scispace - formally typeset
Search or ask a question
Topic

Withania somnifera

About: Withania somnifera is a research topic. Over the lifetime, 2116 publications have been published within this topic receiving 43404 citations. The topic is also known as: Ashwaganda & Indian ginseng.


Papers
More filters
Journal ArticleDOI
TL;DR: This is the first study which revealed the effect of the seasonal variation on W. somnifera under natural field conditions in overall productivity, pathway gene expression and in withanolide biosynthesis.

9 citations

Journal ArticleDOI
TL;DR: The various stability indicating studies carried out in the present investigation would be useful for formulation development and were suggestive of deciding the recommended storage conditions for NMITLI-118RT+.
Abstract: Background: Withania somnifera Dunal (Ashwagandha) is an Indian medicinal plant of great medicinal value; used in many clinically proven conditions. NMITLI-118RT+ is a candidate drug under a Council of Scientific and Industrial Research (CSIR) networking project. It is a chemotype of W. somnifera's root extract, which has been used for the present study. Objectives: The present investigation aims to develop and validate a simple isocratic reverse phase-high performance liquid chromatography (RP-HPLC) system for the detection and estimation of Withanolide A (marker compound) and its analytical application for stability indicating studies on NMITLI-118RT+. Material and Methods: A validated RP-HPLC method for Withanolide A was established on a Waters HPLC system and the same was used on NMITLI-118RT+ for quantification and fingerprinting purposes, and for establishing forced degradation, isothermal stress tests, and drug-excipient testing protocols as per International Conference on Harmonization (ICH) guidelines. Results: A validated method was established, which could detect the marker at a retention time of around 6.3 minutes, with a linearity range of 2-100 μg/mL, by varying the amounts of the said marker, which were estimated in four different batches of NMITLI-118RT+. Photostability as per ICH guidelines suggested a slight loss of the active constituent and maximum degradation was afforded with alkali followed by acid, and then peroxide, in the forced degradation studies. In the drug-excipient studies, the maximum amount of active constituent could be detected in the samples with ethyl cellulose and the least with hydroxy propyl cellulose. Conclusion: The method developed here was simple and rapid. The various stability indicating studies carried out in the present investigation would be useful for formulation development and were suggestive of deciding the recommended storage conditions for NMITLI-118RT+.

9 citations

Journal ArticleDOI
TL;DR: Results obtained from this study suggests that A. indica and W. somnifera (aqueous and ethanolic) extracts may be attractive for the ‘drug hunters’ as a potential agent for the management of infectious diseases against the human pathogens.
Abstract: We studied effect of A. indica and W. somnifera crude extracts against the multi-drug resistant strains (MDR) and synergistic activity of A. indica and W. somnifera crude extracts on MDR strains (Agar well diffusion method). Both aqueous as well as alcoholic extracts of the plant (root as well as leaves) were found to possess strong antibacterial activity against a range of bacteria. Combination exhibited excellent synergy. The aqueous extract and ethanolic extract of A. indicaand W. somnifera exhibited very good synergistic activity against the MDR strain. Extracts were examined for its free radical scavenging activity by DPPH assay. IC50 value was found that 68.75 μg/ml (ethanolic extract) and 74.83 μg/ml (aqueous extract) of A. indica and 94.28 μg/ml (ethanolic extract) and 88.79 μg/ml (aqueous extract) of W. somnifera, which is significant when compared to solution of Ascorbic acid 50.58 μg/ml. Reducing ability of the crude extracts of Azadirachta indica (aqueous and ethanolic extract) and W. somnifera (aqueous and ethanolic extract), which is significant when compared to the standard butylatedhydroxy toluene (BHT). Results obtained from this study suggests that A. indica and W. somnifera (aqueous and ethanolic) extracts may be attractive for the ‘drug hunters’ as a potential agent for the management of infectious diseases against the human pathogens.

9 citations

Journal ArticleDOI
01 Feb 2022-Cancers
TL;DR: The data supported that this novel combination of Wi-A and CAPE may be recruited for the treatment of metastatic and aggressive cancers and, hence, warrant further evaluation by recruiting a variety of experimental and clinical metastatic models.
Abstract: Simple Summary Cancer therapy suffers from its high cost and high rate of adverse effects and relapse of the disease. Hence, the new (preferably natural), economic and safer therapeutic as well preventive measures have been on demand and have been subject of priority research. We have, earlier, demonstrated anticancer activity in the extracts of Ashwagandha leaves and propolis. A combination of Wi-A (an active anticancer ingredient in Ashwagandha extract) and CAPE (an active anticancer ingredient in propolis) was earlier shown to offer higher and cancer cell-selective cytotoxicity. In the present study, we report an anti-metastasis activity in the low dose combination of Wi-A and CAPE along with its mechanism of action and propose its use in cancer metastasis treatment. Abstract Withaferin A (Wi-A) and Caffeic Acid Phenethyl Ester (CAPE) are the bioactive ingredients of Ashwagandha (Withania somnifera) and propolis, respectively. Both of these natural compounds have been shown to possess anticancer activity. In the present study, we recruited a low dose of each of these compounds and developed a combination that exhibited remarkably potent anti-migratory and anti-angiogenic activities. Extensive molecular analyses including a cDNA array and expression analyses of the specific gene targets demonstrated that such activities are mediated through their effect on cell adhesion/tight junction proteins (Claudins, E-cadherin), inhibition of canonical Wnt/β-catenin signaling pathways and the consequent downregulation of EMT-signaling proteins (Vimentin, MMPs, VEGF and VEGFR) that play a critical role in cancer metastasis. The data supported that this novel combination of Wi-A and CAPE (Wi-ACAPE, containing 0.5 µM of Wi-A and 10 µM of CAPE) may be recruited for the treatment of metastatic and aggressive cancers and, hence, warrant further evaluation by recruiting a variety of experimental and clinical metastatic models.

9 citations

Journal Article
TL;DR: The effects of Cu+2 stress on growth and biochemical parameters of Withania somnifera L.Dunal, grown in a field pot experiment were studied as discussed by the authors, which indicated that Cu+ 2 stress negatively impacted nearly all the parameters assayed, particularly in the roots.
Abstract: The effects of Cu+2 stress (0, 10, 20, 50, 100 and 200 mM as CuSO4.5H2O) on growth and biochemical parameters of Withania somnifera L.Dunal, grown in a field pot experiment were studied. Increasing Cu+2 concentrations led to decreased stem length, root elongation and leaf area. Copper (Cu+2 ) stress decreased chlorophyll and carotenoids content in leaves and stems as compared to controls; this was more pronounced in leaves than in stems. Lipid peroxidation, in terms of malondialdehyde (MDA) content increased. Lipid peroxidation increased significantly with elevation in Cu+2 concentrations and was highest in roots followed by leaves and stems at 200 mM. Leakage of electrolytes measured in terms of membrane stability index (MSI), which showed a negative correlation with increasing Cu+2 levels. The MSI was lowest in 200 mM Cu+2 treated plants. The phenol content of leaves, stems and roots peaked at 50 mM Cu+2 treatment and thereafter declined. These results indicate that Cu+ 2 stress negatively impacted nearly all the parameters assayed; particularly in the roots. It is being reported for the first time that stress resistance in W.somnifera as observed by increase in total phenol content at low Cu+2 levels possibly to combat increased ROS generation.

9 citations


Network Information
Related Topics (5)
Antioxidant
37.9K papers, 1.7M citations
79% related
Essential oil
32.6K papers, 625.2K citations
78% related
Gallic acid
9.6K papers, 287K citations
78% related
Quercetin
7.7K papers, 333.3K citations
78% related
Antibacterial activity
18.4K papers, 322.4K citations
78% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023114
2022265
202188
2020124
201995
2018111