Topic
Xylanase
About: Xylanase is a research topic. Over the lifetime, 7099 publications have been published within this topic receiving 163793 citations.
Papers published on a yearly basis
Papers
More filters
••
TL;DR: Observation by fluorescence microscopy and analysis of the localization of beta-glucuronidase activity in transgenic A. thaliana plants containing a chimeric gene with the upstream sequence of the AtXyn1 gene and the GUS gene suggest that AtXn1 is involved in the secondary cell wall metabolism of vascular bundle cells.
Abstract: The cDNA clone RXF12, which encodes a xylanase (EC 3.2.1.8), was isolated from Arabidopsis thaliana. The C-terminal half of the amino acid sequence of the deduced protein, named AtXyn1, showed similarity with the catalytic domain of barley xylanase X-1. The N-terminal half of AtXyn1 also contained three regions with sequences similar to cellulose-binding domains (CBDs). A xylanase assay revealed that transgenic A. thaliana plants expressing exogenous AtXyn1 fused with enhanced green fluorescent protein (EGFP) possessed approximately twice as much xylanase activity as wild-type plants. Observation by fluorescence microscopy of transgenic A. thaliana plants expressing a fusion protein of AtXyn1 and EGFP suggested that AtXyn1 is a cell wall protein. Analysis of the localization of beta-glucuronidase (GUS) activity in transgenic A. thaliana plants containing a chimeric gene with the upstream sequence of the AtXyn1 gene and the GUS gene demonstrated that the AtXyn1 gene is predominantly expressed in vascular bundles, but not in vessel cells. These data suggest that AtXyn1 is involved in the secondary cell wall metabolism of vascular bundle cells. A database search revealed that four putative xylanase genes exist in the A. thaliana genome, besides the AtXyn1 gene. Of these, two also contain several regions with sequences similar to CBDs in their N-terminal regions. Comparison of the amino acid sequences of the five xylanases suggests a possible process for their molecular evolution.
61 citations
••
TL;DR: Phytotoxicity assay confirmed that metabolites generated after dye degradation by Y-BC-SH are non-toxic, and this multipurpose oleaginous yeast consortium was developed based on its higher ability to accumulate large amounts of lipids in the form of triacylglycerol.
61 citations
••
TL;DR: Sugarcane bagasse was used as substrate for xylanase production by means of a strain of Trichoderma harzianum Rifai isolated from decaying Aspidosperma sp.
61 citations
••
TL;DR: The high production of xylooligosaccharides (XOS) yield from the pretreated kenaf stem demonstrated the biotechnological potential of the kanaf inthe future.
60 citations
••
TL;DR: Xylooligosaccharides with DP 2-4 are important synbiotics used as food ingredients based on its prebiotic characteristics and the effect of sonication pretreatment on the conversion efficiency of the xylan preparation was investigated.
60 citations