Topic
Upstream activating sequence
About: Upstream activating sequence is a research topic. Over the lifetime, 1633 publications have been published within this topic receiving 100112 citations.
Papers published on a yearly basis
Papers
More filters
01 Jan 2007
1 citations
•
TL;DR: The work presented here suggests that TG is specific toward UAS containing plasmid and has the potential for use as nonviral DNA delivery agent.
1 citations
••
TL;DR: The chapter describes a range of reporter gene systems and the way they can be applied in the study of yeast gene expression and focuses on the principles involved and provides illustrative examples.
Abstract: Publisher Summary This chapter focuses on reporter genes and their use in studying yeast gene expression. Reporter genes have been established as valuable tools for the investigation of individual steps of eukaryotic gene expression. Typically, the sequences to be analyzed for regulatory function are combined with the reading frame of a reporter gene whose product can be readily assayed quantitatively. The effects of the sequence under study on specific steps of the yeast expression pathway are monitored via the reporter gene activity. The reporter gene systems can have a wide range of applications. In terms of monitoring gene function, these include the identification and characterization of sequences that influence transcription, pre-mRNA processing, and mRNA translation and decay. The chapter describes a range of reporter gene systems and the way they can be applied in the study of yeast gene expression. It focuses on the principles involved and provides illustrative examples. The coverage of the chapter is restricted to reporter genes that encode products subject to convenient and quantitative methods of detection. “The yeast” referred to in the chapter is Sacchromyces cerevisiae, although some of the methods and strategies discussed can be used with other yeast species also.
1 citations
••
TL;DR: In this paper, a two-step transcriptional activation (TSTA) was used to detect dynamic changes in intron sequences, with their loss and gain, due to the limited methods for the non-invasive monitoring of the pre-mRNA splicing process.
1 citations
••
TL;DR: In this article, the authors used an upstream activating sequence (UAS)-GFP transgenic reporter to determine whether Nur77 is transcriptionally active in hematopoietic cells in vitro.
1 citations