Topic
Vanadate
About: Vanadate is a research topic. Over the lifetime, 4497 publications have been published within this topic receiving 120109 citations. The topic is also known as: vanadate.
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52 citations
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51 citations
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TL;DR: The pp60v-src-hsp90 complex is stabilized by sodium molybdate with the same concentration dependence as the glucocorticoid receptor-hSp90 complex, and as with the steroid receptor heterocomplexes, vanadate and tungstate also stabilize the pp60 v- src- hsp90 interaction.
51 citations
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TL;DR: Negative stain electron microscopy of bundled microtubules revealed that they are arranged in parallel networks with extensive close lateral association, similar to dynein-like ATPase.
Abstract: We have prepared dynein-like ATPase from the eggs of the sea urchin Strongylocentrotus purpuratus using differential centrifugation and column chromatography. This ATPase preparation is inhibited by vanadate and erythro-9-(3-[2-hydroxynonyl]) adenine (EHNA) at concentrations similar to those that inhibit reactivated flagellar beating and spindle elongation in lysed cell models. Using microtubule affinity and ATP-induced release, we can purify this ATPase activity to a composition on SDS PAGE of four peptides ranging in molecular weight from 180,000-300,000. When viewed in darkfield optics, this affinity-purified ATPase caused extensive parallel bundling of microtubule-associated protein-free microtubules. These bundles were dispersed by 1 mM ATP but not by ATP gamma S or AMP-5'-adenylimidodiphosphate. The reformation of microtubule bundles after dispersal by ATP required ATP hydrolysis; bundles did not reform in the presence of 10 microM vanadate. Negative stain electron microscopy of these bundled microtubules revealed that they are arranged in parallel networks with extensive close lateral association.
51 citations
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TL;DR: The results suggest that two primary targets exist for vanadate in intact cells: a plasmalemma ATPase which energizes ion uptake and extrusion in Dunaliella and a flagellar ATPase responsible for motility.
51 citations