Topic
Typing
About: Typing is a research topic. Over the lifetime, 5010 publications have been published within this topic receiving 146539 citations.
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TL;DR: The patterns of hybridization were highly polymorphic but one endonuclease, BamHI, defined sequences unique to all HLA-Dw/DR specificities 1-8 except HLA/DR 2 and 6; however, these two specificities were resolved with the enzyme EcoRI.
Abstract: Sequences of different sizes are generated when DNA from homozygous HLA-Dw/DR typing cells are digested with restriction endonuclease and analyzed by hybridization with a HLA-D region class II antigen beta-chain cDNA probe. The patterns of hybridization were highly polymorphic but one endonuclease, BamHI, defined sequences unique to all HLA-Dw/DR specificities 1-8 except HLA-Dw/DR 2 and 6; however, these two specificities were resolved with the enzyme EcoRI. Digestion with other endonucleases such as Pst I results in patterns of restriction fragments that differ between homozygous typing cells of the same HLA-Dw/DR specificity. HLA-D region beta-chain probes permit HLA-D region genotyping at the DNA level and may allow detection of genes controlling the association of HLA specificities with a wide variety of diseases.
50 citations
TL;DR: A typing system for group-B streptococci, based on the use of 24 phages, used in conjunction with serotyping, gave highly discriminating and reproducible results.
Abstract: A typing system for group-B streptococci, based on the use of 24 phages, has been evaluated. Used in conjunction with serotyping, it gave highly discriminating and reproducible results.
50 citations
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49 citations
TL;DR: MALDI-ToF MS has potential as a rapid and reproducible platform for the identification and typing of Mycobacterium species and was developed and validated and matched completely to classical spoligotyping data.
49 citations
TL;DR: The molecular typing system for Treponema pallidum using cerebrospinal fluid specimens obtained from patients with neurosyphilis in Pretoria, South Africa shows that the typing system can be applied to specimens which may contain low numbers of spirochaetes such as CSF.
Abstract: Objective: To evaluate the molecular typing
system for Treponema pallidum using cerebrospinal fluid
specimens obtained from neurosyphilis patients in
Pretoria, South Africa. Methods: CSF specimens were collected from 32 men
and 18 women with suspected late neurosyphilis. Typing
of T. pallidum involved PCR amplification and
restriction analysis of the tpr E, G, and J genes and
determination of the number of 60-bp tandem repeats
within the arp gene by PCR amplification.
Results: Of 13 typeable specimens, 4 strain types
were identified: 2i, 3e, 14a, and 17e. Subtype 14a was
identified in 7 specimens (53.8%) whilst 4 specimens
(30.7%) were subtype 3e, and 1 specimen (7.6%) each had
subtype 17e and 2i. Conclusions: This study shows that the typing
system can be applied to specimens which may contain low
numbers of spirochetes such as cerebrospinal fluid.
49 citations