Withania somnifera

About: Withania somnifera is a research topic. Over the lifetime, 2116 publications have been published within this topic receiving 43404 citations. The topic is also known as: Ashwaganda & Indian ginseng.

Accumulation of Three Important Bioactive Compounds in Different Plant Parts of Withania somnifera and its Determination by the LC–ESI-MS-MS (MRM) Method

Abstract: A comprehensive experiment was conducted to study the accumulation pattern and determination of three important bioactive compounds namely withaferin-A (WA), 12-deoxywithastramonolide (WO) and withanolide-A (WD) and its determination by the liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS-MS) method in root, stem, fruits and leaves of Withania somnifera. A rapid and sensitive LC-ESI-MS-MS method was developed and validated for the determination of these three important bioactive compounds, having same molecular weight. The multiple reaction monitoring method was established by two transitions for each analyte and intense transition used for quantification. Separation of the three analytes was achieved within a run time of 5 min on an RP-18 column using a mobile phase consisting of acetonitrile and 0.1% acetic acid in water in an isocratic condition. The developed method was validated as per the ICH guidelines. The developed method was found to be suitable for identification and quantification of WA, WO and WD in different plant parts such as roots, stems, fruits and leaves of W. somnifera. The accumulation of WA was highest in leaves samples (8.84 ± 0.37 mg/g) and it was 2.23, 5.85 and 27.26 times higher than its concentration in fruits, stems and roots, respectively. WO and WD contents were highest (0.44 ± 0.016 and 0.72 ± 0.016 mg/g, respectively) in root.

STUDIES ON THE IMMUNOMODULATORY & THERAPEUTIC EFFICACY OF ASHWAGANDHA (Withania somnifera), GARLIC (Allium sativum) & TURMERIC (Curcuma longa) ON ENDOMETRITIC REPEAT BREEDING CROSSBRED COWS

Abstract: STUDIES ON THE IMMUNOMODULATORY & THERAPEUTIC EFFICACY OF ASHWAGANDHA (Withania somnifera), GARLIC (Allium sativum) & TURMERIC (Curcuma longa) ON ENDOMETRITIC REPEAT BREEDING CROSSBRED COWS

Characterization of an Insecticidal Protein from Withania somnifera Against Lepidopteran and Hemipteran Pest

Abstract: Lectins are carbohydrate-binding proteins with wide array of functions including plant defense against pathogens and insect pests. In the present study, a putative mannose-binding lectin (WsMBP1) of 1124 bp was isolated from leaves of Withania somnifera. The gene was expressed in E. coli, and the recombinant WsMBP1 with a predicted molecular weight of 31 kDa was tested for its insecticidal properties against Hyblaea puera (Lepidoptera: Hyblaeidae) and Probergrothius sanguinolens (Hemiptera: Pyrrhocoridae). Delay in growth and metamorphosis, decreased larval body mass and increased mortality was recorded in recombinant WsMBP1-fed larvae. Histological studies on the midgut of lectin-treated insects showed disrupted and diffused secretory cells surrounding the gut lumen in larvae of H. puera and P. sanguinolens, implicating its role in disruption of the digestive process and nutrient assimilation in the studied insect pests. The present study indicates that WsMBP1 can act as a potential gene resource in future transformation programs for incorporating insect pest tolerance in susceptible plant genotypes.

Antioxidant activity of Withania somnifera (L.) Dunal by different solvent extraction methods

Abstract: Withania somnifera (Solanaceae) Dunal commonly known as Ashwagandha, is an Indian medicinal plant that grows as an evergreen shrub in dry parts of India. Withania somnifera (L.) leaves, stem and root were screened for antioxidant activities by different solvent extraction methods. The extracts exhibited a good scavenging effect on DPPH, chelating activity and reducing power and the scavenging effect of the extracts was comparable with the standard ascorbic acid. The total phenolic content was determined as gallic acid equivalents. Among the different solvent extracts of each parts, methanol extract showed maximum antioxidant capacity than the other extracts like methanol > chloroform > acetone > ethyl acetate. . Various concentrations (0.10, 0.25, 0.50, 0.75 and 1.0 mg/ml) of the extracts (0.5 ml) was mixed with 2.5 ml of phosphate buffer (0.2 M, pH 6.6) and 2.5 ml of potassium ferricyanide (1%). The mixture was incubated at 500C for 20 min. TCA (10%: 2.5 ml) was added. The mixture was centrifuged at 650g for 10 min. The supernatant (2.5 ml) was mixed with 2.5 ml of distilled water and 0.5 ml of ferric chloride and the absorbance was measured at 700 nm in a Hitachi U-1500 spectrophotometer. Higher absorbance of the reaction mixture indicated greater reducing power. The reducing power of methanol, acetone, ethyl acetate and chloroform extracts was compared with that of L-ascorbic acid (0.2 mg/ml). RESULTS AND DISCUSSION Total phenolic content In most of the antioxidant study the phenolic compounds was directly correlated with its antioxidant ability. Therefore, in the present study it was considered imperative to analyze the total phenolic content (Folin-Ciocalteu method) of different solvent (methanol, ac- etone, ethyl acetate, and chloroform) extracts of different parts of Withania somnifera (L.) methanol, acetone, ethyl acetate and chloroform is shown in Figure: 1. Among the different solvents extracts of each part, methanol extract showed maximum phenolic content than the other extracts. As per the analysis the total phenolic content of different

Protective effect of administration of Withania somifera against bromobenzene induced nephrotoxicity and mitochondrial oxidative stress in rats.

Abstract: Background: The present study was conducted to elucidate the protective role of Withania somnifera against bromobenzene induced nephrotoxicity and mitochondrial dysfunction in rats. Methods: In this study, Wistar albino rats of either sex were divided into six groups consisting of six animals each. The first one was control, those in group II received bromobenzene (10 mmol/kg, intragastric intubation) once, but group III and IV animals received W. somnifera (250 and 500 mg/kg, orally), respectively for 8 days followed by bromobenzene once on the 8th day and silymarin (100 mg/kg, orally) was given for 8 days to group V animals and then bromobenzene on the last day. Group VI animals received only W. somnifera (500 mg/kg) for 8 days. Results: The levels of renal lipid peroxidation, lysosomal enzymes and glycoprotein were increased significantly (p < 0.05) in the bromobenzene alone treated rats and antioxidant status and mitochondrial enzymes were found to be decreased, when compared to the control gr...