Topic
Withania somnifera
About: Withania somnifera is a research topic. Over the lifetime, 2116 publications have been published within this topic receiving 43404 citations. The topic is also known as: Ashwaganda & Indian ginseng.
Papers published on a yearly basis
Papers
More filters
TL;DR: In this article, eight new steroidal lactones of the withanolide series have been isolated from the leaves of Withania somnifera (Solanaceae) growing in North-Western India.
Abstract: Eight new steroidal lactones of the withanolide series have been isolated from the leaves of Withania somnifera(Solanaceae) growing in North-Western India. This population of W. somnifera differs from those investigated so far and constitutes a new chemotype. The new compounds (IV)—(XI) have been investigated by chemical degradation and interconversion reactions, as well as by analysis of their spectral properties. The following structures have been assigned: 5α,17α-dihydroxy-1-oxo-6α,7α-epoxy-22R-witha-2,24-dienolide (IV); 5α,17α-dihydroxy-1-oxo-22R-witha-2,6,24-trienolide (V); 5α,27-dihydroxy-1-oxo-6α,7α-epoxy-22R-witha-2,24-dienolide (VIa); 4β,17α-dihydroxy-1-oxo-5β,6β-epoxy-22R-witha-2,24-dienolide (VIIa); 17α,27-dihydroxy-1-oxo-22R-witha-2,5,24-trienolide (VIIIa); 7α,27-dihydroxy-1-oxo-22R-witha-2,5,24-trienolide (VIIIa); 7α,27-dihydroxyl-1-oxo-22R-witha-2,5,24-trienolide (IXa); 1α,3β,5α-trihydroxy-6α,7α-epoxy-22R-with-24-enolide (Xa); 4β-hydroxy-5β,6β-epoxy-1-oxo-22R-witha-2,14,24-trienolide (XIa).
65 citations
TL;DR: It is concluded that s-UV-B is a potent factor in increasing the concentrations of secondary metabolites and their biosynthetic pathway enzymes in W. somnifera.
Abstract: The present study aims to investigate the effects of supplemental ultraviolet B (3.6 kJ m(-2)day(-1) above ambient) radiation on secondary metabolites and phenylpropanoid pathway enzymes of Withania somnifera under field conditions at 40, 70, and 100 days after transplantation. Secondary metabolites' (alkaloids, anthocyanins, carotenoids, flavonoids, lignin, phytosterols, saponins, and tannins) concentrations were analysed at the end of the treatments. Activities of phenylalanine ammonia lyase (PAL), cinnamyl alcohol dehydrogenase (CAD), 4-coumarate-CoA ligase (4CL), chalcone-flavanone isomerase (CHI), and dihydroflavonol reductase (DFR) were also determined. In treated plants, secondary metabolite-concentrations generally increased (higher concentrations being recorded in roots compared to leaves). Anomalies were recorded for lycopene in roots and phytosterols in leaves (all sampling ages); β-carotene declined in leaves at third sampling age. s-UV-B-treated plants depicted decrease in withanolide A content with concomitant increase in withaferin A (two major alkaloids analysed by HPLC) compared to their respective controls. Phenylpropanoid pathway enzyme-activities increased in leaves and roots under s-UV-B treatment, the latter showing greater increase. The study concludes that s-UV-B is a potent factor in increasing the concentrations of secondary metabolites and their biosynthetic pathway enzymes in W. somnifera.
65 citations
TL;DR: The in vitro antimicrobial activity of the new withanolide (1) and the alcoholic fruit extract are discussed.
Abstract: Two new steroidal lactones of the withanolide-type, 5beta, 6alpha, 14alpha, 17beta, 20beta-pentahydroxy-1-oxo-20 S, 22R-witha-2, 24-dienolide (1)and 6alpha, 7alpha-epoxy-5alpha, 14alpha, 17alpha, 23beta-tetrahydroxy-1-oxo-22R-witha-2, 24-dienolide (2), were isolated from the fruit of Withania somnifera (L) Dunal (Solanaceae) growing in Southern Egypt, together with two known coumarins, scopoletin (3)and aesculetin (4), a known triterpene, viz Beta-amyrin (5), and two known phytosterols, viz stigmasterol (6) and sitosterol (7) The structures of the isolated compounds have been elucidated by spectroscopic methods including UV, IR, (1)H-, (13)C-NMR, DEPT experiments, HRFAB-, FAB-, and EI-mass spectrometry The in vitro antimicrobial activity of the new withanolide (1) and the alcoholic fruit extract are discussed
64 citations
TL;DR: Use of conductivity change in the medium for the on-line monitoring of cell mass growth was investigated and it was found that the effect of pH and sugar concentration changes on medium conductivity can be neglected.
64 citations
TL;DR: Two new withanolides named coagulin and withasomidienone have been isolated from Withania coagulance and Withania somnifera, respectively on the basis of spectroscopic studies.
Abstract: Two new withanolides named coagulin [17 beta,27-dihydroxy-14,20-epoxide-1-oxo-(22R)-witha-3,5,24-tr ienolide] and withasomidienone [27-hydroxy-3-oxo-(22R)-witha-1,4,24-trienolide] have been isolated from Withania coagulance and Withania somnifera, respectively. The structures have been determined on the basis of spectroscopic studies.
64 citations