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Withania somnifera

About: Withania somnifera is a research topic. Over the lifetime, 2116 publications have been published within this topic receiving 43404 citations. The topic is also known as: Ashwaganda & Indian ginseng.


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TL;DR: No significant change was observed in the activity of cardiac enzymes in baseline groups, and Protective actions of W. somnifera on heart have also been confirmed by gross pathology.
Abstract: The present study was designed to evaluate the preventive and curative cardioprotective potential of native and gemmotherapeutically treated on the basis of biochemical, histopathological and antioxidant parameters in the salbutamol (albuterol) induced myocardial necrosis in rabbits. Gemmotherapy is a newly emerging way of treatment and no work so far has been done on evaluation of curative cardioprotective potential of Withania somnifera. Rabbits were divided into eight main groups: Normal, Ischemia, WS Gemmo-baseline, WS Native-baseline, WS Gemmopreventive, WS Native-preventive, WS Gemmo-curative and WS Native-curative groups. Gemmo and native-baseline groups were treated with gemmo and native W. somnifera (50 mg/kg) for 20 days. Native and Gemmo-preventive groups were pre-treated with native and gemmotherapeutically treated W. somnifera at a dose 50 mg/kg for 3 weeks. On 20 th and 21 st day animals of all groups except normal and baseline groups were given Salbutamol (50mg/ kg), orally at an interval of 24 h. The Native-curative and Gemmo-curative groups were then treated with native and gemmotherapeutically treated W. somnifera at a dose 50 mg/ kg for 5 days, subsequent to the treatment with Salbutamol. Rabbits were subsequently sacrificed for gross pathological studies and hearts were removed for antioxidant assay. Administeration of Salbutamol significantly increased (p<0.05) the serum level of CK-MB, LDH, SGOT and SGPT in ischemia group as compared to Normal. However, pre- and post-treatment with Native and Gemmo extracts of W. somnifera significantly restored and reduced (p<0.05) the elevated serum levels of these cardiac markers. Also Native and Gemmo extracts of W. somnifera significantly increased (p<0.05) antioxidant enzymes; superoxide dismutase, catalase and glutathione peroxidase. No significant change was observed in the activity of cardiac enzymes in baseline groups. Protective actions of W. somnifera on heart have also been confirmed by gross pathology.

16 citations

01 Jan 2012
TL;DR: In this paper, a HPLC method using DAD and ELSD detector was developed for simultaneous determination of withaferine A and withanolide A from the roots and herbal formulation of the plant.
Abstract: Roots of Withania somnifera are widely used in Indian herbal formulations. Withaferine A and withanolide A are two pharmacologically important withanolides present in the roots of the plant. HPLC method using DAD and ELSD detector was developed for simultaneous determination of withaferine A and withanolide A from the roots and herbal formulation of the plant. Coarse powder of root was extracted with methanol by shaking for 30 minutes and sonication for 45 minutes. Herbal formulation was first extracted with chloroform and then with ethyl acetate, extracts were combined, evaporated and reconstituted with methanol. Retention time of both the compounds was vary with detector to detector and it was found for withaferine A (3.74, 3.84 min) and withanolide A (4.51, 4.60 min) with DAD and ELSD detector, respectively. Amount of withanolides in each sample were calculated from the regression equations of standard calibration graphs. The results showed that the HPLC method is suitable for the determination of withaferine A and withanolide A from the roots and herbal formulation of W. somnifera.

16 citations

Journal ArticleDOI
TL;DR: Ashwagandha ( Withania somnifera L.) Dunal] is an important medicinal plant and a major source of alkaloids and steroids (withanolids), which is regularly used in pharmaceutical industries and various vegetative parts were studied for its mass propagation through tissue culture technique.
Abstract: Ashwagandha ( Withania somnifera L.) Dunal] is an important medicinal plant and a major source of alkaloids and steroids (withanolids), which is regularly used in pharmaceutical industries. Various vegetative parts were studied for its mass propagation through tissue culture technique. Seeds were pretreated with GA3 (50 and 100 mgl -1 ) for 24 h and 80% germination was achieved. All the explants were taken from in-vitro germinated plant. Among the different explants tested, multiple shoot formation was achieved from shoot-tip and nodal explants in MS medium + 0.25, 0.5, and 1.0 mgl -1 kinetin. Nodal explants were selected for mass propagation protocol because it formed maximum number of shoots (16.25 shoots per explant) on MS medium + 1mgl -1 kinetin after eight weeks of culture. Increase in concentration of kinetin was most effective for callus formation. For further multiplication these shoots were sub-cultured on MS +0.5 mgl -1 kinetin. Presence of IAA at 0.5 mgl -1 was most effective medium for rooting of in-vitro propagated shoots. However, hardening was not achieved for these propagated plants. Key words: IAA; IBA; NAA; kinetin; in-vitro multiplication DOI: 10.3126/njst.v11i0.4131 Nepal Journal of Science and Technology 11 (2010) 101-106

16 citations

Journal ArticleDOI
TL;DR: Three fungal endophytes are reported from Withania somnifera, which could enhance withanolides content in leaf and root and induce a host resistant related gene, NPR1, resulting in 2, 4 and 16 fold expression levels in 2aWF, 10aWF and 5aWF endophyte treatments respectively, compared to control plants.
Abstract: Endophytes have been reported from all plant species from different parts of tissue including root, stem and leaves. Here we report, three fungal endophytes, Aspergillus terreus strain 2aWF (2aWF), Penicillium oxalicum strain 5aWF (5aWF), and Sarocladium kiliense strain 10aWF (10aWF) from Withania somnifera, which could enhance withanolides content in leaf and root. Upon treatment with the above endophytes to 4 weeks old plants in field conditions, W. somnifera elicited withanolide A content (97 to 100%) in leaves without considerable changes in withaferin A content. Furthermore, withanolide A content in roots of 5aWF and 10aWF endophyte treated W. somnifera plants increased up to 52% and 65% respectively. Incidentally, expression profile of withanolide and sterol biosynthetic pathway genes HMGR, DXR, FPPS, SQS, SQE, CAS, SMT1, STE1 and CYP710A1 were significantly upregulated in 2aWF, 5aWF and 10aWF fungal endophyte treated plants. Besides, modulation of withanolide biosynthetic pathway genes, fungal endophytes also induce a host resistant related gene, NPR1 resulting in 2, 4 and 16 fold expression levels in 2aWF, 10aWF and 5aWF endophyte treatments respectively, compared to control plants. Overall, our results illustrate that application of native-fungal endophytes 2aWF (96.60%), 5aWF (95%) and 10aWF (147%) enhances plant biomass in addition to withanolide content.

16 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023114
2022265
202188
2020124
201995
2018111