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Upstream activating sequence
About: Upstream activating sequence is a research topic. Over the lifetime, 1633 publications have been published within this topic receiving 100112 citations.
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TL;DR: Both in vitro as well as in vivo experiments, using a cell line which endogenously expresses the 5-HT(7) receptor, indicated that mithramycin A, an inhibitor of Sp1/3 transcription factor binding, was able to inhibit 5- HT( 7) promoter activity.
7 citations
TL;DR: The major promoter is defined and several positive and negative upstream regulatory domains are identified employing deletion analysis and transient expression in HepG2 cells and it is demonstrated the formation of multiple specific DNA/protein complexes with protein factor(s) present in hepatitisG2 nuclear extract.
7 citations
TL;DR: Genetic evidence is provided that the upstream-activating sequences (UAS), which serve as the binding sites for the pWW0-encoded XylR protein (the m-xylene-responsive σ54-dependent activator of Pu), isolate expression of the upper TOL genes from any adventitious transcriptional flow originating further upstream.
7 citations
TL;DR: The control of mRNA synthesis in the unicellular eukaryote Saccharomyces cerevisiae involves a number of promoter elements, including an upstream activation site (UAS), an RNA initiation element (RIE) and, for some genes, a form of negative element.
Abstract: The control of mRNA synthesis in the unicellular eukaryote Saccharomyces cerevisiae involves a number of promoter elements, including an upstream activation site (UAS), an RNA initiation element (RIE) and, for some genes, a form of negative element. The UAS is involved in the activation and regulation of transcription, whilst the RIE, which comprises a transcription initiation site (or I site), and often a TATA box, is responsible for the accurate positioning of the 5′ end of the mRNA. The mechanism whereby these promoter elements interact involves specific protein-DNA binding events and possibly alterations in chromatin structure.
7 citations
TL;DR: Deletion analysis of the OPI1 promoter region, disruption of ICRE in it, and its activity in ino2- and ino4-disrupted strains showed that ICRE is essential for the expression of O PI1, and that the expression is dependent on the INO2 and INO4 gene products.
7 citations