Topic
Yeast
About: Yeast is a research topic. Over the lifetime, 31777 publications have been published within this topic receiving 868967 citations. The topic is also known as: yeasts.
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TL;DR: The fatty acid composition of the psychrophilic yeast remained fairly constant during its growth cycle, but that of the mesophilic yeast at 10° showed marked changes in the proportions of oleic and linoleic acids in the early stages of growth; similar changes occurred in the mesophile grown at 25°, but at much faster rates.
Abstract: The lipids of three psychrophilic yeasts of the genus Candida, grown at 10°, were compared with those of a mesophilic yeast (Candida lipolytica) grown at 25° and at 10°. All the yeasts examined had similar contents of total lipids and of phosphatides, and similar phosphatide and neutral lipid compositions. However, the total fatty acids of the psychrophiles and of the mesophile grown at 10° had much higher proportions of linoleic acid and lower proportions of oleic acid than those of the mesophilic yeast grown at 25°; the psychrophilic yeasts also contained linolenic acid, not detected in the mesophilic yeast. The fatty acid composition of the psychrophilic yeast remained fairly constant during its growth cycle, but that of the mesophilic yeast at 10° showed marked changes in the proportions of oleic and linoleic acids in the early stages of growth; similar changes occurred in the mesophile grown at 25°, but at much faster rates. On the basis of these observations and others it is concluded that in the ea...
189 citations
TL;DR: Improvements in yeast expression systems, coupled with the development of yeast surface display and refinements in two-hybrid methodology, are expanding the role of yeasts in the process of understanding and engineering eukaryotic proteins.
189 citations
TL;DR: It is proposed that the repetitive domain is a feature common to the nucleoporins, and cloned a gene encoding one of these proteins (NUP1) and the localization of the NUP1 protein to the pore complex by immunofluorescence is confirmed.
189 citations
TL;DR: Yeast incorporation improved survival 10%, and reduced malformed larvae, and in groups fed 1.1% yeast, only 1% of larvae were malformed, compared to 14% in the control group, suggesting earlier development of intestinal digestion.
189 citations
TL;DR: Gene cloning and yeast DNA transformation techniques have greatly enhanced the power of classical yeast genetics, making it possible to isolate any classically defined gene, to alter the yeast genome at will by replacing normal chromosomal sequences with mutated derivatives produced in vitro, and to create DNA molecules that behave as autonomous replicons or minichromosomes.
Abstract: Gene cloning and yeast DNA transformation techniques have greatly enhanced the power of classical yeast genetics. It is now possible to isolate any classically defined gene, to alter the yeast genome at will by replacing normal chromosomal sequences with mutated derivatives produced in vitro, and to create DNA molecules that behave as autonomous replicons or minichromosomes. These unique features of the new yeast genetics have been used to study many problems in eukaryotic molecular biology.
189 citations