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Zeatin

About: Zeatin is a research topic. Over the lifetime, 2467 publications have been published within this topic receiving 64092 citations. The topic is also known as: Zeatin & (E/Z)-zeatin.


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Journal Article
TL;DR: In this paper, the adventitious buds were induced successfully on MS medium + 6-BA (N 6 -benzyladenine) 4.0 mg l - 1 + KT(kinetin) 2.
Abstract: Explants of bitter melon can produce three types of callus: green, yellow green and fragile yellow callus. During the period of inducing callus, the explants are not sensitive to the kinds and proportions of phytohormone.The rate of callus inductivity is about 90.0% . However, in the process of callus differentiating adventitious bud, the kind, proportion and quantity of phytohormone and the type of callus made different result. The adventitious buds were induced successfully on MS medium + 6-BA (N 6 -benzyladenine) 4.0 mg l - 1 + KT(kinetin) 2.0 mg l - 1 ) by yellow green callus. The frequency was about 66.7 %. Yellow callus did not differentiated adventitious bud. The frequency of green callus differentiation was very low ( < 15.0 % ) even under the most suitable conditions. The MS + ZT (zeatin) 5.0 mgl - 1 + KT 0. 5 mgl - 1 medium was suitable for the proliferation of bud and three weeks later, the coefficient of proliferation was about 5-6. The ½ MS + ZT 0.02 mgl - 1 or ½ MS media were suitable for in vitro rooting of shoot, the shoot on them could produce 6 - 7 new roots in three weeks. After plantation test, the survival rate of tube plantlets was about 70% and their characteristics were the same as those from seed by field test.

8 citations

01 Jul 2012
TL;DR: In vitro propagation of Boucerosia diffusa Wight (Apocynaceae), an endemic medicinal plant of Western Ghats, was carried out and the developed micropropagation protocol would help ex situ conservation of this endemic species.
Abstract: In vitro propagation of Boucerosia diffusa Wight (Apocynaceae), an endemic medicinal plant of Western Ghats, (Tamil Nadu, India) was carried out. Nodal explants were cultured on MS medium supplemented with cytokinins [benzyl aminopurine (BAP), kinetin (Kn), thidiazuron (TDZ) & zeatin] either individually or in combinations. Shoot buds proliferated on MS medium+BAP (8.87 μM) and repeated subculture on MS+BAP (8.87 μM)+TDZ (2.27 μM) promoted the shoot multiplication. The regenerated shoots were then rooted on MS+NAA (2.69 μM). The micropropagated planlets were hardened with the survival success rate of 56%. The developed micropropagation protocol would help ex situ conservation of this endemic species.

8 citations

Journal ArticleDOI
TL;DR: Zeatin-induced division and differentiation was accompanied by up to 12-fold increases in the amount of radioactivity in poly Saccharide of isolated membrane fractions, and the pattern of distribution of label between the sugars of this polysaccharide was qualitatively different in the presence of zeatin.
Abstract: Changes have been induced in the polysaccharides laid down in the cell walls of lettuce pith by administration of the hormones auxin and zeatin. This polysaccharide derives from the membrane systems of the cells and radioactive precursor has been used to follow the accompanying changes which occurred in the polysaccharide contained within isolated Golgi and endoplasmic reticulum fractions. Zeatin-induced division and differentiation was accompanied by up to 12-fold increases in the amount of radioactivity in polysaccharide of isolated membrane fractions, and the pattern of distribution of label between the sugars of this polysaccharide was qualitatively different in the presence of zeatin. The change in this pattern was evident in the Golgi fraction at an earlier stage in the induction of the response than that in the endoplasmic reticulum.

8 citations

01 Jan 2016
TL;DR: The "SRQ-93" genotype showed a significant effect on the embryogenic response, and the formation and development of somatic embryos was achieved, whereas the genotypes "Bertoni" and "Morita II" only yielded embryogenic and non-embryogenic calli, respectively.
Abstract: Stevia rebaudiana (Asteraceae) is a plant of economic importance because of its medicinal properties and the presence of sweetener compounds on its leaves. These compounds can be a substitute for sucrose in a wide variety of products used by persons with diabetes and obesity problems. To standardize an efficient and effective propagation method for the different Stevia genotypes grown in Colombia, this study evaluated the effect of different combinations of the plant growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), 6-(gamma, gamma-dimethylallylamino) purine (2iP) and Zeatin on the induction and development of somatic embryos. Adenine and coconut water were also evaluated as supplements in the basal culture medium Murashige and Skoog Basal Salt Mixture (MS) with glutamine. The combination of 2,4-D (18.09 µM) and 2iP (7.38 µM) produced the highest number of somatic embryos per explant, which had well-defined characteristics. The genotype showed a significant effect on the embryogenic response. In the "SRQ-93" genotype, the formation and development of somatic embryos was achieved, whereas the genotypes "Bertoni" and "Morita II" only yielded embryogenic and non-embryogenic calli, respectively. The conversion to seedlings was achieved on the regeneration medium containing gibberellic acid (GA 3 ) (0.29 µM) and activated charcoal.

8 citations

Journal ArticleDOI
TL;DR: In this article, the authors found that salinity stress significantly increased zeatin and jasmonic acid levels in leaves and roots of Pongamia pinnata, respectively, and showed an effective apoplastic sodium sequestration in the roots.
Abstract: Salinity stress results in significant losses in plant productivity and loss of cultivable lands. Although Pongamia pinnata is reported to be a salt-tolerant semiarid biofuel tree, the adaptive mechanisms to saline environments are elusive. Despite a reduction in carbon exchange rate (CER), the unchanged relative water content provides no visible salinity induced symptoms in leaves of hydroponic cultivated Pongamia seedlings for 8 days. Our Na+ -specific fluorescence results demonstrated that there was an effective apoplastic sodium sequestration in the roots. Salinity stress significantly increased zeatin (~5.5-fold), and jasmonic acid (~3.8-fold) levels in leaves while zeatin (~2.5-fold) content increased in leaves as well as in roots of salt-treated plants. Metabolite analysis suggested that osmolytes such as myo-inositol and mannitol were enhanced by ~12-fold in leaves and roots of salt-treated plants. Additionally, leaves of Pongamia showed a significant enhancement in carbohydrate content, while fatty acids were accumulated in roots under salt stress condition. At the molecular level, salt stress enhanced the expression of genes related to transporters, including the Salt Overly Sensitive 2 gene (SOS2), SOS3, vacuolar-cation/proton exchanger, and vacuolar-proton/ATPase exclusively in leaves, whereas the Sodium Proton Exchanger1 (NHX1), Cation Calcium Exchanger (CCX), and Cyclic Nucleotide Gated Channel 5 (CNGC5) were up-regulated in roots. Antioxidant gene expression analysis clearly demonstrated that peroxidase levels were significantly enhanced by ~10-fold in leaves, while Catalase and Fe-superoxide Dismutase (Fe-SOD) genes were increased in roots under salt stress. The correlation interaction studies between phytohormones and metabolites revealed new insights into the molecular and metabolic adaptations that confer salinity tolerance to Pongamia.

8 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202333
2022103
202135
202034
201932
201848