Topic
Vanadate
About: Vanadate is a research topic. Over the lifetime, 4497 publications have been published within this topic receiving 120109 citations. The topic is also known as: vanadate.
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TL;DR: In this paper, a modified Czochralski method was used to grow Nd3+-doped gadolinium vanadate (Nd:GdVO4) single crystals by maintaining a high temperature gradient at the interface during growth.
Abstract: We have successfully grown Nd3+-doped gadolinium vanadate ( Nd:GdVO4) single crystals by a modified Czochralski method in which a high temperature gradient was maintained at the interface during growth. Step faceting occurred during crystal growth and the cause of the faceting is discussed in relation to the growth conditions. Laser diode (LD) pumped laser oscillations were demonstrated. The Nd:GdVO4 single crystal showed superior lasing properties to Nd:YVO4 and Nd:YAG single crystals.
57 citations
TL;DR: In this paper, a precipitation method was used on the reaction between cerium nitrate hexahydrate and ammonium metavanadate in water, and three capping agents such as glucose, lactose and starch were used to investigate their effects on the morphology and particle size of CeVO4 nanoparticles.
Abstract: In this paper, cerium vanadate (CeVO4) nanoparticles were synthesized via a precipitation method on the reaction between cerium nitrate hexahydrate and ammonium metavanadate in water. Besides, three capping agents such as glucose, lactose and starch were used to investigate their effects on the morphology and particle size of CeVO4 nanoparticles. According to the vibrating sample magnetometer, cerium vanadate (CeVO4) nanoparticles indicated a paramagnetic behavior at room temperature. To evaluate the catalytic properties of nanocrystalline cerium vanadate, the photocatalytic degradations of methyl orange under ultraviolet light irradiation were carried out. The structural, morphological, magnetic, and optical properties of as-obtained products were characterized by techniques such as XRD, SEM, EDX, VSM, and UV–Vis spectroscopy.
57 citations
TL;DR: The present report on binding site determinations on mammalian kidney microsomes before and after detergent treatment casts serious doubts on the general validity of the above simple relationship between sites and peptide composition.
Abstract: THREE types of high affinity ligands are available for studies with (Na+ + K+)ATPase: nucleotide substrates, vanadate and ouabain. Of these, the last is unique in apparently being absolutely specific for (Na+ + K+)ATPase. One of the generally accepted facts regarding (Na+ + K+)ATPase is that there are equal numbers of measurable (high affinity) binding sites for ATP and ouabain per molecule of enzyme1–5. There is a majority opinion that direct binding experiments yield binding capacities for these ligands of 1 mol site per 250–300 kg protein in the purest preparations available3,6–8. From these data, from determinations of the peptide composition and from estimates of the apparent molecular weight of solubilised enzyme the idea of (Na+ + K+)ATPase as a dimer (with respect to the large, so-called catalytic, peptide) has emerged9–14. Such a dimer would have one high affinity binding site for nucleotide and one for ouabain. The present report on binding site determinations on mammalian kidney microsomes before and after detergent treatment casts serious doubts on the general validity of the above simple relationship between sites and peptide composition.
57 citations
TL;DR: A vanadate-sensitive H+-translocating ATPase isolated from red beet plasma membrane has been solubilized in active form and successfully reconstituted into artificial proteoliposomes.
Abstract: A vanadate-sensitive H+-translocating ATPase isolated from red beet plasma membrane has been solubilized in active form and successfully reconstituted into artificial proteoliposomes. The H+-ATPase was solubilized in active form with deoxycholate, CHAPSO or octylglucoside in the presence of glycerol. Following detergent removal by gel filtration and reconstitution into proteoliposomes, ATP:Mg-dependent H+ transport could be measured as ionophore-reversible quenching of acridine orange fluorescence. Solubilization resulted in a three-to fourfold purification of the plasma membrane ATPase, with some additional enrichment of specific activity following reconstitution. H+ transport activity was inhibited half-maximally between 1 and 5 μM vanadate (Na3VO4) and nearly abolished by 100 μM vanadate. ATPase activity of native plasma membrane showed aKi for vanadate inhibition of 9.5 μM, and was inhibited up to 80% by 15 to 20 μM vanadate (Na3VO4). ATPase activity of the reconstituted vesicles showed aKi of 2.6 μM for vanadate inhibition. The strong inhibition by low concentrations of vanadate indicates a plasma membrane rather than a mitochondrial or tonoplast origin for the reconstituted enzyme.
57 citations
TL;DR: It is concluded that decavanadate is much more potent mitochondrial depolarization agent and a more potent inhibitor of mitochondrial oxygen consumption than monomeric vanadate, pointing out the importance to take into account the contribution of higher oligomeric species of vanadium for the biological effects ofVanadate solutions.
57 citations