scispace - formally typeset
Search or ask a question
Topic

Yeast

About: Yeast is a research topic. Over the lifetime, 31777 publications have been published within this topic receiving 868967 citations. The topic is also known as: yeasts.


Papers
More filters
Journal ArticleDOI
TL;DR: Bioinformatics and yeast two-hybrid screening methods were used to identify four novel human proteins (VPS37A–D) that share weak but significant sequence similarity with yeast Vps37p and to demonstrate that V PS37A and VPS37B bind TSG101.

167 citations

Journal ArticleDOI
TL;DR: The results suggest that CDC14 and YNL128W/PTEN/MMAC1 represent two related, but distinct, families of human and yeast phosphatases.

167 citations

Journal ArticleDOI
TL;DR: To improve the expression of the XR gene in yeast (Saccharomyces cerevisiae), its 5′-noncoding sequence containing the genetic elements for transcription and translation was systematically replaced by that from the yeast genes.
Abstract: The intactPichia stipitis xylose reductase gene (XR) has been cloned and expressed inSaccharomyces cerevisiae. The possible further improvement of the expression of thePichia gene in the new host was studied. To improve the expression of the XR gene in yeast (Saccharomyces cerevisiae), its 5′-noncoding sequence containing the genetic elements for transcription and translation was systematically replaced by that from the yeast genes. It was found that thePichia genetic signal for transcription of XR is more effective than the yeast TRP5 promoter, but is about half as effective as the yeast strong promoter of the alcohol dehydrogenase gene (ADC1). However, the nucleotide sequence immediately adjacent to the initiation codon of XR, which controls the translation of the gene product, seemed to be five times less effective than the corresponding sequence of the ADC1 gene. By totally replacing its 5′-noncoding sequence with that of the yeast ADC1 gene, the expression of XR in yeast was found to be nearly ten times higher. Furthermore, the clonedPichia XR described in this article contains very little of its 3′-noncoding sequence. In order to study whether the 3′-noncoding sequence is important to its expression inS. cerevisiae, the intact 3′-noncoding sequences of the yeast xylulokinase gene was spliced to the 3′ end of theP ADC1-XR structural gene. This latter modification has resulted in a twofold further increase in the expression of thePichia XR in yeast.

167 citations

Journal ArticleDOI
TL;DR: The results showed that both yeast culture products increased dry matter digestion, propionic acid production, and protein digestion compared with the control, and specific yeast cultures may have different modes of action.

167 citations

Journal ArticleDOI
28 Mar 2013-Cell
TL;DR: In this article, a prion formed by the Mot3 transcription factor, [MOT3 + ], governs the acquisition of facultative multicellularity in the budding yeast Saccharomyces cerevisiae.

167 citations


Network Information
Related Topics (5)
Saccharomyces cerevisiae
32.1K papers, 1.6M citations
95% related
Escherichia coli
59K papers, 2M citations
90% related
Fermentation
68.8K papers, 1.2M citations
89% related
Amino acid
124.9K papers, 4M citations
87% related
Mutant
74.5K papers, 3.4M citations
86% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20231,445
20223,214
2021816
2020870
2019977
2018968