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Xylanase

About: Xylanase is a research topic. Over the lifetime, 7099 publications have been published within this topic receiving 163793 citations.


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Journal ArticleDOI
TL;DR: A xylanase purified from Streptomyces rameus L2001 and the biobleaching effect on wheat straw pulp was investigated and was highly specific towards xylan, but did not exhibit other enzyme activity.

77 citations

Journal ArticleDOI
TL;DR: This study describes the xylanase production by Penicillium glabrum using brewer's spent grain as substrate, and is the first work that reports the purification and characterization of a xylan enzyme using this agroindustrial waste.
Abstract: In recent decades, xylanases have been used in many processing industries. This study describes the xylanase production by Penicillium glabrum using brewer's spent grain as substrate. Additionally, this is the first work that reports the purification and characterization of a xylanase using this agroindustrial waste. Optimal production was obtained when P. glabrum was grown in liquid medium in pH 5.5, at 25 °C, under stationary condition for six days. The xylanase from P. glabrum was purified to homogeneity by a rapid and inexpensive procedure, using ammonium sulfate fractionation and molecular exclusion chromatography. SDS-PAGE analysis revealed one band with estimated molecular mass of 18.36 kDa. The optimum activity was observed at 60 °C, in pH 3.0. The enzyme was very stable at 50 °C, and high pH stability was verified from pH 2.5 to 5.0. The ion Mn2+ and the reducing agents β-mercaptoethanol and DTT enhanced xylanase activity, while the ions Hg2+, Zn2+, and Cu2+ as well as the detergent SDS were strong inhibitors of the enzyme. The use of brewer's spent grain as substrate for xylanase production cannot only add value and decrease the amount of this waste but also reduce the xylanase production cost.

76 citations

Journal ArticleDOI
TL;DR: Xylanase was non-covalently immobilized on Eudragit S-100 for saccharification and produced xylanase with very low levels of cellulases when grown on 1% wheat bran medium.

76 citations

Journal ArticleDOI
TL;DR: Alkaliphilic and thermophilic Bacillus sp.
Abstract: Alkaliphilic and thermophilic Bacillus sp. strain TAR-1, isolated from soil, produced a xylanase extracellularly. The xylanase was most active over a pH range of 5.0 to 9.5 at 50°C. Optimum temperatures of the crude xylanase preparation were 75°C at pH 7.0 and 70°C at pH 9.0. Zymogram analyses of the culture supernatant showed that the molecular mass of the xylanase was 40 kDa and the isoelectric point was pH 4.1. The predominant products of xylan hydrolysate were xylobiose, xylotriose, and higher oligosaccharides, indicating that the enzyme was an endoxylanase. Production of the thermophilic alkaline xylanase was induced by xylan and xylose, but was repressed in the presence of glucose.

76 citations

Journal ArticleDOI
TL;DR: Mutant EU2-77 proved to be the most promising extracellular cellulase producer among 20 mutants in a screening program performed in shake flask fermentation after plate screening, rendering the supplementation of β-glucosidase unnecessary in waste newspaper hydrolysis.

76 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023199
2022463
2021254
2020289
2019278
2018303