Zeatin

About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.

Direct in vitro regeneration of lentil (Lens culinaris Medik.).

Abstract: Establishment of an efficient and reproducible regeneration protocol is one of the basic prerequisites for genetic transformation of any crop plant. In vitro culture of lentil has proven to be difficult. In spite of a number of reports on the regeneration of this plant, very few satisfying and reproducible protocol has yet been reported. This study carried out for investigation of different hormone treatments and explants in order to establish a reproducible protocol for indirect in vitro regeneration of the cultivar Gachsaran (commonly grown in Iran). For this purpose, the effects of 13 different hormone treatments and 4 explants on callus induction and regeneration were studied. Callus with the highest fresh and dry weight was produced on modified Murashige and Skoog (MS) medium containing 1 mg/L α-naphthaleneacetic acid (NAA)and 1 mg/L Zeatin (medium E). Among the explants, decapitated embryos attached to 1/4 of the cotyledon (DEAC) produced callus with the highest fresh and dry weights. In the regeneration stage, calli induced on media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) alone or in combination with other hormones did not result in shooting or rooting responses. The highest shooting and rooting responses (75%) were observed for callus induction medium E, using decapitated embryos with a quarter of the cotyledon as the explant.

Micropropagation of globe artichoke ( Cynara scolymus L.) from underground dormant buds (“ovoli”)

Abstract: Side shoots excised from underground dormant buds ofCynara scolymus L. were used as primary explants to establishin vitro cultures. A 3×3 factorial experiment with all possible combinations of three concentrations (0.5, 1.0, 2.0 mg/liter or 2.22, 4.44, 8.88 μM) ofN 6-benzyladenine (BA) and three concentrations (0, 0.1, 0.2 mg/liter or 0, 0.54, 1.07 μM) of 1-naphthaleneacetic acid (NAA) was used to determine the optimum growth regulator combination for shoot multiplication. The highest rate of axillary shoots was induced on Murashige and Skoog agar medium supplemented with 0 mg NAA/liter and 1.0 mg BA/liter (4.44 μM). Other cytokinins tested (kinetin, zeatin, and 2-isopentenyl-adenine were less effective than BA in inducing axillary shoot growth. Up to 60% of elongated microshoots rooted after 5 weeks on 1/2 MS agar medium supplemented with 2 mg/liter (11.42 μM) indole-3-acetic acid (IAA). Seventy percent of rooted plantlets were transferred successfully into soil. Plants are under evaluation for their genetic uniformity and clonal fidelity.

Electrochemical aptasensors for zeatin detection based on MoS2 nanosheets and enzymatic signal amplification.

Abstract: A simple and sensitive electrochemical aptasensor was constructed for zeatin detection, where MoS2 nanosheets were used as the immobilization matrix for gold nanoparticles (AuNPs), and AuNPs were employed as the immobilization matrix to probe DNA. After the aptamer DNA and assist DNA hybridized with probe DNA, Y-type DNA can be formed with two biotins at the terminals of aptamer DNA. Then, avidin modified alkaline phosphatase (Avidin-ALP) can be further modified on the electrode surface through the biotin and avidin interaction. Under the catalytic effect of ALP, p-nitrophenylphosphate disodium (PNPP) can be hydrolyzed to produce p-nitrophenol (PNP). However, in the presence of zeatin, the formed Y-type DNA can be destroyed due to the formation of the zeatin–aptamer conjugate, which further reduces the amount of PNP and leads to the decrease of the oxidation signal of PNP. Under the optimum conditions, the change of the oxidation peak current of PNP was inversely proportional to the logarithm value of zeatin concentration in the range of 50 pM–50 nM. The detection limit was calculated to be 16.6 pM. This electrochemical method also showed good detection selectivity and stability. The potential applicability of this method was proved by detecting zeatin in real samples.

Modification of cytokinin levels in potato via expression of the Petunia hybrida Sho gene.

Abstract: The Sho gene from Petunia hybrida encodes an enzyme for cytokinin synthesis. Here we report on the effects of Shogene expression on potato development. In contrast to transgenic potato expressing the Agrobacterium ipt gene, moderate Sho expression resulted in sufficient root development that allowed the cultivation of the Sho transformants in soil. The most pronounced effects detectable in these lines were an enhanced shoot production, delayed tuber formation, significant reduction in tuber size, and inhibition of tuber dormancy. Sho expression predominantly associated with a strong increase in 2iP glucosides, accompanied by an increase in zeatin glucosides in lines with very high Sho expression levels. The data demonstrate that it is possible to produce viable plants with enhanced cytokinin levels via constitutive Sho expression, which allows an assessment of cytokinin effects in all organs.