Zeatin

About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.

Growth optimization and organogenesis of Gerbera jamesonii Bolus ex. Hook f. in vitro.

Abstract: Regeneration potentials in Gerbera jamesonii Bolus ex Hook f from tissues culture system was studied using leaf, petiole and root explants In vitro regeneration, callus induction and root formation were optimized by manipulation of growth regulators during organogenesis Various kinds of plant growth regulators such as 6-Benzylaminopurine (BAP), alpha-Naphthalene acetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2,4-D), Indole-3-acetic acid (IAA), Indole-3-Butyric acid (IBA), N6-[2-Isopentenyl]adenine (2iP), Kinetin and Zeatin were used to initiate cultures These plant growth regulators were added to Murashige and Skoog medium in different combinations and concentrations Adventitious shoots were obtained from petiole explants cultured on Murashige and Skoog (MS) medium supplemented with 20 mg L(-1) BAP and 05 mg L(-1) NAA Effectiveness of shoot regeneration medium, type of growth regulator used and duration of induction period were investigated Leaf explants cultured on MS medium supplemented with 10 mg L(-1) BAP and 20 mg L(-1) 2, 4-D showed the best results for callus induction Root explants were found to be non-regenerative in all experiments conducted Petiole segment was identified as the best explant for regeneration of this species Regenerated plants were rooted on Murashige and Skoog basal medium Plantlets were then transferred to field with 75% survival rate

Micropropagation of Carlina acaulis L.

Abstract: An efficient shoot propagation system for Carlina acaulis was developed in this study. The experimental material consisted of shoot tips and fragments of hypocotyls excised from 10-day-old seedlings. The explants were transferred to proliferation medium supplemented with different types of cytokinins: 6-benzylaminopurine (BA, 4.4 or 13.3 μM), kinetin (Kn, 4.7 or 13.9 μM) and zeatin (Zea, 4.6 or 13.7 μM) in combination with naphthaleneacetic acid (0.54 μM NAA). The morphogenetic response was best in culture on medium supplemented with 13.3 μM BA, and shoot organogenesis frequency was highest for shoot tips (100%). On average, 7.5 shoots were induced per explant of the initial material, and the multiplication rate in five subsequent subcultures was 6.1. Shoot length was lower in culture with BA in the medium than with Kn or Zea. Plantlets rooted with 60% frequency in vitro on full-strength MS medium and with 55.3% frequency ex vitro. Reduction of the mineral salt concentration (1/2MS) stimulated rhizogenesis. Addition of auxins stimulated both the frequency and number of roots per shoot, but only in combination with full-strength MS medium. Regenerated plants were able to flower and gave viable seeds.

Identification of Cell Division Inducing Compounds from Coconut Milk

Abstract: The use of chromatographic techniques coupled with mass spectrometry, infra-red analysis and nuclear magnetic resonance revealed that the amino acid phenylalanine might be responsible for a large proportion of the cell division activity of coconut milk as determined by the soybean bioassay. The observation that zeatin can be removed very readily by partitioning against ethyl acetate at alkaline pH values, cautions against inclusion of this method as a purification step for extracting cytokinins from plant extracts. Mass spectrometry revealed the presence of an unidentified cell division substance with a molecular ion of 279 and which co-chromatographed with zeatinriboside on Sephadex LH-20.

Shoot Induction from Axillary Shoot Tip Explants of Fig (Ficus carica) cv. Japanese BTM 6.

Abstract: Fig, or Ficus carica, is a fruit tree from the Moraceae family and is widely grown in tropical and subtropical regions of the world. Fig plants are mainly propagated through grafting, air layering, and hardwood cutting whereby these methods were found to be less efficient. Plant tissue culture is efficient method to propagate plants, particularly to produce true-to-type platelets for mass multiplication. The aim of this study is to induce multiple shoot formation on Ficus carica cv. Japanese BTM 6 through identifying and optimising the concentrations of 6-Benzylaminopurine (BAP) and Zeatin suited for shoot formation. The axillary shoot tip explants were cultured in MS media supplemented with different concentrations of BAP and Zeatin (0, 0.5, 1.0, 1.5 and 2.0 mg/L) to determine the optimal concentration for the formation of multiple shoots. Number of shoots per explants and the differences in shoot height of explants were calculated after 8 and 12 weeks of culture respectively. Of all the treatments of BAP, MS media containing with 2 mg/L BAP marked the highest number of shoots per explant with the average value of 1.67 ± 0.33 while 1.5 and 2 mg/L of BAP produced the highest differences in shoot height with 0.51 ± 0.08 cm and 0.51 ± 0.07 cm after 12 weeks respectively. Murashige and Skoog (MS) media supplemented with 2 mg/L Zeatin showed the highest production of multiple shoots and differences in shoot height with the average of 0.83 ± 0.219 and 0.32 ± 0.04 cm respectively among all the different treatments of Zeatin. In this study, BAP performed better in shoot induction and elongation as compared to Zeatin for the cultivar Japanese BTM 6.