Topic
Zeatin
About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.
Papers published on a yearly basis
Papers
More filters
TL;DR: Kinetin inhibited appressorium formation apart from the host on nitrocellulose membranes, suggesting that the inhibitory effects of kinetin on fungus development were direct rather than through the host.
30 citations
TL;DR: The efficient regeneration protocol reported here provides an important method of micropropagation of this plant and may be used for a large-scale production of cardenolides and genetic transformation of this valuable medicinal plant for its further improvement.
30 citations
TL;DR: The results are consistent with a model in which Striga germination and embryo growth are limited by low capacity of the seeds to oxidize ACC, and the cytokinins promote ACC conversion into ethylene and consequent Striga Germination by enhancing ACC oxidase activity and/or synthesis.
Abstract: Germination of witchweed (Striga asiatica [L] Kuntze), an important parasitic weed on several poaceous crops, is stimulated by several synthetic and natural compounds We investigated the role of ethylene biosynthesis and action in cytokinin-induced germination Conditioned Striga seeds treated with distilled water, 1-aminocyclopro-pane-1-carboxylic acid (ACC) or the cytokinins thidiazuron (TDZ), trans zeatin (TZ), benzyladenine (BA) and kinetin (KIN) produced little ethylene Treatments with cytokinin-ACC combinations enhanced ethylene production The relative order of activity of the cytokinins in elicitation of the phytohormone was TDZ > TZ > BA > KIN Germination in response to distilled water and ACC treatments was negligible Induction of germination by cytokinins varied from low (0%) to moderate (52%) Seeds treated with cytokinin-ACC combinations displayed high rates of germination The observed germination was positively correlated (γ= 0 8 and 0 9) with ethylene production Germination was reduced by silver thiosulphate (STS) and CoCl2, inhibitors of ethylene action and ACC oxidase, respectively Aminoethoxyvi-nylglycine (AVG), an ACC-synthase inhibitor, reduced TDZ-induced Striga germination However, the inhibitory effect of AVG was overcome by addition of ACC The results are consistent with a model in which Striga germination and embryo growth are limited by low capacity of the seeds to oxidize ACC The cytokinins promote ACC conversion into ethylene and consequent Striga germination by enhancing ACC oxidase activity and/or synthesis
30 citations
TL;DR: The random amplified polymorphic DNA analysis of in vitro and in vivo plants showed four clusters of similarity and the largest number of regenerated plants was obtained from the embryos cultivated on media enriched with meta‐topolin and benzyladenine.
Abstract: Cytokinins are growth regulators that stimulate cell division and control morphogenesis in plants, however their role in regulating secondary metabolism is not well studied. The influence of various cytokinins (benzyladenine, zeatin, kinetin, meta-topolin, thidiazuron) and culture systems (solid and temporary immersion RITA (R) system) on the quality Leucojum aestivum plant regenerated from somatic embryos was investigated. The largest number of regenerated plants (181.6 and 168.8) was obtained from the embryos cultivated on media enriched with meta-topolin and benzyladenine. Thidiazuron and meta-topolin led to the highest number of normally developed plants (94.8 and 90.6). The random amplified polymorphic DNA analysis of in vitro and in vivo plants showed four clusters of similarity. The highest biomass (growth index: 2.49) was obtained with the temporary immersion RITA (R) system. Alkaloid extracts were analyzed by LC-MS, leading to the quantification of galanthamine and lycorine both in plant materials and in liquid media. The highest contents of galanthamine (0.05% dry weight) were observed in plants cultivated in the presence of thidiazuron in bioreactor system. Galanthamine was accumulated (highest content 0.05% dry weight) in plants cultivated in the presence of thidiazuron in bioreactor system whereas lycorine was synthetized mainly in plants cultivated on solid media.
30 citations
TL;DR: Rhododendron simsii ‘Hellmut Vogel’ was regenerated using different types of explants, auxins and cytokinins and the duration of the callus induction phase was negatively correlated with the regeneration capacity.
Abstract: Rhododendron simsii ‘Hellmut Vogel’ was regenerated using different types of explants, auxins and cytokinins. After a callus induction phase, with 2,4-dichlorophenoxyacetic acid or α-naphthaleneacetic acid, adventitious shoot regeneration was obtained on a medium supplemented with thidiazuron or zeatin. With thidiazuron shoots were small and a subsequent elongation step was required before rooting. An elongation step was not required when zeatin was used. The duration of the callus induction phase was negatively correlated with the regeneration capacity.
30 citations