Topic
Yeast
About: Yeast is a research topic. Over the lifetime, 31777 publications have been published within this topic receiving 868967 citations. The topic is also known as: yeasts.
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TL;DR: Six lactating Holstein cows fitted with rumen and T-type duodenal cannulas were used in a crossover design to examine effects of yeast culture supplement on production parameters, rumen fermentation, and flow of N to the duodenum, suggesting that yeast culture may alter the AA profile of bacterial protein.
342 citations
TL;DR: Use of the Von Heijne algorithm allowed the identification of 686 open reading frames (ORFs) in the genome of Saccharomyces cerevisiae that encode proteins with a potential N‐terminal signal sequence for entering the secretory pathway.
Abstract: Use of the Von Heijne algorithm allowed the identification of 686 open reading frames (ORFs) in the genome of Saccharomyces cerevisiae that encode proteins with a potential N-terminal signal sequence for entering the secretory pathway. On further analysis, 51 of these proteins contain a potential glycosyl-phosphatidylinositol (GPI)-attachment signal. Seven additional ORFs were found to belong to this group. Upon examination of the possible GPI-attachment sites, it was found that in yeast the most probable amino acids for GPI-attachment are asparagine and glycine. In yeast, GPI-proteins are found at the cell surface, either attached to the plasma-membrane or as an intrinsic part of the cell wall. It was noted that plasma-membrane GPI-proteins possess a dibasic residue motif just before their predicted GPI-attachment site. Based on this, and on homologies between proteins, families of plasma-membrane and cell wall proteins were assigned, revealing 20 potential plasma-membrane and 38 potential cell wall proteins. For members of three plasma-membrane protein families, a function has been described. On the other hand, most of the cell wall proteins seem to be structural components of the wall: responsive to different growth conditions. The GPI-attachment site of yeast slightly differs from mammalian cells. This might be of use in the development of anti-fungal drugs. (C) 1997 John Wiley & Sons, Ltd.
341 citations
TL;DR: Xylitol accumulation in yeasts is sensitive to environmental conditions such as nutrition, temperature, pH, inoculum, substrate and aeration, with the last two being critical for yeast growth and fermentation.
341 citations
TL;DR: The antagonistic action of the CKI gene product on SEC14p function revealed a previously unsuspected influence of biosynthetic activities of theCDP-choline pathway for PC biosynthesis on yeast Golgi function and indicated thatSEC14p controls the phospholipid content of yeast Gol Gi membranes in vivo.
339 citations
TL;DR: Indirect immunofluorescence and subcellular fractionation studies with yeast cells containing the alpha 2-LacZ hybrid proteins indicate that thealpha 2 segment can direct localization of beta-galactosidase to the nucleus, and indicates that theAlpha 2 protein, argued on genetic grounds to be a negative regulator, acts in the yeast nucleus.
337 citations