape-andstreak" method of Gillies and Govan, although simple to perform, has not been sensitive enough in differentiation of strains Use of additional indicator strains might correct this deficiency The "growth-in-broth" method of typing has shown promise; because it is easily automated, it may become the method of choice when very sensitive results are required The value of pyocin typing appears to be well established in the study of nosocomial infections; however, in some situations it may give misleading results Further studies are required for demonstration of the relative value and relationships of serological, pyocin, and bacteriophage typing

Combined biochemical and serological typing of clinical isolates of Klebsiella.

Abstract: In a series of 640 strains of Klebsiella isolated from clinical specimens over a 7-month period, there were sufficient biochemical differences between strains to allow a biochemical typing system to be established. Biochemical tests were done in solid media inoculated with a modified Steers inocula replicator. Biotypes were designated by a numerical coding system; 29 distinct biotypes were found among the 640 strains of Klebsiella. Serotyping of 270 of the strains was done by the Quellung reaction, and 40 capsular types were identified. Numerical biotypes and serotypes of strains appeared to vary independently. When used in conjunction, the two methods subdivided the strains into many more distinct types than either used alone. With the combined method over 100 types of Klebsiella were distinguished among the 270 isolates.

A simple double immunodiffusion test for typing influenza viruses

Abstract: The identification of influenza virus type has traditionally been based on the characterization of internal nucleoprotein (NP) antigens by the complement fixation (CF) test Because this test is complex and time-consuming, it is used only infrequently In this report we describe a double immunodiffusion (DID) test, which is proposed as a replacement for the CF test for the typing of influenza viruses The DID test is simple and requires only the allantoic fluid content of a single infected embryonated egg Virus from the infected fluid is precipitated with mild acid and disrupted by detergent Anti-NP or anti-matrix protein (MP) antibody is equally effective in most instances for typing influenza isolates by this test The latter is preferred, however, since it seemed to be slightly more sensitive than anti-NP antibody

Clinical and Epidemiological Correlates of Pseudomonas Typing

Abstract: Strains of Pseudomonas aeruginosa isolated from patients at Walter Reed Army Medical Center over a three-year period have been studied by three laboratory assays: serotyping, antibiotic sensitivity, and pyocin typing. Serotyping with use of rabbit antisera to the seven immunotype strains of Fisher has proved to be a simple, rapid method for differentiation of strains. When this was combined with a quantitative test of sensitivity to gentamicin, it was possible to analyze a number of clinical and epidemiological problems. Although pyocin typing was more cumbersome and gave somewhat variable results, it yielded correlative information in certain cases.

Resistotyping of Proteus mirabilis and a comparison with other methods of typing

Abstract: Resistotyping of P. mirabilis using 10 compounds is reported. The method was tested for reproducibility and specificity and results were compared with those obtained by serological, bacteriophage, and proticine typing methods and the Dienes test. The possible relationship between resistance to the chemicals used in the test and antibiotics was also studied. The method was found to be simple, reproducible, show good specificity, and compare favourably with other typing methods. No linkage between resistance to the chemicals and antibiotic resistance was detected.

Aeruginocine typing of Pseudomonas aeruginosa

Abstract: Aeruginocine typing using eight indicator strains of Wahba on Bacto tryptone-glucose yeast extract Agar (Difco) with an incubation temperature of 32°C was found to be a reproducible and easy method which could be readily adopted by a diagnostic clinical laboratory. The results of aeruginocine typing of 1500 strains of Ps. aeruginosa suggest that hospitals differ in the range and patterns of aeruginocine types of strains isolated in them. In addition to representatives of nine of the 10 pyocine types of Wahba, a large number of strains isolated in India were found to fall into other groups not described in Wahba's system. Hence, 20 new inhibition patterns (aeruginocine types) are proposed.

Determination of Genome Size of Selected Typing Bacteriophages of Staphylococcus aureus

Abstract: The molecular weights of six representative typing bacteriophages were determined by sucrose gradient centrifugation. Genome size appeared to be related to the size of the phage head.

Mumps meningoencephalitis in Stockholm. V. Virus isolations from samples of cerebrospinal fluid and urine--a comparison between some cell systems and typing techniques.

Abstract: Four different cell systems are compared with regard to usefulness for the isolation of mumps virus and several techniques, in particular hemadsorption inhibition (HDI) and immunofluorescence (IF), are evaluated for efficiency and rapidity in typing of mumps virus isolates. It appears that with suitable cells, such as a line of green monkey kidney cells (GMK, AH-1), and with typing by IF (or alternatively, by HDI) virus isolation is a fairly rapid and efficient tool for the laboratory diagnosis of mumps.

[Serological typing of Staphylococcus epidermidis --comparison between human and bovine strains].

Abstract: ヒト臨床材料およびウシ乳汁中より表支ブドウ球菌をそれぞれ135株, 341株分離し, serum-softagar法を応用して血清学的に型別し, 比較検討を行つた。これら菌株をserum-soft agar法で発育型を調べたところ, ヒト由来株では22.2%, 乳汁由来株では56.6%がcompact型を示し, ヒト由来株に比較して乳汁由来株では高頻度にcompact型が検出されたoヒト臨床材料よりえた血清型の異なる10種の表皮ブドウ球菌をウサギに免疫してえた抗血清を用いてserum-softagar法で血清型別を行つたところ, ヒト由来株では94.3%, ウシ乳汁由来株では83.1%を型別しえた。型別しえたヒト由来株99株中38株が単独型で10型に分類され, 61株は多価型のパターンを示し, 37型に分類された。これらのうち33型, ないし408型の因子血清と反応する菌株が約75.5%認められたが, M-10, 404, 411, N-4型などの因子血清と反応する菌株は数株認められるにすぎなかつた。一方, 乳汁由来株では型別された123株中45株が単独型で8型に分類され, 78株は多価型のパターンを示し, 48型に分類された。単独型ではM-10型と反応する株が全菌株の12.8%を占め, ヒト臨床材料から多数検出された53単独型は認められず, 多価型においても53あるいは21型と反応する菌株の数は少なかつた。また, ヒト由来株ではほとんど認められなかつた404, 411, N-4型などの血清型は多岐に亘る因子血清と反応する菌株が多く, 中でも39型因子血清と反応する株が多く認められた。なお, 408型, 53型はヒト臨床材料, 乳汁など広い分野から多数検出されたことは, こめ両者の株が広範囲に分布しているものと想像された。