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Upstream activating sequence

About: Upstream activating sequence is a research topic. Over the lifetime, 1633 publications have been published within this topic receiving 100112 citations.


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Journal ArticleDOI
TL;DR: Review on ATF4 (activating transcription factor 4 (tax-responsive enhancer element B67)), with data on DNA, on the protein encoded, and where the gene is implicated.
Abstract: Review on ATF4 (activating transcription factor 4 (tax-responsive enhancer element B67)), with data on DNA, on the protein encoded, and where the gene is implicated.

2 citations

Journal Article
TL;DR: Deletion analysis on the fused PHO81-lacZ gene revealed two important regions in the upstream sequences ofPHO81 gene -401 -289 bp and -1 012 -801 bp that may contain upstream activation sequence (UAS) and upstream enhancer sequence.
Abstract: Deletion analysis on the fused PHO81-lacZ gene revealed two important regions in the upstream sequences of PHO81 gene -401 -289 bp and -1 012 -801 bp. They did not share higher similarity with the upstream regions of PHO5 and PHO84 gene, except that -401 -289 bp contains the 5'-CACGTG/T-3' motif, which was found among the upstream regions of PHO5 and PHO84 gene and was the core sequence of PHO4 binding site it also contains A/T-rich segments flanking the motif, which may be PHO2 binding sites. This suggests that the -401 -289 bp of the upstream region of PHO81 gene may contain upstream activation sequence (UAS), and -1 012 -801 bp may contain upstream enhancer sequence. The gel retardation assays of the -1 012 -801 bp was performed using yeast total protein extract, and the results showed that there was an unknown protein factor binding at the region.

2 citations

Patent
28 Dec 1990
TL;DR: An artificial promoter for expressing proteins in yeast, comprising a sub-sequence upstream from the TATA element of the Saccharomyces cerevisiae gene GAL7 promoter sequence, which comprises the upstream activating sequences UAS1 and UAS2, was proposed in this paper.
Abstract: An artificial promoter for expressing proteins in yeast, comprising a sub-sequence upstream from the TATA element of the Saccharomyces cerevisiae gene GAL7 promoter sequence, which comprises the upstream activating sequences UAS1 and UAS2; and a sub-sequence of an ADH2 promoter sequence comprising the TATA element and the transcription initiating region. Uses include obtaining proteins, oxidase urate in particular.

2 citations

01 Jan 2012
TL;DR: Analysis of activated transcription by Gal4-fusion activators and the beta-Actin gene (ACTB) promoter upstream activating sequences further demonstrates that preferential communication between activator and core promoters contributes to the gene expression regulation.
Abstract: OF THE DISSERTATIONMechanisms of Core Promoter Sequence-dependent RNA Polymerase II TranscriptionbyMuyu XuDoctor of Philosophy, Graduate Program in Biochemistry and Molecular BiologyUniversity of California, Riverside, December 2012Dr. Ernest Martinez, ChairpersonThe TATA-box, Initiator (INR), Downstream Promoter Element (DPE), Motif Ten Element (MTE), TFIIB Recognition Element (BRE) and the other core promoter elements contribute to the diverse architecture of core promoters and are paramount for transcriptional activation. Diverse core promoters can communicate with enhancer-bound activators to contribute in the second level gene expression regulation. However, the mechanisms of transcription initiation catalyzed by different core promoters remain unknown and/or controversial. Because TFIID and TFIIB bind most of the core promoter elements, many scientists believe that different core promoters are regulated by the same set of general transcription factors. In contrast, other scientists including us insist that additional core promoter sequence-specific transcription factors besides the general transcription machinery are required to regulate transcription from different core promoters. Several lines of evidence support this hypothesis: a TAFs and Initiator dependent Cofactor 1 (TIC1) fraction requires for the TATA/INR synergy; a TIC2 fraction supports TATA-less core promoter directed transcription; CK2, PC4 and Mediator facilitate the Sp1-activated INR/DPE transcription in mammalian system and NC2 mediates the INR/DPE transcription in Drosophila system. Here, we further purify the TIC1 fraction and identified HMGA1 and Mediator as the effective components that support TATA/INR synergy in vitro. In addition, we also verify the TATA/INR specific role of HMGA1 in mammalian cells. Furthermore, we demonstrate HMGA1 interacts with TFIID and Mediator, and the acidic COOH-tail of HMGA1 is required but not sufficient for HMGA1 to interact with TFIID and Mediator. Accordingly, HMGA1 COOH-tail is also required to support the maximal transcriptional synergy between the TATA-box and the INR. Finally, analysis of activated transcription by Gal4-fusion activators and the beta-Actin gene (ACTB) promoter upstream activating sequences further demonstrates that preferential communication between activators and core promoters contributes to the gene expression regulation. Amazingly, a strict TATA-specificificity by the ACTB upstream activating sequences is revealed for the first time.

2 citations

Journal ArticleDOI
TL;DR: Interestingly, the CYP1-UAS′ complex is importantly diminished and the transcription of CYP3 is insensitive to the wild-type CYP2-activating protein, suggesting that a spatial organization of the promoter might lead to the reconstitution in vivo of an active UAS1-like sequence.
Abstract: Cyp1p (Hap1p) activates, among others, the two structural genes, CYC1 and CYP3 (CYC7) which encode isocytochromes c in Saccharomyces cerevisiae. This activation is believed to occur through the binding of the protein to the dissimilar upstream activation sequences (UASs), UAS1 and UAS′, present upstream of CYC1 and CYP3, respectively. In this paper, we describe a novel promoter mutation, CYP3-5, which results from a 39-bp deletion located about 160 bp upstream of the well-characterized CYP3 UAS. This deletion includes a sequence identical to the 3′ moiety of the CYC1 UAS1. Strikingly, a sequence identical to the 5′ part of the CYC1 UAS1 is also present 60 bp downstream of the 3′ half in the wild-type gene, suggesting that a spatial organization of the promoter might lead to the reconstitution in vivo of an active UAS1-like sequence. Interestingly, we find that in the presence of the CYP3-5 mutation, which disrupts this potential UAS1, the CYP1-UAS′ complex is importantly diminished and the transcription of CYP3 is insensitive to the wild-type CYP1-activating protein.

2 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20232
20223
20218
20206
20196
20186