Topic
Yeast
About: Yeast is a research topic. Over the lifetime, 31777 publications have been published within this topic receiving 868967 citations. The topic is also known as: yeasts.
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TL;DR: Strong evidence is provided for a Far East Asian origin hypothesis of lager yeast, which apparently corresponds better with geography and world trade history than the Patagonian origin hypothesis, as inferred from population genetics and genome sequence analyses.
164 citations
TL;DR: Labeling kinetics of RNA and measurements of the polyadenylated fractions showed that RNA hybridizing to the pBR322 plasmid was polyadenyated to the same extent as RNA hybridization to the ura3+ gene.
164 citations
TL;DR: With the aim of obtaining efficient production and high productivity, the bovinel-lactate dehydrogenase gene (LDH) was introduced into a wild-type Kluyveromyces lactis yeast strain, in which lactic fermentation substituted completely for the pathway leading to the production of ethanol.
Abstract: Interest in the production of L-(+)-lactic acid is presently growing in relation to its applications in the synthesis of biodegradable polymer materials. With the aim of obtaining efficient production and high productivity, we introduced the bovine L-lactate dehydrogenase gene (LDH) into a wild-type Kluyveromyces lactis yeast strain. The observed lactic acid production was not satisfactory due to the continued coproduction of ethanol. A further restructuring of the cellular metabolism was obtained by introducing the LDH gene into a K. lactis strain in which the unique pyruvate decarboxylase gene had been deleted. With this modified strain, in which lactic fermentation substituted completely for the pathway leading to the production of ethanol, we obtained concentrations, productivities, and yields of lactic acid as high as 109 g liter(-1), 0.91 g liter(-1) h(-1), and 1.19 mol per mole of glucose consumed, respectively. The organic acid was also produced at pH levels lower than those usual for bacterial processes.
164 citations
TL;DR: This work has identified TRAP230/ARC240 and TRAP240/ARC250 as the human homologs of Srb8 and Srb9, showing the entire Srb 8/9/10/11 complex is conserved from yeast to humans.
164 citations
TL;DR: Genes encoding proteins involved in vacuolar function, the cell integrity pathway, mitochondrial function, subunits of the co‐chaperone complex GimC and components of the SAGA transcription factor complex were found to be important for the growth of wild‐type Saccharomyces yeast in the presence of ethanol.
Abstract: The Saccharomyces cerevisiae deletion collection was screened for impaired growth on glucose-based complex medium containing 6% ethanol. Forty-six mutants were found. Genes encoding proteins involved in vacuolar function, the cell integrity pathway, mitochondrial function, subunits of the co-chaperone complex GimC and components of the SAGA transcription factor complex were in this way found to be important for the growth of wild-type Saccharomyces yeast in the presence of ethanol. Several mutants were also sensitive to Calcofluor white (14 mutants), sorbic acid (9), increased temperature (5) and NaCl (3). The transcription factors Msn2p and Ars1p, tagged with green fluorescent protein, were translocated to the nucleus upon ethanol stress. Only one of the genes that contain STRE elements in the promoter was important under ethanol stress; this was TPS1, encoding trehalose 6-phosphate synthase. The map kinase of the cell integrity pathway, Slt2p, was phosphorylated when cells were treated with 6% ethanol. Two out of three mutants tested fermented 20% glucose more slowly than the wild-type.
164 citations