Showing papers on "Upstream activating sequence published in 1980"
TL;DR: Sequences which are essential for the initiation of specific transcription in vitro were shown to be located between 12 and 32 base pairs upstream from the 5' end of these genes.
Abstract: In vitro genetic techniques were used to study the sequence requirements for the initiation of specific transcription. Deletion mutants were constructed around the putative promoter of the adenovirus-2 major late and chicken conalbumin genes. Specific transcription in vitro by RNA polymerase B together with a HeLa cell cytoplasmic extract was used as the test for promoter function. With this approach sequences which are essential for the initiation of specific transcription in vitro, were shown to be located between 12 and 32 base pairs upstream from the 59 end of these genes.
817 citations
TL;DR: Combined DNA and RNA sequence analyses strongly suggest that the major polysomal RNA may be generated from a transcription unit with a promoter at position 39, even though this transcription unit is part of a larger transcriptionunit with an upstream promoter near position 6.
157 citations
TL;DR: Characterization of recombinant lac promoters highlights the importance of two regions of sequence conservation in promoters for RNA polymerase binding in vitro and levels of expression in vivo.
Abstract: Characterization of recombinant lac promoters highlights the importance of two regions of sequence conservation in promoters. The "Pribnow box" sequences are necessary for specific transcription in this system. This specificity is maintained when a mutated upstream sequence is introduced. However, changing the upstream DNA sequences influences both the rate of RNA polymerase binding in vitro and levels of expression in vivo.
27 citations