Showing papers on "Vascular endothelial growth factor A published in 1977"

Factors from 3T3 cells stimulate proliferation of cultured vascular endothelial cells.

Abstract: TUMOURS induce vascularisation into the neoplasm to provide needed cell nutrients and remove wastes1. The formation of tumour blood vessels seems to depend, in part, on the proliferation and movement of vascular endothelial cells which are, in turn, controlled by the secretion of tumour angiogenesis factor (TAP) (refs 2–4). Extracts of tumours2–4 and of established cell lines5 have TAF activity in vivo. But little is known about the molecular nature or mechanism of action of TAF. The proliferation, formation and integrity of vascular endothelium are also involved in normal host processes: wound healing, morphogenetic tissue rearrangements, as well as the prevention of various non-neoplastic diseases resulting from blood vessel wall injury depend on angiogenesis factors6. A convenient method to study angiogenesis and endothelial repair is with an in vitro endothelial cell system, but the lack of uniform, reliable cultured endothelial cell lines has delayed progress in this area. Most studies7–9 (with some exceptions10) have used uncloned vascular cells which are often slow growing and heterogeneous in cell type. We have recently established a cloned adult bovine aortic endothelial (ABAE) cell line which has maintained its endothelial properties for over 1 yr in culture, provided that fibroblast (growth factor (FGF) is present in the growth medium11. We report here the mitogenic effects of several untransformed and transformed cell lines on cloned ABAE cells and non-vascular endothelial cell controls.