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Xylanase
About: Xylanase is a research topic. Over the lifetime, 7099 publications have been published within this topic receiving 163793 citations.
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TL;DR: Purified XynS14 showed more endo-1,4-β-xylanase activity on xylan and xylooligosaccharides than on xylotriose, which may be due to protein-folding in the host.
71 citations
TL;DR: Sagohampas, the fibrous pith residue left after starch extraction from sago palm, is abundant at sago-processing factories and can be used as a substrate for the production of laccase by solid substrate fermentation (SSF) with Pleurotus sajorcaju, an edible mushroom.
Abstract: Sagohampas, the fibrous pith residue left after starch extraction from sago palm, is abundant at sago-processing factories and can be used as a substrate for the production of laccase by solid substrate fermentation (SSF) withPleurotus sajorcaju, an edible mushroom. The fungus grown onhampas with an adjusted carbon : nitrogen ratio of 35:1, exhibited high laccase activity together with variable cellulase (0.3-2.8 U/g) and xylanase (0.9-10.1 U/g) activity. The maximum amount of laccase produced was approximately 17.7 U/g after 6 days of SSF using 4-week-old inoculum at a density of 10%. With the mature four-week inoculum, laccase activity increased 12-fold compared to that achieved with two-week-old inoculum. The optimum pH and temperature of the crude laccase were 6.0 and 50‡C, respectively. The apparent Km and Vmax values obtained were 0.073 mM and 0.962 U/min, respectively. The maximum laccase activity could be almost doubled after 6 days of fermentation by addition of 0.2 mM vanillin or ferulic acid; the cellulose to lignin ratio increased significantly during the 12 days of SSF, from 2.74 in the control to 3.3, when 0.2 mM of either vanillin or ferulic acid was added to the substrate.
71 citations
TL;DR: The mature peptide of Bacillus licheniformis xylanase A was successfully expressed in Pichia pastoris under the control of AOX1 promoter and high performance liquid chromatography analysis revealed that xylotriose was the main hydrolysis product released from birchwood xylan and wheat bran insoluble xylan by reBlxA.
71 citations
TL;DR: A novel feruloyl esterase was discovered from a Fusarium proliferatum strain known to be capable of utilizing corn fiber xylan, and its unusual insensitivity to pH makes it a potentially useful enzyme in applications that require acidic or alkaline conditions.
71 citations
TL;DR: In this article, two 2 × 2 factorial experiments were conducted to investigate the interaction between xylanase (0 vs. 200 milligram/kg) and protease and showed an interaction between enzymes and growth performance.
71 citations