Topic
Zeatin
About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.
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TL;DR: An effective protocol for Agrobacterium-mediated genetic transformation of cucumber was optimised and it was observed that transgenic plants had generally sterile flowers.
Abstract: Cucumber plant (Cucumis sativus L.) was transformed by EHA101 strain of A. tumefaciens harboring the binary vector pGA482GG plasmid carrying the marker genes the neomycin phosphotransferase II (npt II) determining resistance to kanamycin and p-ghicuronidase (GUS). Cotyledon and hipocotyl segments of in vitro grown seedlings were used as explants. These tissue segments were cultured on MS medium containing different hormone combinations and concentrations to identify optimum shoot regeneration condition. The highest number of shoot regeneration was observed on MS medium containing 2 mg/1 zeatin. In the tested explant tissues, the highest shoot formation was obtained from the proxiroal ends of cotyledon leaves which were cut transversely into two halves. Plant tissue segments were co-cultivated with A. tumefaciens for 2 days. Tissues, selected on the MS medium containing kanamycin (50 mg/1) were tested by histochemical GUS assay. Shoots regenerated from the transgenic tissue were cultured on the bas...
11 citations
TL;DR: The results suggest that cytokinins play a significant role in KClO3 induced “off season” floral induction in longan, and this role may not be restricted to the biosynthesis of these hormones but also to their mobilization and accumulation in the SAB of newly flushing shoots.
11 citations
TL;DR: The results indicate the feasibility of clonal propagation of meadowfoam and suggest that differentiated secondary embryos from immature seeds can be passed on to differentiated embryos from mature seeds.
Abstract: Developing embryos from immature seeds were excised and cultured. Optimal proliferation of differentiated secondary embryos occurred on Murashige-Skoog media containing 7% sucrose, 0.1 μM 2,4-D, and 0.1–1.0 μM zeatin. Higher levels of auxins inhibited embryo proliferation. Secondary embryos were subcultured to produce more embryos. The results indicate the feasibility of clonal propagation of meadowfoam.
11 citations
TL;DR: This is the first report of successful synthetic seed formation and germination of S. vomeracea in in vitro micropropagation, synthetic seeds formation, germination in soil, and acclimatization to ambient conditions.
Abstract: Orchids are considered recalcitrant plants in in vitro propagation. Due to the lack of appropriate micropropagation techniques for mass production and damage to their ecological distribution posed by local gatherers, these species are threatened with extinction, including Serapias vomeracea (Burm.f.) Briq. In this research, we put forward a complete micropropagation method covering in vitro micropropagation, synthetic seed formation, germination in soil, and acclimatization to ambient conditions. To the best of our knowledge this is the first report of successful synthetic seed formation and germination of S. vomeracea. Initially, seeds were germinated in different culture media and also media supplemented with different concentrations of plant growth regulators. Effects of plant growth regulators on tuber formation, glucomannan contents, and different growth parameters were evaluated throughout the study. The best germination rate (84.03%) was achieved on Orchimax including activated charcoal medium and supplemented with 2.0 mg/L 6-benzyladenine. The longest shoot elongation amongst plantlets was observed on the same medium supplemented with 0.25 mg/L thidiazuron, whereas 2.0 mg/L indole-3-butyric acid favored leaf formation. Higher indole-3-butyric acid concentrations were found to be more effective in the formation and elongation of roots. Orchimax medium supplemented with zeatin (2.0 mg/L) was superior to the others in terms of tuber formation and glucomannan content therein. Adaptation of seedlings to soil conditions and germination abilities of synthetic seeds were also studied and seedlings were successfully acclimatized and adapted to soil conditions.
11 citations
TL;DR: Exogenous cytokinins were extracted and their amounts varied in 2 groups of fungi and it is presumed that they may have a bearing on the onset of senescence in mushrooms.
11 citations