Topic
Xylanase
About: Xylanase is a research topic. Over the lifetime, 7099 publications have been published within this topic receiving 163793 citations.
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TL;DR: RNAi to knock‐down (KD) multiple xylanase genes to elucidate their roles in the pathogenicity of the blast fungus, Magnaporthe oryzae indicated that xylanases play significant roles in both vertical penetration and horizontal expansion of M.’oryzae in infected plants.
Abstract: Due to functional redundancy, it is often difficult to genetically analyse the biological function of fungal cell wall-degrading enzymes that belong to a gene family. To overcome this difficulty, we used RNAi to knock-down (KD) multiple xylanase genes to elucidate their roles in the pathogenicity of the blast fungus, Magnaporthe oryzae. To obtain the maximum average efficiency of gene silencing for the xylanase genes, we used the 'building blocks method', in which a 40 bp sequence was chosen from an endoxylanase gene, and 10 such sequences from 10 endoxylanases were combined to make an artificial RNAi trigger by synthetic DNA. Quantitative RT-PCR analysis revealed that the transcript levels of all the expressed xylanase genes were significantly reduced in KD mutants with the artificial RNAi trigger. Even though the KD mutants did not completely lose their pathogenicity to host plants, the number of lesions, rate of penetration and extent of infected cells were all reduced in KD mutant-infected leaves. The degree of pathogenicity reduction was associated with the silencing levels of xylanase mRNA and enzymatic activity in the KD mutants. Cytological analysis indicated that xylanases play significant roles in both vertical penetration and horizontal expansion of M. oryzae in infected plants.
75 citations
TL;DR: This is the first ever report of IL-stable cellulase/xylanase enzyme from Aspergillus assiutensis, and mechanistic insights of combinatorial pretreatment physicochemical analysis of variously pretreated biomass were obtained.
75 citations
TL;DR: De-oiled Jatropha curcas seed cake, a plentiful by-product of biodiesel industry was used as substrate for the production of a useful xylanase from Sporotrichum thermophile in solid state fermentation, which seem potentially useful in the food industries.
75 citations
TL;DR: The study showed a promising approach can lead to an improvement in the lipid extraction yield from microalgae and further provide valuable information for the use of enzymes in microalgal processes.
75 citations
Journal Article•
TL;DR: In this article, microfibrillated cellulose (MFC) was isolated from bleached bagasse pulp pretreated with xylanase enzymes and returned to the pulp in varying amounts.
Abstract: To improve the properties of paper sheets, microfibrillated cellulose (MFC) was isolated from bleached bagasse pulp pretreated with xylanase enzymes and returned to the pulp in varying amounts. The ...
75 citations