Showing papers on "Vascular endothelial growth factor A published in 1976"

Biosynthesis of collagen and metabolism of lipids by endothelial cells in culture.

Abstract: Endothelial cells are a ubiquitous type of epithelial cell that derive from mesenchyme and line all vascular channels. Epithelial tissue is characterized by the presence of a “free surface,” specialized intercellular junctions, and a basement membrane that serves to attach it to other connective tissue components. The “free surface” of endothelial cells comes into intimate contact with vascular components of the blood. Because under normal conditions such contact does not cause activation of the clotting mechanism, the surface of endothelial cells is considered to be non th rombogenic. Endothelial cells appear to play a role in the regulation of the transport of plasma constituents to the interstitium, including lipoproteins.22 Endothelial cells overlie a basement membrane that consists of a procollagenlike subunit associated with noncollagenous matrix g l y c o p r ~ t e i n s . ~ ~ The normal structure of basement membranes exhibits a variety of morphologic alterations in disease states, such as poststreptococcal glomerulonephritis, diabetes mellitus, Goodpasture’s syndrome, and scleroderma. Before attempts can be made to understand pathologic conditions, it is necessary to study the normal biochemical and metabolic properties of these cells. In 1973 and 1974, several laboratories reported on the successful cultureof endothelial cells derived from the human umbilical vein that were identified by various morphologic and immunochemical ~ r i t e r i a . ~ . I ~ * ~ ~ We have modified the above method and applied it for the cultureof calf aorta endothelial cells. This report describes studies on the biosynthesis of collagen and the metabolism of lipids by cultured calf and human endothelial cells.

Endothelial Proliferation Factor

Abstract: The viability of the brain is predicated upon its blood supply. Nowhere is the process of vascularization of the brain more dramatically visualized than in the expansion of the capillary bed within and around tumors of the CNS (6). Recent evidence suggests that this endothelial proliferation (or neovascularization as it is often called) is mediated by a soluble factor (12) released by the growing tumor cells as proposed earlier by Algire (1). The new vessels are recruited by the tumor from the already existent host vasculature in the region of the tumor (1, 26).