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Upstream activating sequence

About: Upstream activating sequence is a research topic. Over the lifetime, 1633 publications have been published within this topic receiving 100112 citations.


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Journal ArticleDOI
TL;DR: This analysis has shown that the conformation of functionally relevant sites changes as a function of sequence mutations that have taken place elsewhere; this shows that the conformational behavior of the whole promoter region is linked and suggests transmission of topological effects in RNA polymerase II promoters.

7 citations

Patent
05 Jun 1995
TL;DR: In this article, a recombinant nucleotide sequence, characterized by a regulatory sequence of the initiation of transcription, was introduced. But the sequence coding for a polypeptide, which is different from that naturally associated with the promoter, was not considered.
Abstract: The invention relates to a recombinant nucleotide sequence, characterized in that it comprises: a regulatory sequence of the initiation of transcription, this regulatory sequence containing a promoter in association with the motif GCACTC 9N GAGTGC, in which "N" signifies any one of the 4 bases thymine, guanine, adenine and cytosine; a sequence coding for a polypeptide, called "heterologous polypeptide", which is different from that naturally associated with the promoter; the coding sequence being positioned downstream from the regulatory sequence of the initiation of transcription at a site which, under suitable conditions, would allow the expression of the polypeptide under the control of the promoter.

7 citations

Patent
03 Mar 2003
TL;DR: In this article, a vector comprising a first promoter sequence which constitutively or conditionally regulates transcription, a first transcription structure including a DNA-binding region, a nucleus localization signal sequence, an AhR-ligand binding control region and a transcriptional activation region, which is arranged downstream of the first promoters sequence, one or more second promoters sequence which is specifically coupled with the DNA-interaction region, and a second transcription structure, including a reporter gene, was provided a transformant with the vector and a method for monitoring and/or reducing the AhRligand.
Abstract: There is provided a vector comprising a first promoter sequence which constitutively or conditionally regulates transcription, a first transcription structure including a DNA-binding region, a nucleus localization signal sequence, an AhR-ligand binding control region and a transcriptional activation region, which is arranged downstream of the first promoter sequence, one or more second promoter sequence which is specifically coupled with the DNA-binding region, thereby to transcribe a transcription unit under control of the transcriptional activation region, and a second transcription structure including a reporter gene, which is arranged downstream of the second promoter sequence. Also, there are provided a transformant with the vector, and a method for monitoring and/or reducing the AhR-ligand.

6 citations

Journal ArticleDOI
TL;DR: The upstream element of the pIX gene appears to have a novel function: suppression of the transcriptional repression exerted by a downstream sequence, leading to a net transcription activation.
Abstract: cis-Acting elements involved in transcription of the peptide IX (pIX) gene of adenovirus 2 were identified by using in vivo transient expression assays and two in vitro transcription systems. Deletion of either the sequence between positions -45 and -70 or the TATA box abolished the initiation of pIX gene transcription in vivo and transcription with HeLa cell nuclear extracts in vitro. These results initially suggested the presence of a positive factor acting on the upstream element. However, when proteins in the nuclear extract were fractionated by column chromatography and analyzed by reconstitution of transcription in vitro, it was found that a certain fraction could direct TATA box-dependent transcription initiation even in the absence of the upstream element. Furthermore, activity inhibiting TATA box-dependent transcription was found in the nuclear extract. In contrast, inhibition of TATA box-dependent transcription was suppressed by deletion of a downstream sequence between positions +33 and +122. These results indicate that the TATA box of the pIX gene by itself has the ability to direct initiation of constitutive transcription but that the function of this element is under negative control by a repressor acting on a downstream sequence. Thus, the upstream element of the pIX gene appears to have a novel function: suppression of the transcriptional repression exerted by a downstream sequence, leading to a net transcription activation. Possible mechanisms for transcription initiation of pIX DNA are discussed.

6 citations

Journal ArticleDOI
TL;DR: A 0.9-kb fragment situated directly upstream of the first ATG of rabbit FKBP52 is rich in acceptor sites for transcription factors, contains a CAAT box at -197 and could represent the proximal promoter of this immunophilin, suggesting that these transcription factors could be involved in the regulation of the gene in both species.
Abstract: A 0.9-kb fragment situated directly upstream of the first ATG of rabbit FKBP52 is rich in acceptor sites for transcription factors, contains a CAAT box at -197 and could represent the proximal promoter of this immunophilin. Transvection analysis of this fragment showed strong promoter activity on the expression of a reporter gene. Deletions at the 5' end of this fragment showed that a basic sequence of 155 base pairs upstream of the CAAT box was sufficient to enhance luciferase expression an average 220-fold compared to the empty vector. This sequence, which contains acceptor sites for transcription factors of the EGR family and heat-shock factors, is closely homologous to 110 base pairs situated directly 5' of FKBP52 exon 1 in human chromosome 12p13.3, suggesting that these transcription factors could be involved in the regulation of the gene in both species. Furthermore, the upstream region of RbFKBP52 contains a large proportion of SINEs (C-repeats, Alu analogs), some of which include the A and B boxes required for transcription of RNA polymerase III, and poly A tracts. RbFKBP52, like HuFKBP52, is made up of 10 exons and 9 introns, a feature shared with other large immunophilins such as FKBP65 and Cyclophilin 40, and which appears widely conserved.

6 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20232
20223
20218
20206
20196
20186