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Vero cell

About: Vero cell is a(n) research topic. Over the lifetime, 3875 publication(s) have been published within this topic receiving 121312 citation(s). The topic is also known as: VERO & Verda reno.

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Open accessJournal ArticleDOI: 10.1038/NATURE02145
Wenhui Li1, Michael Moore1, Natalya Vasilieva2, Jianhua Sui3  +8 moreInstitutions (5)
27 Nov 2003-Nature
Abstract: Spike (S) proteins of coronaviruses, including the coronavirus that causes severe acute respiratory syndrome (SARS), associate with cellular receptors to mediate infection of their target cells Here we identify a metallopeptidase, angiotensin-converting enzyme 2 (ACE2), isolated from SARS coronavirus (SARS-CoV)-permissive Vero E6 cells, that efficiently binds the S1 domain of the SARS-CoV S protein We found that a soluble form of ACE2, but not of the related enzyme ACE1, blocked association of the S1 domain with Vero E6 cells 293T cells transfected with ACE2, but not those transfected with human immunodeficiency virus-1 receptors, formed multinucleated syncytia with cells expressing S protein Furthermore, SARS-CoV replicated efficiently on ACE2-transfected but not mock-transfected 293T cells Finally, anti-ACE2 but not anti-ACE1 antibody blocked viral replication on Vero E6 cells Together our data indicate that ACE2 is a functional receptor for SARS-CoV

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Topics: Coronavirus (63%), Vero cell (60%), Severe acute respiratory syndrome (53%) ...read more

4,123 Citations


Open accessJournal ArticleDOI: 10.1016/J.CELL.2020.04.004
14 May 2020-Cell
Abstract: We have previously provided the first genetic evidence that angiotensin converting enzyme 2 (ACE2) is the critical receptor for severe acute respiratory syndrome coronavirus (SARS-CoV), and ACE2 protects the lung from injury, providing a molecular explanation for the severe lung failure and death due to SARS-CoV infections. ACE2 has now also been identified as a key receptor for SARS-CoV-2 infections, and it has been proposed that inhibiting this interaction might be used in treating patients with COVID-19. However, it is not known whether human recombinant soluble ACE2 (hrsACE2) blocks growth of SARS-CoV-2. Here, we show that clinical grade hrsACE2 reduced SARS-CoV-2 recovery from Vero cells by a factor of 1,000-5,000. An equivalent mouse rsACE2 had no effect. We also show that SARS-CoV-2 can directly infect engineered human blood vessel organoids and human kidney organoids, which can be inhibited by hrsACE2. These data demonstrate that hrsACE2 can significantly block early stages of SARS-CoV-2 infections.

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Topics: Vero cell (51%)

1,234 Citations


Open accessJournal ArticleDOI: 10.1126/SCIENCE.1110656
10 Jun 2005-Science
Abstract: Ebola virus (EboV) causes rapidly fatal hemorrhagic fever in humans and there is currently no effective treatment. We found that the infection of African green monkey kidney (Vero) cells by vesicular stomatitis viruses bearing the EboV glycoprotein (GP) requires the activity of endosomal cysteine proteases. Using selective protease inhibitors and protease-deficient cell lines, we identified an essential role for cathepsin B (CatB) and an accessory role for cathepsin L (CatL) in EboV GP-dependent entry. Biochemical studies demonstrate that CatB and CatL mediate entry by carrying out proteolysis of the EboV GP subunit GP1 and support a multistep mechanism that explains the relative contributions of these enzymes to infection. CatB and CatB/CatL inhibitors diminish the multiplication of infectious EboV-Zaire in cultured cells and may merit investigation as anti-EboV drugs.

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Topics: Ebola virus (56%), Filoviridae (53%), Cathepsin B (53%) ...read more

761 Citations


Open accessJournal Article
01 Apr 1988-Cancer Research
Abstract: Normal human bronchial epithelial cells were infected with SV40 virus or an adenovirus 12-SV40 hybrid virus, or transfected via strontium phosphate coprecipitation with plasmids containing the SV40 early region genes. Colonies of morphologically altered cells were isolated and cultured; these cells had extended culture lifespans compared to normal human bronchial epithelial cells. All cultures eventually underwent senescence, with the exception of one which appears to have unlimited proliferative potential. Colonies arising after viral infection were screened for virus production by cocultivation with Vero cells; only viral nonproducer cultures were analyzed further. The cells retained electron microscopic features of epithelial cells, and keratin and SV40 T-antigen were detected by indirect immunofluorescence. All of the cultures were aneuploid with karyotypic abnormalities characteristic of SV40-transformed cells. No tumors formed after s.c. injection of the cells in nude mice. These cells should be useful for studies of multistage bronchial epithelial carcinogenesis.

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Topics: Transfection (55%), Vero cell (55%), Virus (52%) ...read more

752 Citations


Open accessJournal ArticleDOI: 10.1128/JVI.56.2.558-570.1985
Abstract: Using Vero cells transformed with the wild-type gene for ICP4 as the permissive host cell, we isolated herpes simplex virus type 1 (HSV-1) mutants containing deletions in both copies of the ICP4 gene The mutants, d120 and d202, contained deletions of 41 and 05 kilobases, respectively, in each copy of ICP4 ICP4 mRNA synthesized in d202-infected Vero cells was 05 kilobases smaller than that synthesized in cells infected with the wild-type virus No ICP4 mRNA was detected in d120-infected Vero cells d120 and d202 specified polypeptides that reacted with ICP4 antiserum and were smaller than the wild-type ICP4 polypeptide by 130 and 30 kilodaltons, respectively The only other HSV-1 gene products detectable on infection of Vero cells with d120 and d202 were ICP6 (of the early kinetic class of HSV-1 polypeptides), ICP0 (immediate early), ICP22 (immediate early), and ICP27 (immediate early) Immediate-early polypeptides ICP0 and ICP27 were expressed to a higher level in Vero cells infected with an ICP4 temperature-sensitive (ts) mutant (tsB32) at 39 degrees C, indicating immediate-early stimulatory activity associated with the ts ICP4 polypeptide In addition, the patterns of complementation of d120, d202, and tsB32 in ICP4-transformed cells also demonstrated inhibitory activity associated with the ts form of the ICP4 polypeptide

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Topics: Vero cell (63%), Herpes simplex virus (56%), Mutant (52%) ...read more

699 Citations


Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20222
2021181
2020156
2019115
2018103
201796

Top Attributes

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Topic's top 5 most impactful authors

Bernard Roizman

14 papers, 1.5K citations

Elsa B. Damonte

13 papers, 709 citations

Yukihiro Nishiyama

10 papers, 255 citations

Eladio Viñuela

9 papers, 348 citations

Ichiro Kurane

8 papers, 385 citations

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