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Vanadate
About: Vanadate is a research topic. Over the lifetime, 4497 publications have been published within this topic receiving 120109 citations. The topic is also known as: vanadate.
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78 citations
78 citations
TL;DR: Data are consistent with the hypothesis that inhibition of the osteoblastic phosphotyrosyl protein phosphatases can prolong and/or potentiate the mitogenic actions of growth factors, and thereby stimulates cell proliferation.
Abstract: We recently proposed a hypothesis for the molecular mechanism of the osteogenic action of fluoride in which it stimulates osteoblast proliferation via the inhibition of an osteoblastic acid phosphatase-like phosphotyrosyl protein phosphatase activity. To test this hypothesis, we investigated whether orthovanadate, a known phosphotyrosyl protein phosphatase inhibitor, would mimic fluoride in the stimulation of bone cell proliferation and bone collagen synthesis in vitro. Orthovanadate inhibited the osteoblastic acid phosphatase activity and stimulated bone cell proliferation at the same low concentrations (i.e. 5-15 μM). At the mitogenic doses, orthovanadate also showed a dose-dependent increase in alkaline phosphatase (a marker of mature osteoblasts) in cultured calvarial cells and stimulated bone collagen synthesis, as measured by the incorporation of [3H]proline and the conversion into [3H] hydroxyproline in organ calvaria cultures. Therefore, orthovanadate stimulated bone formation by increasing the nu...
78 citations
TL;DR: It is demonstrated that H2O2 plays an important role in vanadium-induced NFAT transactivation in two different cell types, suggesting that H 2O2 may be involved in activation of calcium-calcineurin pathways for NFAT activation caused by vanadium exposure.
78 citations
01 Jan 1994
TL;DR: It is demonstrated that vanadium effects on protein tyrosine kinases are preserved after cell disintegration, supporting a working hypothesis implicating the intracellular vanadyl pool in modulating CytPTK activity.
Abstract: substrate concentrations. Preincubation of adipocytes with vanadyl(0.4 mM), and staurosporine (which arrests the cytosolic enzyme) sub- stantially inhibited insulin-stimulated lipogenesis. Va- nadyl is readily oxidized to vanadate by hydrogen per- oxide. In contrast, CytPTKs were poorly inhibited by vana- dyl, and vanadate stimulated several CytPTKs 2-6-fold. CytPTK derived from rat adipocytes, liver and brain were activated, and CytPTK from
78 citations