Topic
Zeatin
About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.
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TL;DR: The results suggest that roots regulate meristematic functioning through cytokinins, which exert the same influence as roots on cotyledonary buds cultured in vitro.
Abstract: Influence of roots on the vegetative or floral development of cotyledonary buds of Scrofularia arguta Sol.: A possible cytokinin role. This study shows that the presence of “nonabsorbing roots” insures a vegetative development of cotyledonary buds cultured in vitro whereas buds growing without roots produce flowers early. In the same way, roots suppress floral expression of axillary meristems of the same cotyledonary buds and induce these buds to vegetative functioning. Various trophic modifications in the culture medium are ineffective on non-rooted buds as also are gibberellin As and adenine. On the contrary, several cytokinins (kinetin, benzyladenine and zeatin) exert the same influence as roots. These results suggest that roots regulate meristematic functioning through cytokinins.
12 citations
TL;DR: A method for micropropagation of a selected new cultivar Xiang Lin G1 through shoot culture is established, based on existing meristems, which could lead to rapid propagation of the high yield cultivars on a year-round basis and quickly increase their production hectares.
12 citations
TL;DR: Effect of plant growth regulators on morphogenesis was studied using callus derived from inflorescence segments of sugarcane cv.
Abstract: Effect of plant growth regulators on morphogenesis was studied using callus derived from inflorescence segments of sugarcane cv. Co-91010 and CoC-671 cultured on MS medium supplemented with 2,4-D, malt extract, L-glutamine, casein hydroly sate and coconut water (CW). Comparison was made of different media combinations of CW (5,10%), kinetin (lmg/l), zeatin (0.5mg/l) andTDZ (0.5mg/l) to study callus growth and regeneration. Results showed that CW (5%) + zeatin (0.5mg/l) was more effective over other growth regulators for callus induction while CW 5% was found effective for the regeneration of healthy plants in cv. Co-91010. Different levels of proline and thioproline were tested on the induction of embryogenie callus and subsequent plant regeneration in var. CoC-671. Proline at 2.61M showed vigorous growth of embryogenie callus compared to thioproline. Plants regenerated were hardened and grown in the green house to maturity.
12 citations
TL;DR: Hydrogen cyanamide has been used to break bud and seed dormancy and to improve rooting in several species, responses usually associated with the action of plant hormones, and its action upon ABA, CKs and IAA endogenous levels is discussed.
Abstract: Hydrogen cyanamide (HC) has been used to break bud and seed dormancy and to improve rooting in several species, responses usually associated with the action of plant hormones. However, very few studies have measured endogenous hormones after HC treatment. Therefore, pea (Pisum sativum L.) seedlings with two fully developed internodes above the first leaf were sprayed with 0, 0.1 and 0.3% (v/v) HC. Endogenous concentration of indole-3-acetic acid (IAA), abscisic acid (ABA), zeatin/zeatin riboside and N 6 (∆ 2 -isopentenyl) adenine/ N 6 (∆ 2 -isopentenyl) adenosine were measured by radioimmunoassay 31 and 80 h after HC treatment. A significant increase in ABA and cytokinin (CK) levels was observed 31 h after treating the plants with 0.3% HC. Small necrotic spots were also noticed in this treatment, thus revealing a toxic effect of this treatment. Additionally, at 80 h, a significant increase in IAA was found for both HC concentrations applied. The action of HC upon ABA, CKs and IAA endogenous levels is discussed.
12 citations
TL;DR: BA and TDZ in the forcing solution enhanced subsequentin vitro axillary shoot initiation of nodal explants taken from forced stems by doubling the number of shoots produced per explant, thus expediting the micropropagation of A. Sieboldianus.
Abstract: A. Sieboldianus (5-leaf aralia) is recalcitrant for micropropagation, but has very good landscaping potential. This research was conducted with the following objectives: (1) to study effects of BA, TDZ, CPPU, 2iP, kinetin and zeatin in woody plant medium on the performance of softwood shoot nodal explants produced by field grown 5-leaf aralia plants; (2) to investigate influences of BA or TDZ in the forcing solution on subsequentin vitro shoot initiation of nodal explants taken from forced softwood growth. Shoot initiation of softwood nodal explants from field-grown plants was promoted by adding BA, TDZ or CPPU to the culture medium. Kinetin, zeatin and 2iP were ineffective for micropropagation ofA. Sieboldianus. The forced softwood growth for use as explants was “primed” by forcing dormant stems in solution containing 200 mg 8-HQC per liter plus 2% sucrose, 44.4, 222, or 444 µM BA, or 45.4, 227, or 454 µM TDZ. BA and TDZ in the forcing solution enhanced subsequentin vitro axillary shoot initiation of nodal explants taken from forced stems by doubling the number of shoots produced per explant to 3.3 from 1.65 shoots per explant taken from field grown plants. This forcing solution technique also reduced the time needed from culture initiation to potted plants to half of the time needed for the conventional micropropagation method (12 to 14 vs. 25 to 27 weeks), thus expediting the micropropagation ofA. Sieboldianus.
12 citations