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Topic

Typing

About: Typing is a research topic. Over the lifetime, 5010 publications have been published within this topic receiving 146539 citations.


Papers
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Journal ArticleDOI
TL;DR: PCR-RFLP typing is a sensitive and specific method to identify and characterize rapidly HPV DNA in clinical specimens from a variety of sources and can differentiate between most known types of HPV and discriminate between infections with single, multiple or novel HPV types.

161 citations

Journal Article
TL;DR: A passive hemagglutination technique employing isolated γ-globulins and myeloma proteins of known genetic types coated onto human group O cells by CrCl3 method has been developed and cells thus coated have been successfully used for Gm and Inv typing of human sera.
Abstract: A passive hemagglutination technique employing isolated γ-globulins and myeloma proteins of known genetic types coated onto human group O cells by CrCl3 method has been developed. Cells thus coated have been successfully used for Gm and Inv typing of human sera. The degree of discrimination between inhibiting and noninhibiting sera is as great as with the conventional method using cells coated with incomplete anti-Rh.

161 citations

Journal ArticleDOI
TL;DR: In this paper, the authors used serial swab specimens from the oropharynges and anuses and tracheal and gastric aspirates from patients in an intensive care unit during a 10-month period in a setting of endemicity.
Abstract: Colonization with Pseudomonas aeruginosa was studied by taking serial swab specimens from the oropharynges and anuses and tracheal and gastric aspirates from patients in an intensive care unit during a 10-month period in a setting of endemicity. Nineteen (10%) of the 192 patients included in the study were colonized on admission, while another 30 (16%) patients acquired P. aeruginosa while in the hospital. Typing of 353 isolates was performed by random amplified polymorphic DNA (RAPD) analysis, and 56 strains were selected for further typing by RAPD analysis, pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) analysis. By these methods, 42, 44, and 44 genotypes were found, respectively. Computer-aided cluster analysis indicated that similar groups of related isolates were obtained by each method. By taking admission periods into account, analysis of the typing results suggested cross-acquisition of P. aeruginosa for five patient pairs. The small number of transfers and the large number of genotypes found indicate that most P. aeruginosa strains were derived from the patients themselves. The numbers of observed typing patterns and band differences between related isolates were counted for each typing method. AFLP analysis with primers without a selective base proved to be the most discriminatory method, followed by PFGE, AFLP analysis (with one selective base), and RAPD analysis. On the basis of a comparison with established strain differentiation criteria for PFGE, the criteria for differentiation of P. aeruginosa by AFLP analysis are presented.

161 citations

Journal ArticleDOI
Jeffrey M. Farber1
TL;DR: This review focuses on the basics of molecular typing along with the advantages and disadvantages of several of the newer genotypic typing techniques, such as plasmid typing, pulsed-field gel electrophoresis, ribotyping and its variations, and polymerase chain reaction-based methods such as random amplified polymorphic DNA analysis.

160 citations

Journal ArticleDOI
TL;DR: A method is described for typing Staphylococcus aureus capsular polysaccharides that is based on direct bacterial cell agglutination and immunoprecipitation of cell extracts with monospecific antisera.
Abstract: A method is described for typing Staphylococcus aureus capsular polysaccharides that is based on direct bacterial cell agglutination and immunoprecipitation of cell extracts with monospecific antisera. Encapsulated strains were identified by their inagglutinability with teichoic acid antisera. The typing sera reacted specifically with extracts of eight prototype strains.

154 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023329
2022690
2021145
2020126
2019136
2018147