Topic
Uronic acid
About: Uronic acid is a research topic. Over the lifetime, 2091 publications have been published within this topic receiving 114935 citations.
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TL;DR: In this article, a method was developed to determine submicro amounts of sugars and related substances using a phenol-sulfuric acid reaction, which is useful for the determination of the composition of polysaccharides and their methyl derivatives.
Abstract: Simple sugars, oligosaccharides, polysaccharides, and their derivatives, including the methyl ethers with free or potentially free reducing groups, give an orangeyellow color w-hen treated with phenol and concentrated sulfuric acid. The reaction is sensitive and the color is stable. By use of this phenol-sulfuric acid reaction, a method has been developed to determine submicro amounts of sugars and related substances. In conjunction with paper partition chromatography the method is useful for the determination of the composition of polysaccharides and their methyl derivatives.
45,381 citations
TL;DR: A new method for determination of uronic acids with meta-hydroxydiphenyl is introduced, which is simpler, quicker, more sensitive, and more specific than other methods, and it needs lesser amounts of fluid.
6,302 citations
TL;DR: It has been found possible to distinguish betweenHeparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures by modifying Dische's carbazole reaction for uronic acid in the presence of borate.
5,798 citations
TL;DR: Xanthan gum, the extracellular polysaccharide from Xanthomonas campestris, has been reinvestigated by methylation analysis, and by uronic acid degradation followed by oxidation and elimination of the oxidized residue.
724 citations
TL;DR: In this article, a colorimetric assay utilizing m-hydroxydiphenyl is recommended because of its low background readings in the presence of large quantities of neutral sugars.
Abstract: Reproducible determinations of cell wall uronic acid content are obtained by first dissolving wall samples in sulfuric acid and then performing assays on aliquots of the cell wall solution The colorimetric assay utilizing m-hydroxydiphenyl is recommended because of its low background readings in the presence of large quantities of neutral sugars
658 citations