Topic
Zeatin
About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.
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TL;DR: Caution should be exercised when expansion of radish cotyledons is employed as a cytokinin assay if NaCl or KCl are present in varying quantities; the interaction between cytokinins and NaCl appeared to be additive.
11 citations
TL;DR: Less efficient in vitro regeneration was observed when hypocotyl and shoot sections, and shoot apices were induced to form callus and plants by organogenesis, and these plants could not be established in a potting mix.
Abstract: A comparison of methods for efficient in vitro regeneration of Australian-grown chickpea (Cicer arietinum L.) cultivars was undertaken. The most efficient regeneration system was one where immature cotyledon and embryonic axis explants, 14-21 days post-pollination, were cultivated on Murashige and Skoog's salts with Gamborg's vitamins, 1.0, 3.0 or 5.2 mg L-1 zeatin, 0 or 35 μg L-1 indole-3-acetic acid, 30 g L-1 sucrose and 8 g L-1 Phytagar. The first embryoid structures appeared after 2 weeks of culture at 25 ± 1°C in dim light (150 μmol m-2 s-1) and formed directly on the edges of the immature cotyledons or petiole stumps. Between 10 and 20 structures were produced on each cotyledon explant in two cultivars, however, the embryogenic structures which developed on cv. Narayen were more efficiently transformed into shoots than far cv. Amethyst. An efficient regeneration medium (2 mg L-1 naphthaleneacetic acid, 1/2 Murashige and Skoog's salts with Gamborg's vitamins, and 0.5 g L-1 activated charcoal) was used to develop a portion of the shoots into morphologically normal plants growing in a vermiculite and soil potting mix in a growth room. Less efficient in vitro regeneration was observed when hypocotyl and shoot sections, and shoot apices were induced to form callus and plants by organogenesis. These plants could not be established in a potting mix. The amount and type of callus produced varied between explant type and cultivar.
11 citations
01 Feb 2002
TL;DR: In this paper, a protocol for efficient plant regeneration of Iris pumila L. was developed via somatic embryogenesis and/or organogenesis from suspension cultures, which was achieved by leaf-base culture of in vitro grown plants on solid Murashige and Skoog (MS) medium supplemented with 3 % sucrose and (in mgL): inositol 100, pantothenic acid 10, nicotinic acid 5, vitamin B1 2, vitamin b6 1, casein hydrolysate 250, proline and kinetin (1.0 mg
Abstract: A protocol for efficient plant regeneration of Iris pumila L. was developed via somatic embryogenesis and/or organogenesis from suspension cultures. Induction of embryogenic calli was achieved by leaf-base culture of in vitro grown plants on solid Murashige and Skoog (MS) medium supplemented with 3 % sucrose and (in mgL): inositol 100, pantothenic acid 10, nicotinic acid 5, vitamin B1 2, vitamin B6 1, casein hydrolysate 250, proline 250, 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (1.0 mgL, each). Cell suspensions were established and maintained in MS liquid medium with the same content of 2,4-D and kinetin as used for induction and proliferation of embryogenic calli. After three subcultures stable suspension cultures were successfully established and maintained by subculturing every 3 weeks. The suspension cultures were initially composed of single cells, bi-, three and multicellular proembryos and cell aggregates. In prolonged suspension cultures (6-8 weeks) three types of embryogenic calli were observed: yellow, compact (Type I); yellow-green, friable (Type II) and white, friable (Type III). The effect of cytokinins (zeatin 0.05; 0.1; 0.2 and / or 6-benzylaminopurine-BAP, 0.1 and 1.0 mgL, respectively) on plant regeneration of these three types of calli were investigated. Friable (Type II and III) suspension derived calli have the highest morfogenic potential. During the regeneration process, two different regeneration pathways were observed: somatic embryogenesis and/or organogenesis dependent on used cytokinin. Germination of normally developed somatic embryos was achieved on MS solid medium without hormones. Potted plants of I. pumila grew normally and flowered.
11 citations
TL;DR: The ribose moiety and the saturated side chain apparently influence cytokinin transport and physiological activity in Citrus seedlings.
Abstract: The influence of vesicular-arbuscular mycorrhizal symbiosis on cytokinin activity in Citrus jambhiri Lush, seedlings was investigated. C. jambhiri inoculated with cultures of Glomus caledonium (Nicol. and Gerd.), G. epigaeum (Dan. and Trappe), G. etunicatum (Becker and Gerd.), G. fasciculatum Thaxt. (Gerd, and Trappe) or G. mosseae (Nicol and Gerd.) was grown from seed for 105 days in a glasshouse. Cytokinin activity in roots and leaves of seedlings was analyzed using high-performance liquid chromatography, mass spectrometry and a bioassay. Seedling leaf tissue had greater cytokinin activity than root tissue. Zeatin, zeatin riboside, and their dihydro- and glucoside derivatives were isolated from leaves of 105-day-old seedlings inoculated with G. fasciculatum and G. mosseae. Cytokinin activity in roots and leaves was associated with differences in seedling total dry weight and vesicular-arbuscular mycorrhizal colonization. The ribose moiety and the saturated side chain apparently influence cytokinin transport and physiological activity in Citrus seedlings.
11 citations
TL;DR: Both cotyledons and the root tip apparently control the initiation and emergence of the lateral roots, and it appears as if cytokinins of root tip origin inhibit lateral root formation in the 10–15 mm apical section of the primary root.
11 citations