Topic
Typing
About: Typing is a research topic. Over the lifetime, 5010 publications have been published within this topic receiving 146539 citations.
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TL;DR: Examination of additional enzyme loci should increase the sensitivity of the method for typing and for overall assessment of genetic relationships between spirochaetes, which was not always as discriminatory as DNA-restriction endonuclease analysis.
51 citations
TL;DR: This chapter explains the practical procedures currently available for the characterization of V. cholera using various epidemiological typing procedures, as well as a working background of the relevant theoretical knowledge.
Abstract: Publisher Summary Cholera is a disease of bacterial origin. It is not only important to recognize the disease clinically, but also to attempt as complete an identification of the causative organism as possible using various epidemiological typing procedures. This chapter explains the practical procedures currently available for the characterization, as well as a working background of the relevant theoretical knowledge. V. cholera is asporogenous, single curved or rigid rod with a single polar flagellum. It is indophenols oxidase positive and produces acid without gas from glucose. The aim of all typing systems is to identify strains within a species with a degree of precision that makes it safe to assume that all the isolates from an epidemic are truly identical and have therefore originated from a single parent strain. Of the three methods of typing V. cholera—serotyping, phage-typing and bacteriocin biotyping—serological typing was the earliest to be developed.
50 citations
TL;DR: A MICA typing method based on polymerase chain reaction (PCR)/sequence-based typing and a computer program that determines the polymorphisms and distinguishes the GCT repeats in exon 5 is developed, which is presently the method of choice for high-resolution clinical MICA typed.
50 citations
TL;DR: Genotyping for the major human platelet antigen (HPA) systems HPA‐1 through HPA-5 is of considerable help in establishing the diagnoses of these diseases.
50 citations
TL;DR: The value of phage typing for tracking the transmission of tuberculosis in the community is explored as well as documenting congenital transmission of infection and distinguishing exogenous reinfection from endogenous reactivation.
Abstract: Mycobacteriophage typing of Mycobacterium tuberculosis isolates was used as an epidemiologic aid in investigating the transmission of tuberculosis in community, industrial, and institutional outbreaks. The technique was also useful in other situations, e.g., documenting congenital transmission of infection and distinguishing exogenous reinfection from endogenous reactivation. Additional studies are indicated to further explore the value of phage typing for tracking the transmission of tuberculosis in the community.
50 citations